Glyphosate Is an Inhibitor of Plant Cytochrome P450: Functional Expression ofThlaspi arvensaeCytochrome P45071B1/Reductase Fusion Protein inEscherichia coli

1998 ◽  
Vol 244 (1) ◽  
pp. 110-114 ◽  
Author(s):  
D.C. Lamb ◽  
D.E. Kelly ◽  
S.Z. Hanley ◽  
Z. Mehmood ◽  
S.L. Kelly
Keyword(s):  
Cytochrome P450 ◽  
Fusion Protein ◽  
Functional Expression ◽  
Inescherichia Coli

scholarly journals Effect of Hemodialysis on Hepatic Cytochrome P450 Functional Expression

2008 ◽  
Vol 108 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Josée Michaud ◽  
Thomas D. Nolin ◽  
Judith Naud ◽  
Mélina Dani ◽  
Jean-Philippe Lafrance ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Functional Expression ◽  
Hepatic Cytochrome

Refolding of a Recombinant Collagen-Targeted TGF-β2 Fusion Protein Expressed inEscherichia coli

1997 ◽  
Vol 11 (2) ◽  
pp. 169-178 ◽  
Author(s):  
Bo Han ◽  
Frederick L. Hall ◽  
Marcel E. Nimni
Keyword(s):  
Fusion Protein ◽  
Inescherichia Coli

Cortisol production in sheep is influenced by the functional expression of two cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17) isoformsl

2013 ◽  
Vol 91 (3) ◽  
pp. 1193-1206 ◽  
Author(s):  
D. Hough ◽  
S. W. P. Cloete ◽  
K. Storbeck ◽  
A. C. Swart ◽  
P. Swart
Keyword(s):  
Cytochrome P450 ◽  
Functional Expression

Purification and Characterization of Recombinant Osteocalcin Fusion Protein Expressed inEscherichia coli

1996 ◽  
Vol 8 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Sanna-Maria Käkönen ◽  
Jukka Hellman ◽  
Kim Pettersson ◽  
Timo Lövgren ◽  
Matti Karp
Keyword(s):  
Fusion Protein ◽  
Inescherichia Coli ◽  
Purification And Characterization

scholarly journals Efficient production of vindoline from tabersonine by metabolically engineered Saccharomyces cerevisiae

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Tengfei Liu ◽  
Ying Huang ◽  
Lihong Jiang ◽  
Chang Dong ◽  
Yuanwei Gou ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
De Novo ◽  
Indole Alkaloid ◽  
Functional Expression ◽  
High Yield ◽  
Efficient Production ◽  
Cytochrome P450 Enzymes ◽  
Copy Numbers ◽  
Yield Production ◽  
Engineered Saccharomyces Cerevisiae

AbstractVindoline is a plant derived monoterpene indole alkaloid (MIA) with potential therapeutic applications and more importantly serves as the precursor to vinblastine and vincristine. To obtain a yeast strain for high yield production of vindoline from tabersonine, multiple metabolic engineering strategies were employed via the CRISPR/Cas9 mediated multiplex genome integration technology in the present study. Through increasing and tuning the copy numbers of the pathway genes, pairing cytochrome P450 enzymes (CYPs) with appropriate cytochrome P450 reductases (CPRs), engineering the microenvironment for functional expression of CYPs, enhancing cofactor supply, and optimizing fermentation conditions, the production of vindoline was increased to a final titer as high as ∼16.5 mg/L, which is more than 3,800,000-fold higher than the parent strain and the highest tabersonine to vindoline conversion yield ever reported. This work represents a key step of the engineering efforts to establish de novo biosynthetic pathways for vindoline, vinblastine, and vincristine.

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