scholarly journals Efficient Gene Transfer into the Embryonic Mouse Brain Using in Vivo Electroporation

2001 ◽  
Vol 240 (1) ◽  
pp. 237-246 ◽  
Author(s):  
Tetsuichiro Saito ◽  
Norio Nakatsuji
Keyword(s):  
Gene Transfer ◽  
Mouse Brain ◽  
In Vivo Electroporation ◽  
Embryonic Mouse ◽  
Efficient Gene

In vivo electroporation enhances plasmid-based gene transfer of basic fibroblast growth factor for the treatment of ischemic limb

2004 ◽  
Vol 120 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Seiji Nishikage ◽  
Hiroyuki Koyama ◽  
Tetsuro Miyata ◽  
Shigeyuki Ishii ◽  
Hirohumi Hamada ◽  
...  
Keyword(s):  
Growth Factor ◽  
Gene Transfer ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
In Vivo Electroporation ◽  
Ischemic Limb

Efficient gene delivery into murine ovarian cells by intraovarian injection of plasmid DNA and subsequent in vivo electroporation

genesis ◽  
2003 ◽  
Vol 35 (3) ◽  
pp. 169-174 ◽  
Author(s):  
Masahiro Sato ◽  
Maya Tanigawa ◽  
Natsuko Kikuchi ◽  
Shingo Nakamura ◽  
Minoru Kimura
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna ◽  
In Vivo Electroporation ◽  
Ovarian Cells ◽  
Efficient Gene

scholarly journals Local Gene Delivery System by Bubble Liposomes and Ultrasound Exposure into Joint Synovium

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Yoichi Negishi ◽  
Yuka Tsunoda ◽  
Yoko Endo-Takahashi ◽  
Yusuke Oda ◽  
Ryo Suzuki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Gene Delivery ◽  
Delivery System ◽  
Imaging System ◽  
Effective Means ◽  
Gene Delivery System ◽  
Highly Efficient ◽  
Efficient Gene

Recently, we have developed novel polyethylene glycol modified liposomes (bubble liposomes; BL) entrapping an ultrasound (US) imaging gas, which can work as a gene delivery tool with US exposure. In this study, we investigated the usefulness of US-mediated gene transfer systems with BL into synoviocytes in vitro and joint synovium in vivo. Highly efficient gene transfer could be achieved in the cultured primary synoviocytes transfected with the combination of BL and US exposure, compared to treatment with plasmid DNA (pDNA) alone, pDNA plus BL, or pDNA plus US. When BL was injected into the knee joints of mice, and US exposure was applied transcutaneously to the injection site, highly efficient gene expression could be observed in the knee joint transfected with the combination of BL and US exposure, compared to treatment with pDNA alone, pDNA plus BL, or pDNA plus US. The localized and prolonged gene expression was also shown by an in vivo luciferase imaging system. Thus, this local gene delivery system into joint synovium using the combination of BL and US exposure may be an effective means for gene therapy in joint disorders.

Direct gene transfer into rat articular cartilage by in vivo electroporation

2003 ◽  
Vol 17 (8) ◽  
pp. 829-835 ◽  
Author(s):  
Lagrent Grossin ◽  
Christel Cournil-Henrionnet ◽  
Lluis M. Mir ◽  
Bertrand Liagre ◽  
Dominique Dumas ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Gene Transfer ◽  
Direct Gene Transfer ◽  
In Vivo Electroporation ◽  
Direct Gene

scholarly journals Ovine Adenovirus Vectors Overcome Preexisting Humoral Immunity against Human Adenoviruses In Vivo

1999 ◽  
Vol 73 (8) ◽  
pp. 6930-6936 ◽  
Author(s):  
Christian Hofmann ◽  
Peter Löser ◽  
Günter Cichon ◽  
Wolfgang Arnold ◽  
Gerald W. Both ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Humoral Immunity ◽  
Functional Expression ◽  
Immunodeficient Mice ◽  
Human Adenoviruses ◽  
Rous Sarcoma ◽  
Efficient Gene ◽  
Human Sera ◽  
Adenovirus Vectors

ABSTRACT Recombinant human adenoviruses (hAd) have become widely used as tools to achieve efficient gene transfer. However, successful application of hAd-derived vectors in clinical trials is limited due to immunological and potential safety problems inherent in their human origin. In this study, we describe a recombinant ovine adenovirus (OAV) as an alternative vector for gene transfer in vivo. In contrast to an hAd vector, the OAV vector was not neutralized by human sera. An OAV vector which contained the cDNA of the human α1-antitrypsin (hAAT) gene linked to the Rous sarcoma virus promoter was generated and administered systemically to mice. The level and duration of hAAT gene expression was similar to that achieved with an hAd counterpart in both immunocompetent and immunodeficient mice. However, the tissue distribution of the OAV vector differed from that observed for hAd vectors in that the liver was not the dominant target. Significantly, we demonstrated efficient gene transfer with the OAV vector into mice immunized with hAd vectors and vice versa. We also confirm that the immune response to a transgene product can prevent its functional expression following sequential application of a vector. Our results suggest a possible solution to endemic humoral immunity against currently used hAd vectors and should therefore have an impact on the design of improved gene therapy protocols utilizing adenovirus vectors.

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