Capacitation Induces Tyrosine Phosphorylation of Proteins in the Boar Sperm Plasma Membrane

1999 ◽  
Vol 262 (3) ◽  
pp. 787-792 ◽  
Author(s):  
Frits M. Flesch ◽  
Ben Colenbrander ◽  
Lambert M.G. van Golde ◽  
Barend M. Gadella
Keyword(s):  
Plasma Membrane ◽  
Tyrosine Phosphorylation ◽  
Phosphorylation Of Proteins ◽  
Boar Sperm ◽  
Sperm Plasma Membrane

scholarly journals Electrophoretic Map of Boar Sperm Plasma Membrane Polypeptides and Localization and Fractionation of Specific Polypeptide Subclasses

1983 ◽  
Vol 28 (2) ◽  
pp. 393-413 ◽  
Author(s):  
Lonnie D. Russell ◽  
R. N. Peterson ◽  
Terri A. Russell ◽  
Wally Hunt
Keyword(s):  
Plasma Membrane ◽  
Boar Sperm ◽  
Sperm Plasma Membrane

Effect of CB1 Receptors on Boar Sperm Plasma Membrane

2007 ◽  
Vol 31 (S1) ◽  
pp. 189-191
Author(s):  
M. G. Pistilli ◽  
N. Bernabò ◽  
A. Gloria ◽  
M. Mattioli ◽  
B. Barboni
Keyword(s):  
Plasma Membrane ◽  
Cb1 Receptors ◽  
Boar Sperm ◽  
Sperm Plasma Membrane

The interaction of living boar sperm and sperm plasma membrane vesicles with the porcine zona pellucida

1981 ◽  
Vol 84 (1) ◽  
pp. 144-156 ◽  
Author(s):  
R.N. Peterson ◽  
L.D. Russell ◽  
D. Bundman ◽  
M. Conway ◽  
M. Freund
Keyword(s):  
Plasma Membrane ◽  
Zona Pellucida ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles ◽  
Boar Sperm ◽  
Sperm Plasma Membrane

The Effect of Melatonin On Boar Sperm Plasma Membrane Fluidity and Cryosurvival

Cryobiology ◽  
2021 ◽  
Vol 103 ◽  
pp. 190
Author(s):  
Norma A. Ramirez-Campos ◽  
Alicia Alcantar-Rodriguez ◽  
Oscar Gutierrez-Perez ◽  
Alfredo Medrano
Keyword(s):  
Plasma Membrane ◽  
Membrane Fluidity ◽  
Plasma Membrane Fluidity ◽  
Boar Sperm ◽  
Sperm Plasma Membrane

scholarly journals Increase in the concentration of major boar sperm surface proteins during maturation in the epididymis

1986 ◽  
Vol 82 (1) ◽  
pp. 295-308
Author(s):  
N.K. Saxena ◽  
N. Saxena ◽  
W. Hunt ◽  
R.N. Peterson ◽  
L. Henry ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Polyacrylamide Gel Electrophoresis ◽  
Surface Protein ◽  
Surface Proteins ◽  
Secretory Cells ◽  
Boar Sperm ◽  
Sperm Plasma Membrane ◽  
Antigen Localization ◽  
Polyclonal Antisera ◽  
Narrow Area

High-resolution two-dimensional polyacrylamide gel electrophoresis analyses have indicated that several major boar sperm plasma membrane polypeptides (PMPs) increased in concentration during maturation in the epididymis. To investigate this further, monoclonal antibodies (MAbs) to two of these PMPs/glycoproteins referenced as 4.85 and 5.0 and polyclonal antisera (PCA) raised against PMP 5.0 were used to quantify these changes. ELISA assays for both PMPs, using solubilized plasma membrane (PM) and intact PM as antigens, indicated that both PMPs were present in greater concentration in cauda PM than in caput PM. ELISA assay using PCA against 5.0 and whole sperm from the cauda and caput epididymis also showed an increase in the concentration of this protein during transit through the epididymis. Indirect FITC fluorescence microscopy showed that MAbs reacted specifically with the luminal aspect of secretory cells of the corpus epididymis and, with greater intensity, with secretory cells of the cauda segment. When MAb tags were used the fluorescence technique indicated that PMP 5.0 was restricted to a narrow area of PM overlying the principal segment of the head of caput sperm but fluorescence extended further into the principal segment and into the flagellum of cauda PM. Since MAb binding sites appear to be partially masked, PCA were also used to localize antigenic sites. PCA to PMP 5.0 showed antigen localization in both head and flagellar PM of cauda and caput sperm. The fluorescence of cauda sperm PM, however, was significantly more intense than that of caput sperm PM. MAb to PMP 4.85 also bound to antigen more extensively on cauda sperm. The sum of these analyses suggests that major sperm PMPs are secreted by the epididymis and absorbed by sperm. The magnitude of surface protein changes occurring during epididymal transit in boar sperm appears to be greater than heretofore recognized.

Further characterization of boar sperm plasma membrane proteins with affinity for the porcine zona pellucida

1985 ◽  
Vol 12 (1) ◽  
pp. 91-100 ◽  
Author(s):  
R. N. Peterson ◽  
L. Henry ◽  
W. Hunt ◽  
N. Saxena ◽  
L. D. Russell
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Zona Pellucida ◽  
Plasma Membrane Proteins ◽  
Boar Sperm ◽  
Sperm Plasma Membrane
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