Characterization of a Spontaneously Immortalized Cell Line (IOBA-NHC) from Normal Human Conjunctiva

2003 ◽  
Vol 44 (10) ◽  
pp. 4263 ◽  
Author(s):  
Yolanda Diebold ◽  
Margarita Calonge ◽  
Amalia Enri´quez de Salamanca ◽  
Sagrario Callejo ◽  
Rosa M. Corrales ◽  
...  
Keyword(s):  
Cell Line ◽  
Immortalized Cell Line ◽  
Normal Human ◽  
Human Conjunctiva ◽  
Immortalized Cell

Generation and Preliminary Characterization of Immortalized Cell Line Derived from Rat Lymphatic Capillaries

2014 ◽  
Vol 21 (6) ◽  
pp. 551-561 ◽  
Author(s):  
Liudmila G. Romanova ◽  
Eric A. Hansen ◽  
Cornelius H. Lam
Keyword(s):  
Cell Line ◽  
Preliminary Characterization ◽  
Immortalized Cell Line ◽  
Immortalized Cell

scholarly journals Establishment and characterization of BHD-F59RSVT, an immortalized cell line derived from a renal cell carcinoma in a patient with Birt–Hogg–Dubé syndrome

2016 ◽  
Vol 97 (3) ◽  
pp. 343-351 ◽  
Author(s):  
Mitsuko Furuya ◽  
Hisashi Hasumi ◽  
Masaya Baba ◽  
Reiko Tanaka ◽  
Yasuhiro Iribe ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Renal Cell ◽  
Immortalized Cell Line ◽  
Immortalized Cell

scholarly journals Isolation of Primary Mouse Pulmonary Microvascular Endothelial Cells and Generation of an Immortalized Cell Line to Obtain Sufficient Extracellular Vesicles

2021 ◽  
Vol 12 ◽  
Author(s):  
Xu Liu ◽  
Feiping Xia ◽  
Xiao Wu ◽  
Ying Tang ◽  
Lu Wang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Line ◽  
Extracellular Vesicles ◽  
Phenotypic Expression ◽  
Microvascular Endothelial Cells ◽  
Pulmonary Microvascular Endothelial Cells ◽  
Immortalized Cell Line ◽  
Primary Mouse ◽  
Microvascular Endothelial ◽  
Immortalized Cell

Pulmonary microvascular endothelial cells (PMECs) and the extracellular vesicles (EVs) derived from PMECs participate in maintaining pulmonary homeostasis and mediating the inflammatory response. However, obtaining a high-purity population of PMECs and their EVs from mouse is still notoriously difficult. Herein we provide a method to isolate primary mouse PMECs (pMPMECs) and to transduce SV40 lentivirus into pMPMECs to establish an immortalized cell line (iMPMECs), which provides sufficient quantities of EVs for further studies. pMPMECs and iMPMECs can be identified using morphologic criteria, a phenotypic expression profile (e.g., CD31, CD144, G. simplicifolia lectin binding), and functional properties (e.g., Dil-acetylated low-density protein uptake, Matrigel angiogenesis). Furthermore, pMPMEC–EVs and iMPMEC–EVs can be identified and compared. The characteristics of pMPMEC–EVs and iMPMEC–EVs are ascertained by transmission electron microscopy, nanoparticle tracking analysis, and specific protein markers. iMPMECs produce far more EVs than pMPMECs, while their particle size distribution is similar. Our detailed protocol to isolate and immortalize MPMECs will provide researchers with an in vitro model to investigate the specific roles of EVs in pulmonary physiology and diseases.

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