Structure, Expression, and Hormonal Control of Genes from the Mosquito, Aedes aegypti, Which Encode Proteins Similar to the Vitelline Membrane Proteins of Drosophila melanogaster

1993 ◽  
Vol 155 (2) ◽  
pp. 558-568 ◽  
Author(s):  
Yonggu Lin ◽  
Martha T. Hamblin ◽  
Marten J. Edwards ◽  
Carolina Barillas-Mury ◽  
Michael R. Kanost ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Membrane Proteins ◽  
Aedes Aegypti ◽  
Hormonal Control ◽  
Vitelline Membrane

Comparative analysis of the sequence and structure of two Drosophila melanogaster genes encoding vitelline membrane proteins

Gene ◽  
1993 ◽  
Vol 136 (1-2) ◽  
pp. 121-127 ◽  
Author(s):  
L.J. Scherer ◽  
D.H. Harris ◽  
M.K. White ◽  
L.F. Steel ◽  
J. Jin ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Comparative Analysis ◽  
Membrane Proteins ◽  
Vitelline Membrane ◽  
Genes Encoding

Analysis of vitelline membrane proteins of fresh and stored eggs via HPLC

1998 ◽  
Vol 206 (5) ◽  
pp. 329-332 ◽  
Author(s):  
Annette Schäfer ◽  
Wiebke Drewes ◽  
F. Schwägele
Keyword(s):  
Membrane Proteins ◽  
Vitelline Membrane

scholarly journals Drosophila melanogaster Scramblases modulate synaptic transmission

2006 ◽  
Vol 173 (1) ◽  
pp. 69-82 ◽  
Author(s):  
Usha Acharya ◽  
Michael Beth Edwards ◽  
Ramon A. Jorquera ◽  
Hugo Silva ◽  
Kunio Nagashima ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Plasma Membrane ◽  
Membrane Proteins ◽  
Synaptic Transmission ◽  
Blood Cells ◽  
Neuromuscular Synapses ◽  
Neurotransmitter Secretion ◽  
Reserve Pool ◽  
Null Mutants

Scramblases are a family of single-pass plasma membrane proteins, identified by their purported ability to scramble phospholipids across the two layers of plasma membrane isolated from platelets and red blood cells. However, their true in vivo role has yet to be elucidated. We report the generation and isolation of null mutants of two Scramblases identified in Drosophila melanogaster. We demonstrate that flies lacking either or both of these Scramblases are not compromised in vivo in processes requiring scrambling of phospholipids. Instead, we show that D. melanogaster lacking both Scramblases have more vesicles and display enhanced recruitment from a reserve pool of vesicles and increased neurotransmitter secretion at the larval neuromuscular synapses. These defects are corrected by the introduction of a genomic copy of the Scramb 1 gene. The lack of phenotypes related to failure of scrambling and the neurophysiological analysis lead us to propose that Scramblases play a modulatory role in the process of neurotransmission.

Transgenic expression of the Aedes aegypti CYP9J28 confers pyrethroid resistance in Drosophila melanogaster

2012 ◽  
Vol 104 (2) ◽  
pp. 132-135 ◽  
Author(s):  
N. Pavlidi ◽  
M. Monastirioti ◽  
P. Daborn ◽  
I. Livadaras ◽  
T. Van Leeuwen ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Aedes Aegypti ◽  
Pyrethroid Resistance ◽  
Transgenic Expression

scholarly journals A wMel Wolbachia variant in Aedes aegypti from field-collected Drosophila melanogaster with increased phenotypic stability under heat stress

2022 ◽  
Author(s):  
Xinyue Gu ◽  
Perran A Ross ◽  
Julio Rodriguez-Andres ◽  
Katie L. Robinson ◽  
Qiong Yang ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Stress ◽  
Aedes Aegypti ◽  
Cytoplasmic Incompatibility ◽  
Vector Competence ◽  
Temperature Tolerance ◽  
Seasonal Temperature ◽  
Maternal Transmission ◽  
Tropical Regions ◽  
Host Fitness

Mosquito-borne diseases such as dengue, Zika and chikungunya remain a major cause of morbidity and mortality across tropical regions. Population replacement strategies involving the wMel strain of Wolbachia are being used widely to control mosquito-borne diseases transmitted by Aedes aegypti. However, these strategies may be influenced by environmental temperature because wMel is vulnerable to heat stress. wMel infections in their native host Drosophila melanogaster are genetically diverse, but few transinfections of wMel variants have been generated in Ae. aegypti mosquitoes. Here we successfully transferred a wMel variant (termed wMelM) originating from a field-collected D. melanogaster population from Victoria, Australia into Ae. aegypti. The new wMelM variant (clade I) is genetically distinct from the original wMel transinfection (clade III) generated over ten years ago, and there are no genomic differences between wMelM in its original and transinfected host. We compared wMelM with wMel in its effects on host fitness, temperature tolerance, Wolbachia density, vector competence, cytoplasmic incompatibility and maternal transmission under heat stress in a controlled background. wMelM showed a higher heat tolerance than wMel, with stronger cytoplasmic incompatibility and maternal transmission when eggs were exposed to heat stress, likely due to higher overall densities within the mosquito. Both wMel variants had minimal host fitness costs, complete cytoplasmic incompatibility and maternal transmission, and dengue virus blocking under standard laboratory conditions. Our results highlight phenotypic differences between closely related Wolbachia variants. wMelM shows potential as an alternative strain to wMel in dengue control programs in areas with strong seasonal temperature fluctuations.

scholarly journals Vitelline membrane proteins promote left-sided nodal expression after neurula rotation in the ascidian, Halocynthia roretzi

2019 ◽  
Vol 449 (1) ◽  
pp. 52-61 ◽  
Author(s):  
Yuka Tanaka ◽  
Shiori Yamada ◽  
Samantha L. Connop ◽  
Noritaka Hashii ◽  
Hitoshi Sawada ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Vitelline Membrane ◽  
Halocynthia Roretzi
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