Conjugated Linoleic Acid Decreases Hepatic Stearoyl-CoA Desaturase mRNA Expression

1998 ◽  
Vol 248 (3) ◽  
pp. 817-821 ◽  
Author(s):  
Kisun N. Lee ◽  
Michael W. Pariza ◽  
James M. Ntambi
Keyword(s):  
Linoleic Acid ◽  
Mrna Expression ◽  
Conjugated Linoleic Acid ◽  
Stearoyl Coa Desaturase

The effect of Ala293Val single nucleotide polymorphism in the stearoyl-CoA desaturase gene on conjugated linoleic acid concentration in milk fat of dairy cows

2010 ◽  
Vol 90 (4) ◽  
pp. 575-584 ◽  
Author(s):  
L.A. Clark ◽  
J.M. Thomson ◽  
S.S. Moore ◽  
M. Oba
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Linoleic Acid ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Control Diet ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Desaturation Index ◽  
Stearoyl Coa Desaturase

The objectives of this study were to determine the effects of the Ala293Val single nucleotide polymorphism in stearoyl-CoA desaturase (SCD) on the concentration of cis-9, trans-11 conjugated linoleic acid (CLA) in milk fat of dairy cows, and to identify a phenotypic indicator of cows with a greater genetic capability to produce CLA that is not affected by dietary lipid content. In Study 1, 12 cows, four of each Ala293Val SCD genotype, were fed either a control or an oil-supplemented diet in a cross-over design to examine effects of diet, genotype and their interaction. The CLA concentration in milk fat was greater for cows fed the oil-supplemented (4.4%) vs. the control diet (0.6%), but was not affected by the Ala293Val SCD genotype. In Study 2, the Ala293Val SCD genotype of 215 dairy cows at a commercial dairy did not affect milk yield, composition, or CLA concentration in milk fat. However, in both studies, C14:1 desaturation index was greater for the AA genotype compared with the others. The Ala293Val SCD genotype may not affect CLA production although the SCD Ala293 allele is associated with a higher C14:1 desaturation index possibly because effects of the SCD genotype vary depending on fatty acid chain length or origin.

scholarly journals Effects of trans-10, cis-12 conjugated linoleic acid on the expression of uncoupling proteins in hamsters fed an atherogenic diet

2007 ◽  
Vol 97 (6) ◽  
pp. 1074-1082 ◽  
Author(s):  
Joan Ribot ◽  
Maria P. Portillo ◽  
Catalina Picó ◽  
M. Teresa Macarulla ◽  
Andreu Palou
Keyword(s):  
Skeletal Muscle ◽  
Linoleic Acid ◽  
Mrna Expression ◽  
Conjugated Linoleic Acid ◽  
Atherogenic Diet ◽  
High Dose ◽  
Mrna Levels ◽  
Fat Depots ◽  
Brown Adipose ◽  
Cpt I

It is known that conjugated linoleic acid (CLA) feeding decreases body adiposity but the mechanisms involved are not clear. The aim of this study was to analyse whether alterations in uncoupling protein (UCP) expression in white and brown adipose tissues (WAT and BAT, respectively) and in skeletal muscle may be responsible for the effect of trans-10, cis-12 CLA on the size of body fat depots in hamsters. Animals were divided into three groups and fed an atherogenic diet with different amounts of trans-10, cis-12 CLA (0 control, 0·5, or 1 g/100 g diet) for 6 weeks. CLA feeding reduced adipose depot weights, but had no effect on body weight. Leptin mRNA expression decreased in both subcutaneous and perirenal WAT depots, in accordance with lower adiposity, whereas resistin mRNA expression was not changed. Animals fed CLA had lower UCP1 mRNA levels in BAT (both doses of CLA) and in perirenal WAT (the low dose), and lower UCP3 mRNA levels in subcutaneous WAT (the high dose). UCP2 mRNA expression in WAT was not significantly affected by CLA feeding. Animals fed the high dose of CLA showed increased UCP3 and carnitine palmitoyl transferase-I (CPT-I) mRNA expression levels in skeletal muscle. In summary, induction of UCP1 or UCP2 in WAT and BAT is not likely to be responsible for the fat-reduction action of CLA, but the increased expression of UCP3 in skeletal muscle, together with a higher expression of CPT-I, may explain the previously reported effects of dietary CLA in lowering adiposity and increasing fatty acid oxidation by skeletal muscle.

scholarly journals Trans-10, cis-12-conjugated linoleic acid increases phagocytosis of porcine peripheral blood polymorphonuclear cells in vitro

2007 ◽  
Vol 97 (1) ◽  
pp. 117-125 ◽  
Author(s):  
Ji-Houn Kang ◽  
Geun-Shik Lee ◽  
Eui-Bae Jeung ◽  
Mhan-Pyo Yang
Keyword(s):  
Linoleic Acid ◽  
Mrna Expression ◽  
Conjugated Linoleic Acid ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Culture Supernatant ◽  
Supernatant Fraction ◽  
Polymorphonuclear Cells ◽  
Phagocytic Capacity ◽  
Tnf Α

Trans-10, cis-12-conjugated linoleic acid (t10c12-CLA) has been shown to alter immune function. PPARγ has been shown to potentially play an important role in regulating inflammatory and immune responses by modulating the activity of monocytes and macrophages. Previous studies have indicated that the phagocytic capacity of porcine peripheral blood polymorphonuclear cells (PMN) was enhanced by the culture supernatant fraction from t10c12-CLA-stimulated porcine peripheral blood mononuclear cells (PBMC) but not by t10c12-CLA itself. In the present study, we examined the effects of t10c12-CLA on PPARγ and TNF-α expression of porcine PBMC and the phagocytic capacity of PMN. t10c12-CLA increased TNF-α mRNA expression and production by PBMC. The phagocytic capacity of porcine PMN was enhanced by either culture supernatant fraction from PBMC treated with t10c12-CLA or recombinant porcine (rp) TNF-α. Anti-rpTNF-α polyclonal antibody inhibited the enhancement of PMN phagocytic capacity. t10c12-CLA also up regulated PPARγ mRNA expression in porcine PBMC. Bisphenol A diglycidyl ether, a PPARγ antagonist, not only completely negated the t10c12-CLA-stimulating effects on TNF-α expression and production by porcine PBMC, but also decreased the enhancement of PMN phagocytic capacity by the t10c12-CLA-stimulated porcine PBMC culture supernatant fraction. These results suggest that t10c12-CLA has an immunostimulating effect on porcine PMN phagocytic capacity, which is mediated by TNF-α from PBMC via a PPARγ-dependent pathway.

Effects of dietary conjugated linoleic acid on cytotoxicity of peripheral blood lymphocytes in piglets

2016 ◽  
Vol 96 (2) ◽  
pp. 154-160
Author(s):  
Yongxiang Liu ◽  
Yanli Liu ◽  
Rongchao Qiu ◽  
Dongfeng Jiang
Keyword(s):  
Linoleic Acid ◽  
Mrna Expression ◽  
Conjugated Linoleic Acid ◽  
Peripheral Blood ◽  
Control Diet ◽  
Peripheral Blood Lymphocytes ◽  
Control Group ◽  
Large White ◽  
Blood Lymphocytes ◽  
Dietary Conjugated Linoleic Acid

The aim of this study was to investigate the effects of dietary conjugated linoleic acid (CLA) on the cytotoxicity of peripheral blood lymphocytes in piglets. A total of forty 21-d-old Duroc × Large White × Landrace weaned piglets were randomly allocated into two treatment groups and fed with control diet or 2% CLA diet for 6 weeks. Dietary CLA significantly increased the number of peripheral blood CD8+ T lymphocytes (P < 0.05). Compared with the control group, cytotoxicity, perforin, and granzyme B mRNA expression of peripheral blood lymphocytes in piglets from the CLA-supplementation group increased by 29.98% (P < 0.01), 32.87% (P < 0.01), and 43.36% (P < 0.05), respectively. The piglets on the CLA diet had higher mRNA expression of Lck and Fyn in peripheral blood lymphocytes (P < 0.05). The content of C16:0 significantly increased in piglets from the dietary CLA-supplementation group (P < 0.05), whereas the content of C18:1 significantly decreased (P < 0.05). The CLA content of peripheral blood lymphocytes in piglets in the CLA diet group ranged up to 0.24% (P < 0.01). These results suggest that dietary CLA enhanced cytotoxicity of peripheral blood lymphocytes perhaps through changing its fatty acid composition.

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