Transformed phenotype conferred to NIH/3T3 cells by ectopic expression of heparin-binding growth factor 1/acidic fibroblast growth factor

1991 ◽  
Vol 27 (1) ◽  
pp. 89-96 ◽  
Author(s):  
Phanpimol Bunnag ◽  
Karen S. Waddell ◽  
M. Lee Varban ◽  
Ing-Ming Chiu
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Ectopic Expression ◽  
Fibroblast Growth ◽  
Heparin Binding ◽  
3T3 Cells ◽  
Acidic Fibroblast Growth Factor ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

scholarly journals Fibroblast growth factor-1 stimulation of quiescent NIH 3T3 cells increases G/T mismatch-binding protein expression

1996 ◽  
Vol 319 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Patrick J. DONOHUE ◽  
Sheau-Line Y. FENG ◽  
Gregory F ALBERTS ◽  
Yan GUO ◽  
Kimberly A PEIFLEY ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Binding Protein ◽  
Calf Serum ◽  
Mrna Levels ◽  
Fibroblast Growth ◽  
3T3 Cells ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

Polypeptide growth factors promote cell-cycle progression in part by the transcriptional activation of a diverse group of specific genes. We have used an mRNA differential-display approach to identify several fibroblast growth factor (FGF)-1 (acidic FGF)-inducible genes in NIH 3T3 cells. Here we report that one of these genes, called FGF-regulated (FR)-3, is predicted to encode G/T mismatch-binding protein (GTBP), a component of the mammalian DNA mismatch correction system. The murine GTBP gene is transiently expressed after FGF-1 or calf serum treatment, with maximal mRNA levels detected at 12 and 18 h post-stimulation. FGF-1-stimulated NIH 3T3 cells also express an increased amount of GTBP as determined by immunoblot analysis. These results indicate that elevated levels of GTBP may be required during the DNA synthesis phase of the cell cycle for efficient G/T mismatch recognition and repair.

scholarly journals Selective expression of high molecular weight basic fibroblast growth factor confers a unique phenotype to NIH 3T3 cells.

1991 ◽  
Vol 2 (9) ◽  
pp. 699-708 ◽  
Author(s):  
N Quarto ◽  
D Talarico ◽  
R Florkiewicz ◽  
D B Rifkin
Keyword(s):  
Molecular Weight ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
High Molecular Weight ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
3T3 Cells ◽  
Low Levels ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

The phenotypes of NIH 3T3 cells transfected with basic fibroblast growth factor (bFGF) cDNAs that express only the high molecular weight (HMW) forms of bFGF, the 18-kDa form, or all forms were examined. Cells producing the 18 kDa or all forms of bFGF were transformed at high levels of growth factor expression but were nontransformed at low levels. Cell producing low levels of HMW forms of bFGF were growth impaired when compared with the parental cells. These cells tended to form multinucleated giant cells, did not grow in soft agar, were nontumorigenic, had a normal bFGF receptor number, and had a nontransformed morphology. Cells expressing high levels of HMW bFGFs had a transformed morphology and were tumorigenic. These data suggest a specific functional role for HMWbFGF.

scholarly journals The Release of Fibroblast Growth Factor-1 from NIH 3T3 Cells in Response to Temperature Involves the Function of Cysteine Residues

1995 ◽  
Vol 270 (1) ◽  
pp. 33-36 ◽  
Author(s):  
Anthony Jackson ◽  
Francesca Tarantini ◽  
Susan Gamble ◽  
Stanley Friedman ◽  
Thomas Maciag
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Cysteine Residues ◽  
Fibroblast Growth ◽  
3T3 Cells ◽  
Response To Temperature ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

scholarly journals Isolation and characterization of a macrophage-derived heparin-binding growth factor.

1990 ◽  
Vol 1 (11) ◽  
pp. 811-819 ◽  
Author(s):  
G Besner ◽  
S Higashiyama ◽  
M Klagsbrun
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Mononuclear Cells ◽  
Vascular Endothelial Cells ◽  
Fibroblast Growth ◽  
Heparin Binding ◽  
3T3 Cells ◽  
Acidic Fibroblast Growth Factor ◽  
Western Blots ◽  
Isolation And Characterization

Human mononuclear cells were plated in culture, and the conditioned media of these cells were analyzed by heparin-Sepharose affinity chromatography. The fractions were tested for growth factor activity as measured by the stimulation of DNA synthesis in BALB/c 3T3 cells. After 2 d in culture, two peaks of heparin-binding growth factor (HBGF) activity were detected, one eluting with 0.5 M NaCl, which could be shown to be platelet-derived growth factor (PDGF)-like, and the other eluting with 1.0 M NaCl. After 7-11 d in culture, when monocytes had clearly differentiated into macrophages, greater than 95% of the HBGF activity in conditioned medium consisted of the 1.0 M NaCl elution peak. This activity, which was designated macrophage-derived HBGF (MD-HBGF), was found to be a cationic heat-resistant polypeptide with a molecular weight in the range of 14-25 kDa. Analysis using Western blots and specific neutralizing antisera, as well as comparative heparin affinity analysis, indicated that MD-HBGF was not identical to other heparin-binding 3T3 cell growth factors known to be produced by macrophages, such as PDGF (AB, AA, and BB forms), acidic fibroblast growth factor, and basic fibroblast growth factor. In addition to stimulating mitogenesis in 3T3 cells, MD-HBGF also stimulated the proliferation of vascular smooth muscle cells, but did not stimulate the proliferation of vascular endothelial cells.

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