NF-Y Involvement in the Polyunsaturated Fat Inhibition of Fatty Acid Synthase Gene Transcription

2002 ◽  
Vol 290 (4) ◽  
pp. 1295-1299 ◽  
Author(s):  
Margarita Teran-Garcia ◽  
Caterina Rufo ◽  
Manabu T. Nakamura ◽  
Timothy F. Osborne ◽  
Steven D. Clarke
Keyword(s):  
Fatty Acid ◽  
Gene Transcription ◽  
Fatty Acid Synthase ◽  
Polyunsaturated Fat ◽  
Fatty Acid Synthase Gene

Transcriptional regulation of the adipocyte fatty acid synthase gene by agouti: interaction with insulin

2000 ◽  
Vol 3 (3) ◽  
pp. 157-162 ◽  
Author(s):  
KATE J. CLAYCOMBE ◽  
YANXIN WANG ◽  
BRYNN H. JONES ◽  
SUYEON KIM ◽  
WILLIAM O. WILKISON ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Fatty Acid ◽  
Gene Transcription ◽  
Fatty Acid Synthase ◽  
Fusion Gene ◽  
Ectopic Expression ◽  
Fold Increase ◽  
Transcriptional Level ◽  
Additive Effects ◽  
Fatty Acid Synthase Gene

Claycombe, Kate J., Yanxin Wang, Brynn H. Jones, Suyeon Kim, William O. Wilkison, Michael B. Zemel, Joseph Chun, and Naima Moustaid-Moussa. Transcriptional regulation of the adipocyte fatty acid synthase gene by agouti: interaction with insulin. Physiol Genomics 3: 157–162, 2000.—Mice carrying dominant mutations at the agouti locus exhibit ectopic expression of agouti gene transcripts, obesity, and type II diabetes through unknown mechanisms. To gain insight into the role of agouti protein in modulating adiposity, we investigated regulation of a key lipogenic gene, fatty acid synthase (FAS) by agouti alone and in combination with insulin. Both agouti and insulin increase FAS activity in 3T3-L1 and in human adipocytes. Agouti and insulin independently and additively increase FAS activity in 3T3-L1 adipocytes. We further investigated the mechanism responsible for the agouti-induced FAS expression in these cells and demonstrated that both insulin (3-fold increase) and agouti (2-fold) increased FAS gene expression at the transcriptional level. Furthermore, insulin and agouti together exerted additive effects (5-fold increase) on FAS gene transcription. Transfection assays of FAS promoter-luciferase fusion gene constructs into 3T3-L1 adipocytes indicated that the agouti response element(s) is (are) located in the −435 to −415 region (−435/−415) of the FAS promoter. Nuclear proteins binding to this novel sequence are adipocyte specific. Thus the agouti response sequences mapped to a region upstream of the insulin-responsive element (which we previously reported to be located at −67/−52), consistent with additive effects of these two factors on FAS gene transcription.

scholarly journals Involvement of a Unique Carbohydrate-responsive Factor in the Glucose Regulation of Rat Liver Fatty-acid Synthase Gene Transcription

2001 ◽  
Vol 276 (24) ◽  
pp. 21969-21975 ◽  
Author(s):  
Caterina Rufo ◽  
Margarita Teran-Garcia ◽  
Manabu T. Nakamura ◽  
Seung-Hoi Koo ◽  
Howard C. Towle ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Gene Transcription ◽  
Rat Liver ◽  
Fatty Acid Synthase ◽  
Glucose Regulation ◽  
Liver Fatty Acid ◽  
Fatty Acid Synthase Gene
Sign in / Sign up

Export Citation Format

Share Document