Initiation and Characterization of Keratinocyte Cultures from Biopsies of Normal Human Conjunctiva

2002 ◽  
Vol 74 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Barbara C. Risse Marsh ◽  
Mina Massaro-Giordano ◽  
Christine M. Marshall ◽  
Robert M. Lavker ◽  
Pamela J. Jensen
Keyword(s):  
Normal Human ◽  
Human Conjunctiva

Characterization of a Spontaneously Immortalized Cell Line (IOBA-NHC) from Normal Human Conjunctiva

2003 ◽  
Vol 44 (10) ◽  
pp. 4263 ◽  
Author(s):  
Yolanda Diebold ◽  
Margarita Calonge ◽  
Amalia Enri´quez de Salamanca ◽  
Sagrario Callejo ◽  
Rosa M. Corrales ◽  
...  
Keyword(s):  
Cell Line ◽  
Immortalized Cell Line ◽  
Normal Human ◽  
Human Conjunctiva ◽  
Immortalized Cell

Purification and characterization of the yeast-expressed erythropoietin mutant Epo (R103A), a specific inhibitor of human primary hematopoietic cell erythropoiesis

Blood ◽  
2002 ◽  
Vol 99 (12) ◽  
pp. 4400-4405 ◽  
Author(s):  
Suzanne Burns ◽  
Murat O. Arcasoy ◽  
Li Li ◽  
Elizabeth Kurian ◽  
Katri Selander ◽  
...  
Keyword(s):  
Animal Models ◽  
Specific Inhibitor ◽  
Competitive Inhibitor ◽  
Erythropoietin Receptor ◽  
Single Amino Acid ◽  
Human Cd34 ◽  
Unit Formation ◽  
Dependent Cell ◽  
Normal Human

A drug that specifically inhibits erythropoiesis would be clinically useful. The erythropoietin (Epo) mutant Epo (R103A) could potentially be used for this purpose. Epo (R103A) has a single amino acid substitution of alanine for arginine at position 103. Because of this mutation, Epo (R103A) is only able to bind to one of the 2 subunits of the erythropoietin receptor (EpoR) homodimer and is thus a competitive inhibitor of Epo activity. To produce large quantities of Epo (R103A) to test in animal models of thalassemia and sickle cell disease, we expressed and purified recombinant Epo (R103A) from the yeast Pichia pastoris. Using this method milligram quantities of highly purified Epo (R103A) are obtained. The yeast-expressed Epo (R103A) is properly processed and glycosylated and specifically inhibits Epo-dependent cell growth and125I-Epo binding. Epo (R103A) does not, however, directly induce apoptosis in 32D cells expressing EpoR. Epo (R103A) inhibits erythropoiesis of human CD34+ hematopoietic cells and completely blocks erythroid burst-forming unit formation in normal human bone marrow colony assays. Yeast-expressed Epo (R103A) is a specific inhibitor of primary erythropoiesis suitable for testing in animal models.

Architectural and Immunohistochemical Characterization of Biliary Ductules in Normal Human Liver

2009 ◽  
Vol 18 (10) ◽  
pp. 1417-1422 ◽  
Author(s):  
Katalin Dezső ◽  
Sándor Paku ◽  
Veronika Papp ◽  
Eszter Turányi ◽  
Peter Nagy
Keyword(s):  
Human Liver ◽  
Normal Human Liver ◽  
Normal Human

scholarly journals Characterization of an activated human ros gene.

1986 ◽  
Vol 6 (9) ◽  
pp. 3109-3116 ◽  
Author(s):  
C Birchmeier ◽  
D Birnbaum ◽  
G Waitches ◽  
O Fasano ◽  
M Wigler
Keyword(s):  
Gene Transfer ◽  
Protein Kinases ◽  
Transmembrane Domain ◽  
Extracellular Domain ◽  
Kinase Domain ◽  
Specific Protein ◽  
Normal Human

A human oncogene, mcf3, previously detected by a combination of DNA-mediated gene transfer and a tumorigenicity assay, derives from a human homology of the avian v-ros oncogene. Both v-ros and mcf3 can encode a protein with homology to tyrosine-specific protein kinases, and both mcf3 and v-ros encode a potential transmembrane domain N terminal to the kinase domain. mcf3 probably arose during gene transfer from a normal human ros gene by the loss of a putative extracellular domain. There do not appear to be any other gross rearrangements in the structure of mcf3.

Characterization of B7H6, an endogenous ligand for the NK cell activating receptor NKp30, reveals the identity of two different soluble isoforms during normal human pregnancy

Immunobiology ◽  
2018 ◽  
Vol 223 (1) ◽  
pp. 57-63 ◽  
Author(s):  
Jorge Gutierrez-Franco ◽  
Rodolfo Hernandez-Gutierrez ◽  
Miriam Ruth Bueno-Topete ◽  
Jesse Haramati ◽  
Rosa Elena Navarro-Hernandez ◽  
...  
Keyword(s):  
Nk Cell ◽  
Endogenous Ligand ◽  
Human Pregnancy ◽  
Normal Human ◽  
Activating Receptor

Characterization of growth and differentiation of normal human submandibular gland epithelial cells in a serum-free medium

1993 ◽  
Vol 54 (2) ◽  
pp. 143-153 ◽  
Author(s):  
Masaya Okura ◽  
Kanemitsu Shirasuna ◽  
Tsutomu Hiranuma ◽  
Hideo Yoshioka ◽  
Hirokazu Nakahara ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Submandibular Gland ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Normal Human

scholarly journals Construction and Characterization of a Pseudomonas aeruginosa Mucoid Exopolysaccharide-Alginate Conjugate Vaccine

2003 ◽  
Vol 71 (7) ◽  
pp. 3875-3884 ◽  
Author(s):  
Christian Theilacker ◽  
Fadie T. Coleman ◽  
Simone Mueschenborn ◽  
Nicolas Llosa ◽  
Martha Grout ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Conjugate Vaccine ◽  
Significant Proportion ◽  
Vaccine Trial ◽  
Keyhole Limpet Hemocyanin ◽  
Opsonic Activity ◽  
Human Sera ◽  
Normal Human

ABSTRACT Deterioration of lung function in patients with cystic fibrosis (CF) is closely associated with chronic pulmonary infection with mucoid Pseudomonas aeruginosa. The mucoid exopolysaccharide (MEP) from P. aeruginosa has been shown to induce opsonic antibodies in mice that are protective against this chronic infection. MEP-specific opsonic antibodies are also commonly found in the sera of older CF patients lacking detectable P. aeruginosa infection. When used in a human vaccine trial, however, MEP only minimally induced opsonic antibodies. To evaluate whether conjugation of MEP to a carrier protein could improve its immunogenicity, we bound thiolated MEP to keyhole limpet hemocyanin (KLH) by using succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) as a linker. In contrast to the native MEP polymer, the MEP-KLH conjugate vaccine induced high titers of MEP-specific immunoglobulin G (IgG) in C3H-HeN mice and in a rabbit. Sera from mice immunized with MEP-KLH conjugate, but not from animals immunized with comparable doses of native MEP, demonstrated opsonic killing activity. Vaccination with MEP-KLH conjugate induced opsonic antibodies broadly cross-reactive to heterologous mucoid strains of P. aeruginosa. Preexisting nonopsonic antibodies to MEP are found in normal human sera, including young CF patients, and their presence impedes the induction of opsonic antibodies. Induction of nonopsonic antibodies by either intraperitoneal injection of MEP or injection or feeding of the cross-reactive antigen, seaweed alginate, reduced the level of overall IgG elicited by follow-up immunization with the MEP-KLH conjugate. However, the opsonic activity was lower only in the sera of MEP-KLH conjugate-immunized mice with preexisting antibodies induced by MEP but not with antibodies induced by seaweed alginate. Immunization with MEP-KLH elicited a significant proportion of antibodies specific to epitopes involving O-acetate residues, and this subpopulation of antibodies mediated opsonic killing of mucoid P. aeruginosa in vitro. These results indicate that conjugation of MEP to KLH significantly enhances its immunogenicity and the elicitation of opsonic antibodies in mice and rabbits, that the conjugate induces opsonic antibodies in the presence of preexisting nonopsonic antibodies, and that opsonic antibodies to MEP are directed at epitopes that include acetate residues on the uronic acid polymer.

scholarly journals Molecular characterization of human anti-hinge antibodies derived from single-cell cloning of normal human B cells

2017 ◽  
Vol 293 (3) ◽  
pp. 906-919 ◽  
Author(s):  
Tao Huang ◽  
Mary Mathieu ◽  
Sophia Lee ◽  
Xinhua Wang ◽  
Yee Seir Kee ◽  
...  
Keyword(s):  
B Cells ◽  
Single Cell ◽  
Molecular Characterization ◽  
Cell Cloning ◽  
Human B Cells ◽  
Single Cell Cloning ◽  
Normal Human
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