Occurrence of Antibiotic Resistance in the Biological Control of Potato Scab Disease

1999 ◽  
Vol 16 (2) ◽  
pp. 199-208 ◽  
Author(s):  
Eric C Neeno-Eckwall ◽  
Janet L Schottel
Keyword(s):  
Biological Control ◽  
Antibiotic Resistance ◽  
Potato Scab ◽  
Potato Scab Disease

scholarly journals Necrosis Gene-Based Monitoring and Control of Potato Scab Disease

2007 ◽  
Vol 22 (2) ◽  
pp. 123-127 ◽  
Author(s):  
Osamu Koyama ◽  
Akira Manome ◽  
Mayu Okubo ◽  
Toyokazu Yokomaku ◽  
Hideo Tanaka
Keyword(s):  
Potato Scab ◽  
Monitoring And Control ◽  
Potato Scab Disease ◽  
And Control

Biological control of potato scab and antibiosis by antagonistic Streptomyces sp. WoRs-501

2015 ◽  
Vol 81 (6) ◽  
pp. 439-448 ◽  
Author(s):  
Yuki O. Kobayashi ◽  
Akira Kobayashi ◽  
Masayuki Maeda ◽  
Nobutaka Someya ◽  
Shigehito Takenaka
Keyword(s):  
Biological Control ◽  
Potato Scab ◽  
Streptomyces Sp ◽  
Antagonistic Streptomyces

Competition and antibiosis in the biological control of potato scab

2001 ◽  
Vol 47 (4) ◽  
pp. 332-340 ◽  
Author(s):  
Eric C Neeno-Eckwall ◽  
Linda L Kinkel ◽  
Janet L Schottel
Keyword(s):  
Biological Control ◽  
Population Density ◽  
Potato Scab ◽  
Antibiotic Activity ◽  
Streptomyces Scabies ◽  
Population Densities ◽  
Streptomyces Diastatochromogenes ◽  
Greenhouse Assay ◽  
Spontaneous Mutants

Nonpathogenic, antibiotic-producing streptomycetes have been shown to reduce potato scab when added to disease-conducive soil. Spontaneous mutants of the pathogenic Streptomyces scabies RB4 that are resistant to at least one antibiotic activity produced by the nonpathogenic suppressive isolates Streptomyces diastatochromogenes strain PonSSII and S. scabies PonR have been isolated. To determine the importance of antibiosis in this biocontrol system, these mutants were investigated for their ability to cause disease in the presence of the two pathogen antagonists in a greenhouse assay. Disease caused by one of the mutant strains was reduced in the presence of both suppressive isolates, whereas disease caused by the other five mutants was not significantly reduced by either suppressive strain. In addition, a nonpathogenic mutant of S. scabies RB4 was isolated, which produced no detectable in vitro antibiotic activity and reduced disease caused by its pathogenic parent strain when the pathogen and mutant were coinoculated into soil. Population densities of the pathogen were consistently lower than those of the suppressive strains when individual strains were inoculated into soil. When a pathogen was coinoculated with a suppressive strain, the total streptomycete population density in the pot was always less than that observed when the suppressive isolate was inoculated alone. When the pathogens were inoculated individually into soil, a positive correlation was seen between population density and disease severity. In coinoculation experiments with pathogen and suppressive strains, higher total streptomycete population densities were correlated with lower amounts of disease.Key words: Streptomyces scabies, biological control, antibiotic resistance, potato scab disease.

Differentiation of Streptomyces spp. which cause potato scab disease on the basis of partial rpoB gene sequences

2007 ◽  
Vol 30 (5) ◽  
pp. 401-407 ◽  
Author(s):  
Ho-Suk Mun ◽  
Eun-Ju Oh ◽  
Hyun-Ju Kim ◽  
Keun-Hwa Lee ◽  
Young-Hwan Koh ◽  
...  
Keyword(s):  
Potato Scab ◽  
Rpob Gene ◽  
Gene Sequences ◽  
Streptomyces Spp ◽  
Potato Scab Disease

scholarly journals Retraction: Study on Molecular Qualitative Detection Technology of Potato Scab Disease (IOP Conf. Ser.: Earth Environ. Sci. 769 022010)

2021 ◽  
Vol 769 (2) ◽  
pp. 022076
Keyword(s):  
Fragment Size ◽  
Potato Scab ◽  
Detection Technology ◽  
Qualitative Detection ◽  
Potato Scab Disease ◽  
Different Strains ◽  
Repeated Experiment

This article has been retracted by the authors following correspondence with IOP Publishing in which the authors claim the work is unreliable. The author's explanation follows: "The material of the paper is bacteria, it is not be visible to the naked eyes, the material was contaminated by the drugs that out of date and got polluted, and it caused the serious mistake in the figure 2. The result of different strains of DNA by the primer in 3.3 was not match of our material. The figure 2 was the amplification results of different strains of DNA , and the fragment size of different strains were same in the result, but the result should not be same , so the result was wrong. When we continue to the study, we got new result about the figure 2. There was the amplification results of different strains of DNA by primer TXT in repeated experiment , and the result in the published article was wrong, the material was contaminated. We are afraid that other results were also incorrect in this paper." IOP Publishing cannot verify this information as accurate, however in the interest of transparency and reproducibility, IOP Publishing agrees to retract this article. This notice will be updated if more information comes to light. Retraction published: 21 December 2021

Influence of disease-suppressive strains of Streptomyces on the native Streptomyces community in soil as determined by the analysis of cellular fatty acids

1996 ◽  
Vol 42 (1) ◽  
pp. 27-37 ◽  
Author(s):  
John H. Bowers ◽  
Linda L. Kinkel ◽  
Roger K. Jones
Keyword(s):  
Biological Control ◽  
Fatty Acid ◽  
Potato Scab ◽  
Cellular Fatty Acid ◽  
Fatty Acid Analysis ◽  
Community Diversity ◽  
Group Method ◽  
Fatty Acid Profiles ◽  
Field Plots ◽  
Related Population

Analysis of cellular fatty acid profiles was used to distinguish among introduced pathogen- suppressive strains and indigenous strains of Streptomyces spp. isolated from soil of field plots established to test the efficacy of Streptomyces strains PonSSII and PonR in the biological control of potato scab. Reference libraries of fatty acid profiles were developed for a collection of known pathogenic strains and the introduced suppressive strains. Population densities of pathogen-related, suppressive, and saprophytic Streptomyces strains were determined from the relationship of field isolates to mean library profiles using cluster analysis and the unweighted pair-group method using arithmetic averages. Community diversity was similarly determined. Streptomyces strains PonSSII and PonR were distinguished from each other and from the pathogen group (which clustered together) based on fatty acid profiles. The introduced, suppressive strains successfully colonized the soil and represented 2–19% of the isolates sampled over 2 years. The introduction of the suppressive strains inhibited the population of strains related to the pathogen library at each sample date; the pathogen population was substantially lower in soil from treatments where the suppressive strains were introduced compared with the nonamended control. At harvest, the pathogen-related population was suppressed 85–93 and 36–44% in 1991 and 1992, respectively, in treatments with the suppressive strains compared with the nonamended control. Diversity of the community was not affected by the introduced strains, and diversity and equitability indices were similar among treatments at any sample time. The inhibition of the pathogen-related population was correlated with a reduction of scab symptoms observed in the field plots into which the suppressive strains were introduced. Implications of a fundamental shift in the pathogen-related population in response to the introduction of the suppressive strains for long-term biological control of potato scab are encouraging.Key words: Streptomyces, fatty acid analysis, biological control, community ecology.

scholarly journals First Report Regarding Potato Scab Caused by Streptomyces acidiscabies in Uruguay

Plant Disease ◽  
2012 ◽  
Vol 96 (7) ◽  
pp. 1064-1064 ◽  
Author(s):  
M. I. Lapaz ◽  
E. Verdier ◽  
M. J. Pianzzola
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Potato Scab ◽  
Rrna Gene ◽  
Genus Streptomyces ◽  
Pathogenicity Genes ◽  
First Report ◽  
Streptomyces Acidiscabies ◽  
Potato Scab Disease ◽  
The 16S Rrna Gene

Potato scab disease is caused by gram-positive filamentous bacteria in the genus Streptomyces. A great variety of species cause this disease, but Streptomyces scabies is the most ancient of these pathogens and can be found in a worldwide distribution, whereas S. turgidiscabies and S. acidiscabies are newly emerged pathogens (2). During the autumn of 2010, potato (Solanum tuberosum) crops had large economic losses by common scab, corresponding to 29% of the total potato-cultivation area (according to our survey), which was unusual in Uruguay. Specifically, the disease was very aggressive and the tubers showed particularly deep scab lesions. We isolated the Streptomyces species present in these particular scab lesions of tubers collected in July 2010 from one of the three potato cultivation areas (San José). A total of 19 Streptomyces spp. strains were isolated and identified using classical and molecular techniques. Morphological characteristics of colonies and microscopic structure of the mycelium were observed (1). Molecular characterization by conventional PCR was carried out using primers directed to specific regions of the 16S rRNA gene for the genus Streptomyces, Aci1: (5′-TCACTCCTGCCTGCATGGGCG-3′) and Aci2: (5′-CGACAGCTCCCTCCCACAAG-3′). Also, regions of two pathogenicity genes, namely txtAB and nec1, were amplified and confirmed by sequencing (2). Additionally, melanin production and pathogenicity of the isolates was determined by inoculation of potato discs (1). Six of the 19 strains succeeded in PCR amplification with primers specific to Streptomyces acidiscabies, which has white, aerial hypha and flexuous spore chains. These strains did not produce melanin on tyrosine agar media. The amplified fragments for 16S rRNA and pathogenicity genes from one representative strain 61 were sequenced. BLASTn analysis of the 16S rRNA gene sequence obtained of the strain 61 (Accession No. JN206667) showed the highest similarity (100%) with S. acidiscabies type strain 84-01-182 (GenBank Accession No. FJ007427.1). Pathogenicity of the isolate was tested on tuber slices. The isolate was grown on YME for 5 to 7 days at 28°C and agar plugs from the sporulating colonies were inverted onto excised tuber tissue. Disks were incubated at 28°C in the dark and the presence of necrosis was evaluated after 5 days (1). All tuber slice assays were repeated three times. The noninoculated control tuber slices did not show any necrosis, while those inoculated with the strain did. To our knowledge, this is the first report of S. acidiscabies causing potato scab disease in Uruguay. References: (1) D. H. Park et al. Plant Dis. 87:1290, 2003. (2) L. A. Wanner. Phytopathology 96:1361, 2006.

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