Caspase-3 Is Actively Involved in Okadaic Acid-Induced Lens Epithelial Cell Apoptosis

2001 ◽  
Vol 266 (2) ◽  
pp. 279-291 ◽  
Author(s):  
David Wan-Cheng Li ◽  
Hua Xiang ◽  
Ying-Wei Mao ◽  
Juan Wang ◽  
Uwe Fass ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Okadaic Acid ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Lens Epithelial Cell ◽  
Epithelial Cell Apoptosis

TUG1 promotes lens epithelial cell apoptosis by regulating miR-421/caspase-3 axis in age-related cataract

2017 ◽  
Vol 356 (1) ◽  
pp. 20-27 ◽  
Author(s):  
Guoxing Li ◽  
Huiyang Song ◽  
Lei Chen ◽  
Weihua Yang ◽  
Kaihui Nan ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Lens Epithelial Cell ◽  
Age Related ◽  
Epithelial Cell Apoptosis

Down-regulation of GJA3 is associated with lens epithelial cell apoptosis and age-related cataract

2017 ◽  
Vol 484 (1) ◽  
pp. 159-164 ◽  
Author(s):  
Dongmei Su ◽  
Shanshan Hu ◽  
Lina Guan ◽  
Xinzhu Wu ◽  
Cuige Shi ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Apoptosis ◽  
Lens Epithelial Cell ◽  
Down Regulation ◽  
Age Related ◽  
Epithelial Cell Apoptosis

siRNA Interference with Notch1 Affects LPS-Induced Apoptosis of Rat Intestinal Epithelial Cells

2020 ◽  
Vol 10 (5) ◽  
pp. 640-646
Author(s):  
Jing Li ◽  
Guanming Ruan ◽  
Xiaoyan Hu ◽  
Juhui He
Keyword(s):  
Epithelial Cell ◽  
Notch Signaling ◽  
Cell Apoptosis ◽  
Intestinal Epithelial Cell ◽  
Caspase 3 ◽  
Control Group ◽  
Ros Production ◽  
Intestinal Epithelial ◽  
Colon Tissue ◽  
Epithelial Cell Apoptosis

Notch signaling is associated with the pathogenesis of ulcerative colitis (UC). This study investigated whether the Notch regulates intestinal epithelial cell apoptosis and reactive oxygen species (ROS) production. Rats were separated into UC model group and control group followed by analysis of the expression of Notch1, Hes1 and OLFM4 by real-time PCR and western blot, content of malondialdehyde (MDA), superoxide dismutase (SOD) and caspase-3 as well as cell apoptosis by flow cytometry. Under LPS stimulation conditions, IEC-6 cells were divided into LPS + siRNA-NC group and LPS + siRNA-Notch1 group and Notch1, Hes1 and OLFM4 level as well as ROS was measured. Compared with control group, the activity of caspase-3, MDA and apoptosis in colon tissue of UC model were significantly increased with decreased SOD activity and increased Notch1, Hes1 and OLFM4 expression. LPS treatment can significantly up-regulate the expression of Notch1, Hes1 and OLFM4 in IEC-6 cells, increase cell apoptosis and ROS production. Notch1 interference by siRNA significantly downregulated Notch1, Hes1, and OLFM4 in IEC cells, and increased cell apoptosis and ROS production. UC lesions can activate Notch signaling pathway in colon tissue, which may play a role in repair. Interference with Notch1 signaling may aggravate intestinal epithelial cell apoptosis and ROS production in an inflammatory environment.

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