Release of Soluble ICAM-1 from Human Lung Fibroblasts, Aortic Smooth Muscle Cells, Dermal Microvascular Endothelial Cells, Bronchial Epithelial Cells, and Keratinocytes

1999 ◽  
Vol 260 (3) ◽  
pp. 734-739 ◽  
Author(s):  
Kam H. Leung
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Epithelial Cells ◽  
Smooth Muscle Cells ◽  
Human Lung ◽  
Lung Fibroblasts ◽  
Microvascular Endothelial Cells ◽  
Bronchial Epithelial ◽  
Human Lung Fibroblasts ◽  
Aortic Smooth Muscle

scholarly journals Sulphation by cultured cells. Cysteine, cysteinesulphinic acid and sulphite as sources for proteoglycan sulphate

1988 ◽  
Vol 252 (1) ◽  
pp. 305-308 ◽  
Author(s):  
D E Humphries ◽  
C K Silbert ◽  
J E Silbert
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Cultured Cells ◽  
Chondroitin Sulphate ◽  
Muscle Cells ◽  
Lung Fibroblasts ◽  
Human Embryonic Lung ◽  
Aortic Endothelial Cells ◽  
Aortic Smooth Muscle

Bovine aortic smooth-muscle cells, bovine aortic endothelial cells, and IMR-90 human embryonic lung fibroblasts were tested to determine their ability to use cysteine or cysteine metabolites as a source of sulphate (SO4). Cells were incubated in SO4-depleted medium containing [3H]glucosamine plus 0.2 mM-cystine, 0.3 mM-cysteinesulphinic acid or 0.3 mM-sulphite (SO3). The [3H]chondroitin sulphate produced by the different cells was found to vary considerably in degree of sulphation under these conditions. One line of smooth-muscle cells utilized cysteine effectively as a SO4 source and thus produced chondroitin sulphate which was highly sulphated. IMR-90 fibroblasts produced partly sulphated chondroitin sulphate under these conditions, while another smooth-muscle cell line could not utilize cysteine, but could utilize cysteinesulphinic acid as a partial SO4 source. In contrast with the above cells, endothelial cells could not use cysteine or cysteinesulphinic acid as a source of SO4 and produced chondroitin with almost no SO4. All of the cells were able to utilize SO3. Incubation of the cells in the SO4-depleted medium containing [35S]cysteine confirmed that only the first line of smooth-muscle cells could convert significant amounts of [35S]cysteine to 35SO4. Furthermore, the addition of 0.4 mM inorganic SO4 did not inhibit the production of SO4 from cysteine by these cells.

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