scholarly journals Anterior Duplication of the Sonic hedgehog Expression Pattern in the Pectoral Fin Buds of Zebrafish Treated with Retinoic Acid

1995 ◽  
Vol 170 (1) ◽  
pp. 243-247 ◽  
Author(s):  
Marie-Andrée Akimenko ◽  
Marc Ekker
Keyword(s):  
Retinoic Acid ◽  
Expression Pattern ◽  
Sonic Hedgehog ◽  
Pectoral Fin

Involvement of the sonic hedgehog, patched 1 and bmp2 genes in patterning of the zebrafish dermal fin rays

Development ◽  
1998 ◽  
Vol 125 (21) ◽  
pp. 4175-4184 ◽  
Author(s):  
L. Laforest ◽  
C.W. Brown ◽  
G. Poleo ◽  
J. Geraudie ◽  
M. Tada ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Sonic Hedgehog ◽  
Bone Matrix ◽  
Basal Layer ◽  
Pectoral Fin ◽  
Expression Domain ◽  
Fin Regeneration ◽  
Fin Rays ◽  
Dermal Bone ◽  
Fish Fins

The signaling molecule encoded by Sonic hedgehog (shh) participates in the patterning of several embryonic structures including limbs. During early fin development in zebrafish, a subset of cells in the posterior margin of pectoral fin buds express shh. We have shown that regulation of shh in pectoral fin buds is consistent with a role in mediating the activity of a structure analogous to the zone of polarizing activity (ZPA) (Akimenko and Ekker (1995) Dev. Biol. 170, 243–247). During growth of the bony rays of both paired and unpaired fins, and during fin regeneration, there does not seem to be a region equivalent to the ZPA and one would predict that shh would play a different role, if any, during these processes specific to fish fins. We have examined the expression of shh in the developing fins of 4-week old larvae and in regenerating fins of adults. A subset of cells in the basal layer of the epidermis in close proximity to the newly formed dermal bone structures of the fin rays, the lepidotrichia, express shh, and ptc1 which is thought to encode the receptor of the SHH signal. The expression domain of ptc1 is broader than that of shh and adjacent blastemal cells releasing the dermal bone matrix also express ptc1. Further observations indicate that the bmp2 gene, in addition to being expressed in the same cells of the basal layer of the epidermis as shh, is also expressed in a subset of the ptc1-expressing cells of the blastema. Amputations of caudal fins immediately after the first branching point of the lepidotrichia, and global administration of all-trans-retinoic acid, two procedures known to cause fusion of adjacent rays, result in a transient decrease in the expression of shh, ptc1 and bmp2. The effects of retinoic acid on shh expression occur within minutes after the onset of treatment suggesting direct regulation of shh by retinoic acid. These observations suggest a role for shh, ptc1 and bmp2 in patterning of the dermoskeleton of developing and regenerating teleost fins.

Transient establishment of anteroposterior polarity in the zebrafish pectoral fin bud in the absence of sonic hedgehog activity

Development ◽  
1999 ◽  
Vol 126 (21) ◽  
pp. 4817-4826 ◽  
Author(s):  
C.J. Neumann ◽  
H. Grandel ◽  
W. Gaffield ◽  
S. Schulte-Merker ◽  
C. Nusslein-Volhard
Keyword(s):  
Retinoic Acid ◽  
Sonic Hedgehog ◽  
Limb Development ◽  
Normal Development ◽  
Limb Bud ◽  
Pectoral Fin ◽  
Anteroposterior Patterning ◽  
Vertebrate Limb ◽  
Anteroposterior Polarity

Sonic hedgehog (Shh) is expressed in the posterior vertebrate limb bud mesenchyme and directs anteroposterior patterning and growth during limb development. Here we report an analysis of the pectoral fin phenotype of zebrafish sonic you mutants, which disrupt the shh gene. We show that Shh is required for the establishment of some aspects of anteroposterior polarity, while other aspects of anteroposterior polarity are established independently of Shh, and only later come to depend on Shh for their maintenance. We also demonstrate that Shh is required for the activation of posterior HoxD genes by retinoic acid. Finally, we show that Shh is required for normal development of the apical ectodermal fold, for growth of the fin bud, and for formation of the fin endoskeleton.

scholarly journals Identification and expression pattern of a second isoform of the newt alpha retinoic acid receptor

1992 ◽  
Vol 20 (21) ◽  
pp. 5851-5851 ◽  
Author(s):  
Clifton W. Ragsdale ◽  
Phillip B. Gates ◽  
Jeremy P. Brockes
Keyword(s):  
Retinoic Acid ◽  
Expression Pattern ◽  
Retinoic Acid Receptor ◽  
Acid Receptor

dackel acts in the ectoderm of the zebrafish pectoral fin bud to maintain AER signaling

Development ◽  
2000 ◽  
Vol 127 (19) ◽  
pp. 4169-4178 ◽  
Author(s):  
H. Grandel ◽  
B.W. Draper ◽  
S. Schulte-Merker
Keyword(s):  
Transcription Factor ◽  
Growth Factor ◽  
Sonic Hedgehog ◽  
Limb Development ◽  
Induction Phase ◽  
Fibroblast Growth ◽  
Pectoral Fin ◽  
Vertebrate Limb ◽  
Fibroblast Growth Factor 10 ◽  
Vertebrate Limb Development

Classical embryological studies have implied the existence of an apical ectodermal maintenance factor (AEMF) that sustains signaling from the apical ectodermal ridge (AER) during vertebrate limb development. Recent evidence suggests that AEMF activity is composed of different signals involving both a sonic hedgehog (Shh) signal and a fibroblast growth factor 10 (Fgf10) signal from the mesenchyme. In this study we show that the product of the dackel (dak) gene is one of the components that acts in the epidermis of the zebrafish pectoral fin bud to maintain signaling from the apical fold, which is homologous to the AER of tetrapods. dak acts synergistically with Shh to induce fgf4 and fgf8 expression but independently of Shh in promoting apical fold morphogenesis. The failure of dak mutant fin buds to progress from the initial fin induction phase to the autonomous outgrowth phase causes loss of both AER and Shh activity, and subsequently results in a proximodistal truncation of the fin, similar to the result obtained by ridge ablation experiments in the chicken. Further analysis of the dak mutant phenotype indicates that the activity of the transcription factor engrailed 1 (En1) in the ventral non-ridge ectoderm also depends on a maintenance signal probably provided by the ridge. This result uncovers a new interaction between the AER and the dorsoventral organizer in the zebrafish pectoral fin bud.

scholarly journals Axial (HNF3β) and retinoic acid receptors are regulators of the zebrafish sonic hedgehog promoter

1997 ◽  
Vol 16 (13) ◽  
pp. 3955-3964 ◽  
Author(s):  
Bei-En Chang ◽  
Patrick Blader ◽  
Nadine Fischer ◽  
Philip W. Ingham ◽  
Uwe Strähle
Keyword(s):  
Retinoic Acid ◽  
Sonic Hedgehog ◽  
Retinoic Acid Receptors
Sign in / Sign up

Export Citation Format

Share Document