Fibroblast Growth Factor-1 Induction of Delayed-Early mRNA Expression in NIH 3T3 Cells Is Prolonged by Heparin Addition

1997 ◽  
Vol 234 (1) ◽  
pp. 139-146 ◽  
Author(s):  
Patrick J. Donohue ◽  
Debbie K.W. Hsu ◽  
Yan Guo ◽  
Wilson H. Burgess ◽  
Jeffrey A. Winkles
Keyword(s):  
Growth Factor ◽  
Mrna Expression ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
3T3 Cells ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

scholarly journals Fibroblast growth factor-1 stimulation of quiescent NIH 3T3 cells increases G/T mismatch-binding protein expression

1996 ◽  
Vol 319 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Patrick J. DONOHUE ◽  
Sheau-Line Y. FENG ◽  
Gregory F ALBERTS ◽  
Yan GUO ◽  
Kimberly A PEIFLEY ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Binding Protein ◽  
Calf Serum ◽  
Mrna Levels ◽  
Fibroblast Growth ◽  
3T3 Cells ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

Polypeptide growth factors promote cell-cycle progression in part by the transcriptional activation of a diverse group of specific genes. We have used an mRNA differential-display approach to identify several fibroblast growth factor (FGF)-1 (acidic FGF)-inducible genes in NIH 3T3 cells. Here we report that one of these genes, called FGF-regulated (FR)-3, is predicted to encode G/T mismatch-binding protein (GTBP), a component of the mammalian DNA mismatch correction system. The murine GTBP gene is transiently expressed after FGF-1 or calf serum treatment, with maximal mRNA levels detected at 12 and 18 h post-stimulation. FGF-1-stimulated NIH 3T3 cells also express an increased amount of GTBP as determined by immunoblot analysis. These results indicate that elevated levels of GTBP may be required during the DNA synthesis phase of the cell cycle for efficient G/T mismatch recognition and repair.

scholarly journals Selective expression of high molecular weight basic fibroblast growth factor confers a unique phenotype to NIH 3T3 cells.

1991 ◽  
Vol 2 (9) ◽  
pp. 699-708 ◽  
Author(s):  
N Quarto ◽  
D Talarico ◽  
R Florkiewicz ◽  
D B Rifkin
Keyword(s):  
Molecular Weight ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
High Molecular Weight ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
3T3 Cells ◽  
Low Levels ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

The phenotypes of NIH 3T3 cells transfected with basic fibroblast growth factor (bFGF) cDNAs that express only the high molecular weight (HMW) forms of bFGF, the 18-kDa form, or all forms were examined. Cells producing the 18 kDa or all forms of bFGF were transformed at high levels of growth factor expression but were nontransformed at low levels. Cell producing low levels of HMW forms of bFGF were growth impaired when compared with the parental cells. These cells tended to form multinucleated giant cells, did not grow in soft agar, were nontumorigenic, had a normal bFGF receptor number, and had a nontransformed morphology. Cells expressing high levels of HMW bFGFs had a transformed morphology and were tumorigenic. These data suggest a specific functional role for HMWbFGF.

scholarly journals The Release of Fibroblast Growth Factor-1 from NIH 3T3 Cells in Response to Temperature Involves the Function of Cysteine Residues

1995 ◽  
Vol 270 (1) ◽  
pp. 33-36 ◽  
Author(s):  
Anthony Jackson ◽  
Francesca Tarantini ◽  
Susan Gamble ◽  
Stanley Friedman ◽  
Thomas Maciag
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Cysteine Residues ◽  
Fibroblast Growth ◽  
3T3 Cells ◽  
Response To Temperature ◽  
Nih 3T3 ◽  
Nih 3T3 Cells
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