Generation of a Tropoelastin mRNA Variant by Alternative Polyadenylation Site Selection in Sun-Damaged Human Skin and Ultraviolet B-Irradiated Fibroblasts

1998 ◽  
Vol 246 (1) ◽  
pp. 217-221 ◽  
Author(s):  
Elaine Schwartz ◽  
Joel M. Gelfand ◽  
James C. Mauch ◽  
Lorraine H. Kligman
Keyword(s):  
Human Skin ◽  
Site Selection ◽  
Alternative Polyadenylation ◽  
Ultraviolet B ◽  
Polyadenylation Site ◽  
Mrna Variant

Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection

Biochemistry ◽  
1990 ◽  
Vol 29 (22) ◽  
pp. 5293-5299 ◽  
Author(s):  
B. Bousquet-Lemercier ◽  
S. Pol ◽  
M. Pave-Preux ◽  
J. Hanoune ◽  
R. Barouki
Keyword(s):  
Site Selection ◽  
Aspartate Aminotransferase ◽  
Human Liver ◽  
Alternative Polyadenylation ◽  
Polyadenylation Site

Activation of HIV in Human Skin by Ultraviolet B Radiation and its Inhibition by NFκB Blocking Agents¶

2001 ◽  
Vol 74 (6) ◽  
pp. 805 ◽  
Author(s):  
Joan Breuer-McHam ◽  
Eric Simpson ◽  
Irene Dougherty ◽  
Makoto Bonkobara ◽  
Kiyoshi Ariizumi ◽  
...  
Keyword(s):  
Human Skin ◽  
Ultraviolet B ◽  
Ultraviolet B Radiation ◽  
Blocking Agents

scholarly journals α-MSH Counteracts the Suppressive Effect of Ultraviolet B on Nrf1/2 and Nrf-Dependent Gene Expression in Human Skin

2009 ◽  
Vol 94 (5) ◽  
pp. 1835-1835
Author(s):  
Agatha Kokot ◽  
Dieter Metze ◽  
Nicolas Mouchet ◽  
Marie-Dominique Galibert ◽  
Meinhard Schiller ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Skin ◽  
Suppressive Effect ◽  
Ultraviolet B

scholarly journals Alternative Polyadenylation of Human Bocavirus at Its 3′ End Is Regulated by Multiple Elements and Affects Capsid Expression

2016 ◽  
Vol 91 (3) ◽  
Author(s):  
Sujuan Hao ◽  
Junmei Zhang ◽  
Zhen Chen ◽  
Huanzhou Xu ◽  
Hanzhong Wang ◽  
...  
Keyword(s):  
Nonstructural Protein ◽  
Infectious Clone ◽  
Alternative Polyadenylation ◽  
Polyadenylation Site ◽  
Human Bocavirus ◽  
Loop Structure ◽  
Stem Loop ◽  
Stem Loop Structure ◽  
Mrna Transcripts ◽  
The Right

ABSTRACT Alternative processing of human bocavirus (HBoV) P5 promoter-transcribed RNA is critical for generating the structural and nonstructural protein-encoding mRNA transcripts. The regulatory mechanism by which HBoV RNA transcripts are polyadenylated at proximal [(pA)p] or distal [(pA)d] polyadenylation sites is still unclear. We constructed a recombinant HBoV infectious clone to study the alternative polyadenylation regulation of HBoV. Surprisingly, in addition to the reported distal polyadenylation site, (pA)d, a novel distal polyadenylation site, (pA)d2, which is located in the right-end hairpin (REH), was identified during infectious clone transfection or recombinant virus infection. (pA)d2 does not contain typical hexanucleotide polyadenylation signal, upstream elements (USE), or downstream elements (DSE) according to sequence analysis. Further study showed that HBoV nonstructural protein NS1, REH, and cis elements of (pA)d were necessary and sufficient for efficient polyadenylation at (pA)d2. The distance and sequences between (pA)d and (pA)d2 also played a key role in the regulation of polyadenylation at (pA)d2. Finally, we demonstrated that efficient polyadenylation at (pA)d2 resulted in increased HBoV capsid mRNA transcripts and protein translation. Thus, our study revealed that all the bocaviruses have distal poly(A) signals on the right-end palindromic terminus, and alternative polyadenylation at the HBoV 3′ end regulates its capsid expression. IMPORTANCE The distal polyadenylation site, (pA)d, of HBoV is located about 400 nucleotides (nt) from the right-end palindromic terminus, which is different from those of bovine parvovirus (BPV) and canine minute virus (MVC) in the same genus whose distal polyadenylation is located in the right-end stem-loop structure. A novel polyadenylation site, (pA)d2, was identified in the right-end hairpin of HBoV during infectious clone transfection or recombinant virus infection. Sequence analysis showed that (pA)d2 does not contain typical polyadenylation signals, and the last 42 nt form a stem-loop structure which is almost identical to that of MVC. Further study showed that NS1, REH, and cis elements of (pA)d are required for efficient polyadenylation at (pA)d2. Polyadenylation at (pA)d2 enhances capsid expression. Our study demonstrates alternative polyadenylation at the 3′ end of HBoV and suggests an additional mechanism by which capsid expression is regulated.

scholarly journals Characterization of a Human Skin Equivalent Model to Study the Effects of Ultraviolet B Radiation on Keratinocytes

2014 ◽  
Vol 20 (7) ◽  
pp. 588-598 ◽  
Author(s):  
Tara L. Fernandez ◽  
Derek R. Van Lonkhuyzen ◽  
Rebecca A. Dawson ◽  
Michael G. Kimlin ◽  
Zee Upton
Keyword(s):  
Human Skin ◽  
Ultraviolet B ◽  
Skin Equivalent ◽  
Equivalent Model ◽  
Ultraviolet B Radiation ◽  
Human Skin Equivalent Model ◽  
Human Skin Equivalent
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