ANTIGENIC AND ULTRASTRUCTURAL MARKERS ASSOCIATED WITH UROTHELIAL CYTODIFFERENTIATION IN PRIMARY EXPLANT OUTGROWTHS OF MOUSE BLADDER

2002 ◽  
Vol 26 (1) ◽  
pp. 63-74 ◽  
Author(s):  
M Kreft
Keyword(s):  
Primary Explant

The Culture of Small Aggregates of Amphibian Embryonic Cells in vitro

Development ◽  
1963 ◽  
Vol 11 (1) ◽  
pp. 135-154
Author(s):  
K. W. Jones ◽  
T. R. Elsdale
Keyword(s):  
Pigment Cells ◽  
Two Dimensional ◽  
Embryonic Cells ◽  
Common Procedure ◽  
Large Numbers ◽  
Organ Type ◽  
Typical Cell ◽  
Primary Explant ◽  
Migration Of Cells

A Common procedure in amphibian embryology has been to remove portions from embryos and culture these under conditions in which the large numbers of cells retain a close-knit association, favourable to the differentiation of primitive organs in the explant. It has not, in general, been the aim to employ the primary explant as a source of a two-dimensional outgrowth of cells on the substrate, as in typical cell culture procedures. Because of their inherent migratory tendencies, however, outgrowths of pigment cells are readily obtained from explants of the amphibian neural crest, and these have stimulated the interest of a number of investigators (see Wilde, 1961). Holtfreter (1938, 1946) and Finnegan (1953) have also observed the migration of cells from explants of Urodele embryos. Several investigators have employed cell cultures as opposed to organ type cultures in induction studies, Niu & Twitty (1953), Niu (1958), Barth & Barth (1959) and Becker, Tiedemann & Tiedemann (1959).

scholarly journals The Detroit Strains of Human Epithelial-like Cells from Nonleukemic Peripheral Blood

Blood ◽  
1958 ◽  
Vol 13 (12) ◽  
pp. 1149-1167 ◽  
Author(s):  
LAWRENCE BERMAN ◽  
CYRIL S. STULBERG
Keyword(s):  
Blood Cell ◽  
Human Blood ◽  
Peripheral Blood ◽  
Blood Cultures ◽  
L Cells ◽  
Primary Explant ◽  
Cell Strains

Abstract 1. The development of 6 strains of Ep-L cells from human blood was reported. In each instance the Ep-L cells appeared in cultures which had some connection with the administration of estrogenic materials, either to the donor of the blood or to the primary explant of blood in vitro. The possible role of estrogens was discussed, but no final conclusions were reached. 2. The development of stable human Ep-L cells did not require the participation of cells from leukemic blood. 3. Morphologic, karyotypic, and mitotic data on the Detroit blood cell strains were presented. 4. The morphogenesis of the Ep-L cells was described. The most likely source of the Ep-L cells in blood cultures appeared to be lymphocytes, but the role of monocytes could not be ruled out. 5. The significance of our findings with respect to transplantation studies was indicated.

scholarly journals STUDIES ON THE PROPAGATION IN VITRO OF POLIOMYELITIS VIRUSES

1952 ◽  
Vol 96 (4) ◽  
pp. 389-400 ◽  
Author(s):  
William F. Scherer ◽  
Jerome T. Syverton
Keyword(s):  
Virus Type ◽  
Testicular Tissue ◽  
Nerve Cells ◽  
Specific Antibodies ◽  
Poliomyelitis Virus ◽  
Explant Cultures ◽  
Pure Cultures ◽  
Primary Explant

Cells like fibroblasts, having no resemblance whatever to nerve cells were obtained in morphologically pure cultures from monkey testicular tissue and were found to support the growth in vitro of poliomyelitis virus, Type 2, Yale-SK strain. Moreover, these cells were destroyed as a result of the multiplication of this virus within them. Similarly, "fibroblasts" propagated in primary explant cultures of testicle were destroyed by poliomyelitis viruses, Types 1 and 2. Type specific antibodies neutralized the pathogenic effect of poliomyelitis virus on monkey testicular fibroblasts.

scholarly journals Chemical Compositions, Somatic Embryogenesis, and Somaclonal Variation in Cumin

2017 ◽  
Vol 2017 ◽  
pp. 1-15 ◽  
Author(s):  
Moslem Bahmankar ◽  
Seyed Mohammad Mahdi Mortazavian ◽  
Masoud Tohidfar ◽  
Seyed Ahmad Sadat Noori ◽  
Ali Izadi Darbandi ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Ascorbate Peroxidase ◽  
Developmental Stages ◽  
Chemical Compositions ◽  
Breeding Programs ◽  
Somaclonal Variations ◽  
Regenerated Plants ◽  
Mother Plants ◽  
Primary Explant ◽  
The Relationship

This is the first report evaluating the relationship between the chemical compositions of cumin seeds (based on the analysis of the content of catalase, ascorbate peroxidase, proline, protein, terpenic compounds, alcohol/phenols, aldehydes, and epoxides) and the induction efficiency of somatic embryogenesis in two Iranian superior cumin landraces (Golestan and North Khorasan). Cotyledons isolated from Golestan landrace seeds cultivated on MS medium supplemented with 0.1 mg/L kinetin proved to be the best primary explant for the induction of somatic embryogenesis as well as the regeneration of the whole plantlet. Results indicated that different developmental stages of somatic embryos were simultaneously observed on a callus with embryogenic potential. The high content of catalase, ascorbate peroxidase, proline, and terpenic hydrocarbons and low content of alcoholic and phenolic compositions had a stimulatory effect on somatic embryogenesis. Band patterns of RAPD markers in regenerated plants were different from those of the mother plants. This may be related to somaclonal variations or pollination system of cumin. Generally, measurement of chemical compositions can be used as a marker for evaluating the occurrence of somatic embryogenesis in cumin. Also, somaclonal variations of regenerated plants can be applied by the plant breeders in breeding programs.

A sequential cell kinetic study of meningioma cells in primary explant culture using bromodeoxyuridine

1993 ◽  
Vol 16 (2) ◽  
pp. 117-123
Author(s):  
Kusum Kharbanda ◽  
Asis K. Karak ◽  
Chitra Sarkar ◽  
Amit K. Dinda ◽  
Meera Mathur ◽  
...  
Keyword(s):  
Kinetic Study ◽  
Explant Culture ◽  
Cell Kinetic ◽  
Primary Explant
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