scholarly journals Local Gene Delivery System by Bubble Liposomes and Ultrasound Exposure into Joint Synovium

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Yoichi Negishi ◽  
Yuka Tsunoda ◽  
Yoko Endo-Takahashi ◽  
Yusuke Oda ◽  
Ryo Suzuki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Gene Delivery ◽  
Delivery System ◽  
Imaging System ◽  
Effective Means ◽  
Gene Delivery System ◽  
Highly Efficient ◽  
Efficient Gene

Recently, we have developed novel polyethylene glycol modified liposomes (bubble liposomes; BL) entrapping an ultrasound (US) imaging gas, which can work as a gene delivery tool with US exposure. In this study, we investigated the usefulness of US-mediated gene transfer systems with BL into synoviocytes in vitro and joint synovium in vivo. Highly efficient gene transfer could be achieved in the cultured primary synoviocytes transfected with the combination of BL and US exposure, compared to treatment with plasmid DNA (pDNA) alone, pDNA plus BL, or pDNA plus US. When BL was injected into the knee joints of mice, and US exposure was applied transcutaneously to the injection site, highly efficient gene expression could be observed in the knee joint transfected with the combination of BL and US exposure, compared to treatment with pDNA alone, pDNA plus BL, or pDNA plus US. The localized and prolonged gene expression was also shown by an in vivo luciferase imaging system. Thus, this local gene delivery system into joint synovium using the combination of BL and US exposure may be an effective means for gene therapy in joint disorders.

The Combination of Drug or Gene Delivery System Responding to Cellular Signals (D-RECS) and Sonoporation System for Effective and Safe Gene Delivery

2009 ◽  
Vol 1237 ◽  
Author(s):  
Akira Tsuchiya ◽  
Takeshi Mori ◽  
Yuki Naritomi ◽  
Jeong-Hun Kang ◽  
Daisuke Asai ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Regulation ◽  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Regulation System ◽  
Gene Delivery System

AbstractWe have developed new gene expression-regulating polymer that can activate transgene expression in response to target intracellular signals. Here, we tried applying sonoporation system to this gene regulation system to enhance the gene expression efficacy. Sonoporation is the method for effective gene transfection in vitro and in vivo. Therefore, the method might enhance the transfection efficiency in our polymer and realize an efficient and safe gene delivery system. Results suggested that the combination of our polymer and sonoporation could improve the gene expression compared to the system using only our polymer that transfers genes into cells via endocytosis. It also kept the ability of the gene regulation responding to cellular signals.

A progressively targeted gene delivery system with a pH triggered surface charge-switching ability to drive angiogenesis in vivo

2019 ◽  
Vol 7 (5) ◽  
pp. 2061-2075 ◽  
Author(s):  
Bin Gao ◽  
Qiaoping Zhang ◽  
Khan Muhammad ◽  
Xiangkui Ren ◽  
Jintang Guo ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Surface Charge ◽  
Delivery System ◽  
Gene Delivery System ◽  
Targeted Gene Delivery ◽  
Highly Efficient ◽  
Ability To Drive

A progressively targeted ternary gene delivery system with charge-switching ability for achieving highly efficient angiogenesis in vivo.

Poly(ethylene glycol)-poly-l-glutamate complexed with polyethyleneimine−polyglycine for highly efficient gene delivery in vitro and in vivo

2018 ◽  
Vol 6 (11) ◽  
pp. 3053-3062 ◽  
Author(s):  
Zhaopei Guo ◽  
Lin Lin ◽  
Jie Chen ◽  
Xingzhi Zhou ◽  
Hon Fai Chan ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Gene Delivery ◽  
Ethylene Glycol ◽  
Gene Delivery System ◽  
Poly Ethylene Glycol ◽  
Highly Efficient ◽  
Efficient Gene ◽  
Poly Ethylene

The highly efficient gene delivery system with effective serum resistant capacity is promising for cancer therapy.

1004: In-Vivo Transfection of the Rat Corpus Cavernosum Using a Non-Viral, Linear Polyethylenimine Gene Delivery System: A Novel Application

2006 ◽  
Vol 175 (4S) ◽  
pp. 323-324 ◽  
Author(s):  
Joseph Dall'era ◽  
Sweaty Koul ◽  
Jesse Mills ◽  
Jeremy Myers ◽  
Randall B. Meacham ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Corpus Cavernosum ◽  
Gene Delivery System ◽  
System A ◽  
In Vivo Transfection ◽  
Linear Polyethylenimine

Polyplex: A Promising Gene Delivery System

2019 ◽  
Vol 12 (6) ◽  
pp. 4681-4686
Author(s):  
Rohan Aggarwal ◽  
Monika Targhotra ◽  
Bhumika Kumar ◽  
P.K Sahoo ◽  
Meenakshi K Chauhan
Keyword(s):  
Gene Transfer ◽  
Gene Delivery ◽  
Delivery System ◽  
Viral Vectors ◽  
Gene Delivery System ◽  
Cationic Polymers ◽  
Therapeutic Application ◽  
Promising Alternative ◽  
Structure And Stability ◽  
The Past

In the past few years gene delivery system has gained a huge attention owing to its proved efficacy in several diseases especially in those caused by genetic and/oroncological malfunctioning. The effective gene delivery mainly depends on the carrier molecules that can ensure the safe and specific delivery of the nucleic acidmolecules. Viral vectors have been used for a longer period as the gene transfer vehicle. However, these viral vectors have potential immunological disadvantages that made them less preferred. Recently, non-viral vectors such as polyplexes have emerged as a promising alternative for viral vectors. Polyplexes are formed by conjugating a polymer with DNA and in maximum cases the cationic polymers are preferred over others. The structure and stability of the polyplexes depends on various factors. The ability of the polymer to condense the DNA mainly dictates the efficiency of the polyplex mediated transfection. In this review we are going to provide a framework for the synthesis and design of the polyplexes along with the structure and stability of the complexes pertaining to mechanism of action, characterization and therapeutic application, including polyethyleneimine mediated cytotoxicity as well as newer strategies for the generation of better polyplexes.

In vivo uterine local gene delivery system using TAT‐displaying bionanocapsules

2019 ◽  
Vol 21 (12) ◽  
Author(s):  
Kaori Koizumi ◽  
Hitomi Nakamura ◽  
Masumi Iijima ◽  
Takashi Matsuzaki ◽  
Masaharu Somiya ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Gene Delivery System

From single to a dual-gene delivery nanosystem: coordinated expression matters for boosting the neovascularization in vivo

2020 ◽  
Vol 8 (8) ◽  
pp. 2318-2328 ◽  
Author(s):  
Bin Gao ◽  
Xiaoyu Wang ◽  
Meiyu Wang ◽  
Xiang-kui Ren ◽  
Jintang Guo ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Gene Delivery System ◽  
Coordinated Expression

A dual-gene delivery system with coordinated expression function boosted the neovascularization.

scholarly journals Echogenic PEGylated PEI-Loaded Microbubble As Efficient Gene Delivery System

2019 ◽  
Vol Volume 14 ◽  
pp. 8923-8941 ◽  
Author(s):  
Chun Liufu ◽  
Yue Li ◽  
Jiawei Tu ◽  
Hui Zhang ◽  
Jinsui Yu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Gene Delivery System ◽  
System P ◽  
Efficient Gene
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