scholarly journals POU Domain Factor Brn-3b Is Essential for Retinal Ganglion Cell Differentiation and Survival but Not for Initial Cell Fate Specification

1999 ◽  
Vol 210 (2) ◽  
pp. 469-480 ◽  
Author(s):  
Lin Gan ◽  
Steven W. Wang ◽  
Zhang Huang ◽  
William H. Klein
Keyword(s):  
Cell Differentiation ◽  
Ganglion Cell ◽  
Cell Fate ◽  
Retinal Ganglion Cell ◽  
Retinal Ganglion ◽  
Cell Fate Specification ◽  
Initial Cell ◽  
Fate Specification ◽  
Pou Domain

scholarly journals Zac1 promotes a Müller glial cell fate and interferes with retinal ganglion cell differentiation inXenopus retina

2006 ◽  
Vol 236 (1) ◽  
pp. 192-202 ◽  
Author(s):  
Lin Ma ◽  
Jennifer C. Hocking ◽  
Carrie L. Hehr ◽  
Carol Schuurmans ◽  
Sarah McFarlane
Keyword(s):  
Cell Differentiation ◽  
Ganglion Cell ◽  
Glial Cell ◽  
Cell Fate ◽  
Retinal Ganglion Cell ◽  
Retinal Ganglion ◽  
Müller Glial Cell

scholarly journals Protection by an Oral Disubstituted Hydroxylamine Derivative against Loss of Retinal Ganglion Cell Differentiation following Optic Nerve Crush

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e65966 ◽  
Author(s):  
James D. Lindsey ◽  
Karen X. Duong-Polk ◽  
Yi Dai ◽  
Duy H. Nguyen ◽  
Christopher K. Leung ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Optic Nerve ◽  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Optic Nerve Crush ◽  
Retinal Ganglion ◽  
Nerve Crush ◽  
Hydroxylamine Derivative

All Brn3 genes can promote retinal ganglion cell differentiation in the chick

Development ◽  
2000 ◽  
Vol 127 (15) ◽  
pp. 3237-3247 ◽  
Author(s):  
W. Liu ◽  
S.L. Khare ◽  
X. Liang ◽  
M.A. Peters ◽  
X. Liu ◽  
...  
Keyword(s):  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Ganglion Cells ◽  
Cell Development ◽  
Large Set ◽  
Retinal Ganglion ◽  
Pou Domain ◽  
Temporal Expression Pattern ◽  
Cell Genesis

Targeted gene disruption studies in the mouse have demonstrated crucial roles for the Brn3 POU domain transcription factor genes, Brn3a, Brn3b, Brn3c (now called Pou4f1, Pou4f2, Pou4f3, respectively) in sensorineural development and survival. During mouse retinogenesis, the Brn3b gene is expressed in a large set of postmitotic ganglion cell precursors and is required for their early and terminal differentiation. In contrast, the Brn3a and Brn3c genes, which are expressed later in ganglion cells, appear to be dispensable for ganglion cell development. To understand the mechanism that causes the functional differences of Brn3 genes in retinal development, we employed a gain-of-function approach in the chick embryo. We find that Brn3b(l) and Brn3b(s), the two isoforms encoded by the Brn3b gene, as well as Brn3a and Brn3c all have similar DNA-binding and transactivating activities. We further find that the POU domain is minimally required for these activities. Consequently, we show that all these Brn3 proteins have a similar ability to promote development of ganglion cells when ectopically expressed in retinal progenitors. During chick retinogenesis, cBrn3c instead of cBrn3b exhibits a spatial and temporal expression pattern characteristic of ganglion cell genesis and its misexpression can also increase ganglion cell production. Based on these data, we propose that all Brn3 factors are capable of promoting retinal ganglion cell development, and that this potential may be limited by the order of expression in vivo.

scholarly journals Retinal Stem Cells Transplanted into Models of Late Stages of Retinitis Pigmentosa Preferentially Adopt a Glial or a Retinal Ganglion Cell Fate

2007 ◽  
Vol 48 (1) ◽  
pp. 446 ◽  
Author(s):  
Kriss Canola ◽  
Brigitte Ange´nieux ◽  
Meriem Tekaya ◽  
Alexander Quiambao ◽  
Muna I. Naash ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinitis Pigmentosa ◽  
Ganglion Cell ◽  
Cell Fate ◽  
Retinal Ganglion Cell ◽  
Retinal Ganglion ◽  
Late Stages ◽  
Retinal Stem Cells

scholarly journals Transcriptional logic of cell fate specification and axon guidance in early born retinal neurons revealed by single-cell mRNA profiling

2018 ◽  
Author(s):  
Quentin Lo Giudice ◽  
Marion Leleu ◽  
Pierre J. Fabre
Keyword(s):  
Axon Guidance ◽  
Cell Fate ◽  
Ganglion Cells ◽  
Amacrine Cells ◽  
Retinal Ganglion ◽  
Cell Fate Specification ◽  
Retinal Neurons ◽  
Fate Specification ◽  
Guidance Cues ◽  
Insight Into

ABSTRACTRetinal ganglion cells (RGC), together with cone photoreceptors, horizontal cells (HC) and amacrine cells (AC), are the first classes of neurons produced in the retina. Here we have profiled 5348 single retinal cells and provided a comprehensive transcriptomic atlas showing the broad diversity of the developing retina at the time when the four early-born cells are being produced. Our results show the transcriptional sequences that establish the hierarchical ordering of early cell fate specification in the retina. RGC maturation follows six waves of gene expression, giving new insight into the regulatory logic of RGC differentiation. Early-generated RGCs transcribe an increasing amount of guidance cues for young peripheral RGC axons that express the matching receptors. Finally, spatial signatures in sub-populations of RGCs allowed to define novel molecular markers that are spatially restricted during the development of the retina. Altogether this study is a valuable resource that identifies new players in mouse retinal development, shedding light on transcription factors sequence and guidance cues dynamics in space and time.

scholarly journals Methods of Retinal Ganglion Cell Differentiation From Pluripotent Stem Cells

2014 ◽  
Vol 3 (3) ◽  
pp. 7 ◽  
Author(s):  
Katherine P. Gill ◽  
Alex W. Hewitt ◽  
Kathryn C. Davidson ◽  
Alice Pébay ◽  
Raymond C. B. Wong
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Pluripotent Stem Cells ◽  
Retinal Ganglion

Brn3b/Brn3c double knockout mice reveal an unsuspected role for Brn3c in retinal ganglion cell axon outgrowth

Development ◽  
2002 ◽  
Vol 129 (2) ◽  
pp. 467-477 ◽  
Author(s):  
Steven W. Wang ◽  
Xiuqian Mu ◽  
William J. Bowers ◽  
Dong-Seob Kim ◽  
Daniel J. Plas ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Ganglion Cell ◽  
Retinal Ganglion Cells ◽  
Retinal Ganglion Cell ◽  
Knockout Mice ◽  
Ganglion Cells ◽  
Axon Outgrowth ◽  
Retinal Ganglion ◽  
Double Knockout ◽  
Ganglion Cell Axon

In mice, Brn3 POU domain transcription factors play essential roles in the differentiation and survival of projection neurons within the retina, inner ear, dorsal root and trigeminal ganglia. During retinal ganglion cell differentiation, Brn3b is expressed first, followed by Brn3a and Brn3c. Targeted deletion of Brn3b, but not Brn3a or Brn3c, leads to a loss of most retinal ganglion cells before birth. However, as a few retinal ganglion cells are still present in Brn3b–/– mice, Brn3a and Brn3c may partially compensate for the loss of Brn3b. To examine the role of Brn3c in retinal ganglion cell development, we generated Brn3b/Brn3c double knockout mice and analyzed their retinas and optic chiasms. Retinal ganglion cell axons from double knockout mice were more severely affected than were those from Brn3b-deficient mice, indicating that Brn3c was required for retinal ganglion cell differentiation and could partially compensate for the loss of Brn3b. Moreover, Brn3c had functions in retinal ganglion cell differentiation separate from those of Brn3b. Ipsilateral and misrouted projections at the optic chiasm were overproduced in Brn3b–/– mice but missing were entirely in optic chiasms of Brn3b/Brn3c double knockout mice, suggesting that Brn3c controlled ipsilateral axon production. Forced expression of Brn3c in Brn3b–/– retinal explants restored neurite outgrowth, demonstrating that Brn3c could promote axon outgrowth in the absence of Brn3b. Our results reveal a complex genetic relationship between Brn3b and Brn3c in regulating the retinal ganglion cell axon outgrowth.

Methods of Retinal Ganglion Cell Differentiation From Pluripotent Stem Cells

2014 ◽  
Vol 3 (4) ◽  
pp. 2 ◽  
Author(s):  
Katherine P. Gill ◽  
Alex W. Hewitt ◽  
Kathryn C. Davidson ◽  
Alice Pébay ◽  
Raymond C. B. Wong
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Pluripotent Stem Cells ◽  
Retinal Ganglion
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