3,4-Dihydro-3,4,6-trimethyl-2H,8H-pyrano-[3,2g]-1,3-benzoxazin-2,8-dione, a Potential Fluorogenic Reporter Group Reagent for Esterases—Synthesis and Interaction with Chymotrypsin

2000 ◽  
Vol 28 (5) ◽  
pp. 273-282
Author(s):  
Trevor M. Kitson ◽  
Graham H. Freeman
Keyword(s):  
Reporter Group

The 2,2,2-trifluoroethyl ligand: a structural "reporter" group for its carbanion character in organometallic compounds

1985 ◽  
Vol 4 (4) ◽  
pp. 630-636 ◽  
Author(s):  
Nevenka. Bresciani-Pahor ◽  
Mario. Calligaris ◽  
Lucio. Randaccio ◽  
Luigi G. Marzilli ◽  
Michael F. Summers ◽  
...  
Keyword(s):  
Organometallic Compounds ◽  
Reporter Group

Monitoring Cu2+-Binding to a DNA-Clip-phen Conjugate and Metal-centered Redox Processes by a Fluorescent Reporter Group

2013 ◽  
Vol 639 (8-9) ◽  
pp. 1636-1639 ◽  
Author(s):  
Arndt Sprödefeld ◽  
Alexander Kiel ◽  
Dirk-Peter Herten ◽  
Roland Krämer
Keyword(s):  
Redox Processes ◽  
Fluorescent Reporter ◽  
Reporter Group

Self-immolative base-mediated conjugate release from triazolylmethylcarbamates

2015 ◽  
Vol 13 (32) ◽  
pp. 8703-8707 ◽  
Author(s):  
Christopher A. Blencowe ◽  
David W. Thornthwaite ◽  
Wayne Hayes ◽  
Andrew T. Russell
Keyword(s):  
Aromatic Amine ◽  
Reporter Group

A range of carbamate functionalized 1,4-disubstituted triazoles featuring a model aromatic amine reporter group (R) have been prepared via copper(i) catalysed azide–alkyne cycloaddition and revealed self-immolative characteristics under basic conditions.

scholarly journals Design and properties of a fluorescent indicator of intracellular free Na+ concentration

1988 ◽  
Vol 250 (1) ◽  
pp. 227-232 ◽  
Author(s):  
G A Smith ◽  
T R Hesketh ◽  
J C Metcalfe
Keyword(s):  
Aqueous Solubility ◽  
Ratio Method ◽  
Isosbestic Point ◽  
Buffer Solution ◽  
Acetoxymethyl Ester ◽  
Apparent Dissociation Constant ◽  
Fluorescent Indicator ◽  
Carboxylate Groups ◽  
Reporter Group ◽  
Benzenoid Ring

We have recently described a cryptand structure, FCryp-1, with appropriate properties for an indicator of intracellular free Na+ concentration using the 19F-n.m.r. chemical shift of the incorporated 5FBAPTA [1,2-bis-(2-amino-5-fluorophenoxy)ethane-NNN‘N’-tetra-acetic acid] reporter group to measure the free cytosolic Na+ concentration [(Na+]i) [Smith, Morris, Hesketh and Metcalfe (1986) Biochim. Biophys. Acta 889, 82-83]. FCryp-1 carries four carboxylate groups to confer aqueous solubility and the indicator is membrane-permeant when the carboxyls are esterified with acetoxymethyl ester groups. Here we describe the synthesis of FCryp-2 to provide a fluorescent indicator of [Na+]i. FCryp-2 retains the parent tribenzo (2:2:1) cryptand structure of FCryp-1, in which the benzenoid ring at C-21 in FCryp-1 is replaced by an indole derivative which acts as the fluorophor in FCryp-2. With excitation at 340 nm, FCryp-2 gives an emission maximum at 460 nm in the absence of Na+ which shifts to 395 nm when FCryp-2 is saturated with Na+, with an isosbestic point at 455 nm. The apparent dissociation constant of FCryp-2 in a buffer solution of 100 mM-KCl/20 mM-KH2PO4/K2HPO4, pH 7.0, at 37 degrees C is 6.0 mM and the free Na+ concentration can be measured either from the calibrated fluorescence intensity at 395 nm, which increases 25-fold when Na+ is bound to FCryp-2, or from the ratio of fluorescence intensities at 395 nm and 455 nm. The measurement of free [Na+] by either method is unaffected by K+, Ca2+ or Mg2+ in the normal intracellular concentration ranges. Free [Na+] measurements by the ratio method are unaffected by pH from 6.6 to 7.6.

scholarly journals Ca2+-dependent and phospholipid-independent binding of annexin 2 and annexin 5

2002 ◽  
Vol 367 (3) ◽  
pp. 895-900 ◽  
Author(s):  
Nicole D. BROOKS ◽  
Jean E. GRUNDY ◽  
Nadine LAVIGNE ◽  
Mélanie C. DERRY ◽  
Christina M. RESTALL ◽  
...  
Keyword(s):  
Binding Sites ◽  
Dependent Manner ◽  
Homologous Proteins ◽  
Anionic Phospholipid ◽  
Apparent Dissociation Constant ◽  
Excess Amount ◽  
Annexin 2 ◽  
Covalently Linked ◽  
Reporter Group ◽  
Dissociation Constant Kd

Annexins are a family of homologous proteins that associate with anionic phospholipid (aPL) in the presence of Ca2+. Evidence that the function of one annexin type may be regulated by another was recently reported in studies investigating cytomegalovirus—aPL interactions, where the fusogenic function of annexin 2 (A2) was attenuated by annexin 5 (A5). This observation suggested that A2 may bind directly to A5. In the present study, we demonstrated this interaction. The A2—A5 complex was first detected utilizing (covalently linked) fluorescein-labelled A5 (F-A5) as a reporter group. The interaction required concentrations of Ca2+ in the millimolar range, had an apparent dissociation constant [Kd(app)] of 1nM at 2mM Ca2+ and was independent of aPL. A2 bound comparably with F-A5 pre-equilibrated with an amount of aPL that could bind just the F-A5 or to an excess amount of aPL providing sufficient binding sites for all of F-A5 and A2. A2—A5 complex formation was corroborated in an experiment, where [125I]A2 associated in a Ca2+-dependent manner with A5 coated on to polystyrene. Surface plasmon resonance was used as a third independent method to demonstrate the binding of A2 and A5 and, furthermore, supported the conclusion that the monomeric and tetrameric forms of A2 bind equivalently to A5. Together these results demonstrate an A2—A5 interaction and provide an explanation as to how A5 inhibits the previously reported A2-dependent enhancement of virus—aPL fusion.

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