scholarly journals Expression Cloning of Xenopus Os4, an Evolutionarily Conserved Gene, which Induces Mesoderm and Dorsal Axis

2001 ◽  
Vol 239 (1) ◽  
pp. 118-131 ◽  
Author(s):  
Irene E. Zohn ◽  
Ali H. Brivanlou
Keyword(s):  
Expression Cloning ◽  
Evolutionarily Conserved ◽  
Dorsal Axis ◽  
Conserved Gene

scholarly journals Vip1 is a kinase and pyrophosphatase switch that regulates inositol diphosphate signaling

2020 ◽  
Vol 117 (17) ◽  
pp. 9356-9364 ◽  
Author(s):  
D. Eric Dollins ◽  
Wenli Bai ◽  
Peter C. Fridy ◽  
James C. Otto ◽  
Julie L. Neubauer ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Gene Product ◽  
Active Sites ◽  
High Energy ◽  
Atomic Resolution ◽  
Cellular Adaptation ◽  
Dual Functional ◽  
Evolutionarily Conserved ◽  
Inositol Pyrophosphates ◽  
Conserved Gene

Inositol diphosphates (PP-IPs), also known as inositol pyrophosphates, are high-energy cellular signaling codes involved in nutrient and regulatory responses. We report that the evolutionarily conserved gene product, Vip1, possesses autonomous kinase and pyrophosphatase domains capable of synthesis and destruction of D-1 PP-IPs. Our studies provide atomic-resolution structures of the PP-IP products and unequivocally define that the Vip1 gene product is a highly selective 1-kinase and 1-pyrophosphatase enzyme whose activities arise through distinct active sites. Kinetic analyses of kinase and pyrophosphatase parameters are consistent with Vip1 evolving to modulate levels of 1-IP7 and 1,5-IP8. Individual perturbations in kinase and pyrophosphatase activities in cells result in differential effects on vacuolar morphology and osmotic responses. Analogous to the dual-functional key energy metabolism regulator, phosphofructokinase 2, Vip1 is a kinase and pyrophosphatase switch whose 1-PP-IP products play an important role in a cellular adaptation.

scholarly journals An evolutionarily conserved gene family encodes proton-selective ion channels

Science ◽  
2018 ◽  
Vol 359 (6379) ◽  
pp. 1047-1050 ◽  
Author(s):  
Yu-Hsiang Tu ◽  
Alexander J. Cooper ◽  
Bochuan Teng ◽  
Rui B. Chang ◽  
Daniel J. Artiga ◽  
...  
Keyword(s):  
Ion Channels ◽  
Gene Family ◽  
Evolutionarily Conserved ◽  
Conserved Gene

scholarly journals MHO1, an Evolutionarily Conserved Gene, Is Synthetic Lethal with PLC1; Mho1p Has a Role in Invasive Growth

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e32501 ◽  
Author(s):  
Ivan D. Schlatter ◽  
Maria Meira ◽  
Vanessa Ueberschlag ◽  
Dominic Hoepfner ◽  
Rao Movva ◽  
...  
Keyword(s):  
Invasive Growth ◽  
Synthetic Lethal ◽  
Evolutionarily Conserved ◽  
Conserved Gene

scholarly journals Zygote Arrest 1 (Zar1) Is an Evolutionarily Conserved Gene Expressed in Vertebrate Ovaries1

2003 ◽  
Vol 69 (3) ◽  
pp. 861-867 ◽  
Author(s):  
Xuemei Wu ◽  
Pei Wang ◽  
Christopher A. Brown ◽  
Carolyn A. Zilinski ◽  
Martin M. Matzuk
Keyword(s):  
Evolutionarily Conserved ◽  
Conserved Gene

scholarly journals Wnt/β-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Iryna Kozmikova ◽  
Zbynek Kozmik
Keyword(s):  
Cell Fate ◽  
Current View ◽  
Specific Gene ◽  
Axis Formation ◽  
Dependent Manner ◽  
Evolutionarily Conserved ◽  
Dorsal Axis ◽  
Dorsal Cells ◽  
Dorsal Cell Fate

Deciphering the mechanisms of axis formation in amphioxus is a key step to understanding the evolution of chordate body plan. The current view is that Nodal signaling is the only factor promoting the dorsal axis specification in the amphioxus, whereas Wnt/β-catenin signaling plays no role in this process. Here, we re-examined the role of Wnt/βcatenin signaling in the dorsal/ventral patterning of amphioxus embryo. We demonstrated that the spatial activity of Wnt/β-catenin signaling is located in presumptive dorsal cells from cleavage to gastrula stage, and provided functional evidence that Wnt/β-catenin signaling is necessary for the specification of dorsal cell fate in a stage-dependent manner. Microinjection of Wnt8 and Wnt11 mRNA induced ectopic dorsal axis in neurulae and larvae. Finally, we demonstrated that Nodal and Wnt/β-catenin signaling cooperate to promote the dorsal-specific gene expression in amphioxus gastrula. Our study reveals high evolutionary conservation of dorsal organizer formation in the chordate lineage.

The Xenopus homeobox gene twin mediates Wnt induction of goosecoid in establishment of Spemann's organizer

Development ◽  
1997 ◽  
Vol 124 (23) ◽  
pp. 4905-4916 ◽  
Author(s):  
M.N. Laurent ◽  
I.L. Blitz ◽  
C. Hashimoto ◽  
U. Rothbacher ◽  
K.W. Cho
Keyword(s):  
Reporter Gene ◽  
Binding Sites ◽  
Homeobox Gene ◽  
Dorsal Side ◽  
Expression Cloning ◽  
Specific Gene ◽  
Dependent Manner ◽  
Spemann's Organizer ◽  
Dorsal Axis

We describe the isolation of the Xenopus homeobox gene twin (Xtwn), which was identified in an expression cloning screen for molecules with dorsalizing activities. Injection of synthetic Xtwn mRNA restores a complete dorsal axis in embryos lacking dorsal structures and induces a complete secondary dorsal axis when ectopically expressed in normal embryos. The sequence homology, expression pattern and gain-of-function phenotype of Xtwn is most similar to the previously isolated Xenopus homeobox gene siamois (Xsia) suggesting that Xtwn and Xsia comprise a new subclass of homeobox genes important in dorsal axis specification. We find that Xtwn is able to activate the Spemann organizer-specific gene goosecoid (gsc) via direct binding to a region of the gsc promoter previously shown to mediate Wnt induction. Since Xtwn expression is strongly induced in ectodermal (animal cap) cells in response to overexpression of a dorsalizing Wnt molecule, we examined the possibility that Xtwn might be a direct target of a Wnt signal transduction cascade. First, we demonstrate that purified LEF1 protein can interact, in vitro, with consensus LEF1/TCF3-binding sites found within the Xtwn promoter. Second, these binding sites were shown to be required for Wnt-mediated induction of a Xtwn reporter gene containing these sites. As LEF1/TCF3 family transcription factors have previously been shown to directly mediate Wnt signaling, these results suggest that Xtwn induction by Wnt may be direct. Finally, in UV-hyperventralized embryos, expression of endogenous Xtwn is confined to the vegetal pole and a Xtwn reporter gene is hyperinduced vegetally in a LEF1/TCF3-binding-site-dependent manner. These results suggest that cortical rotation distributes Wnt-like dorsal determinants to the dorsal side of the embryo, including the dorsal marginal zone, and that these determinants may directly establish Spemann's organizer in this region.

scholarly journals Chromosomal periodicity of evolutionarily conserved gene pairs

2007 ◽  
Vol 104 (25) ◽  
pp. 10559-10564 ◽  
Author(s):  
M. A. Wright ◽  
P. Kharchenko ◽  
G. M. Church ◽  
D. Segre
Keyword(s):  
Gene Pairs ◽  
Evolutionarily Conserved ◽  
Conserved Gene
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