Cortisol production in sheep is influenced by the functional expression of two cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17) isoformsl

2013 ◽  
Vol 91 (3) ◽  
pp. 1193-1206 ◽  
Author(s):  
D. Hough ◽  
S. W. P. Cloete ◽  
K. Storbeck ◽  
A. C. Swart ◽  
P. Swart
Keyword(s):  
Cytochrome P450 ◽  
Functional Expression

scholarly journals Effect of Hemodialysis on Hepatic Cytochrome P450 Functional Expression

2008 ◽  
Vol 108 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Josée Michaud ◽  
Thomas D. Nolin ◽  
Judith Naud ◽  
Mélina Dani ◽  
Jean-Philippe Lafrance ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Functional Expression ◽  
Hepatic Cytochrome

scholarly journals Efficient production of vindoline from tabersonine by metabolically engineered Saccharomyces cerevisiae

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Tengfei Liu ◽  
Ying Huang ◽  
Lihong Jiang ◽  
Chang Dong ◽  
Yuanwei Gou ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
De Novo ◽  
Indole Alkaloid ◽  
Functional Expression ◽  
High Yield ◽  
Efficient Production ◽  
Cytochrome P450 Enzymes ◽  
Copy Numbers ◽  
Yield Production ◽  
Engineered Saccharomyces Cerevisiae

AbstractVindoline is a plant derived monoterpene indole alkaloid (MIA) with potential therapeutic applications and more importantly serves as the precursor to vinblastine and vincristine. To obtain a yeast strain for high yield production of vindoline from tabersonine, multiple metabolic engineering strategies were employed via the CRISPR/Cas9 mediated multiplex genome integration technology in the present study. Through increasing and tuning the copy numbers of the pathway genes, pairing cytochrome P450 enzymes (CYPs) with appropriate cytochrome P450 reductases (CPRs), engineering the microenvironment for functional expression of CYPs, enhancing cofactor supply, and optimizing fermentation conditions, the production of vindoline was increased to a final titer as high as ∼16.5 mg/L, which is more than 3,800,000-fold higher than the parent strain and the highest tabersonine to vindoline conversion yield ever reported. This work represents a key step of the engineering efforts to establish de novo biosynthetic pathways for vindoline, vinblastine, and vincristine.

Functional expression and purification of CYP93C20, a plant membrane-associated cytochrome P450 from Medicago truncatula

2018 ◽  
Vol 150 ◽  
pp. 44-52 ◽  
Author(s):  
Zhenzhan Chang ◽  
Xiaoqiang Wang ◽  
Risheng Wei ◽  
Zhouying Liu ◽  
Hong Shan ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Medicago Truncatula ◽  
Functional Expression ◽  
Expression And Purification

Cloning and functional expression of a novel marmoset cytochrome P450 2D enzyme, CYP2D30: comparison with the known marmoset CYP2D19

2004 ◽  
Vol 68 (1) ◽  
pp. 165-175 ◽  
Author(s):  
Hiroyuki Hichiya ◽  
Shino Kuramoto ◽  
Shigeo Yamamoto ◽  
Sumio Shinoda ◽  
Nobumitsu Hanioka ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Functional Expression

scholarly journals Comparison of Two Vectors for Functional Expression of a Bacterial Cytochrome P450 Gene inEscherichia coliUsingCYP153Genes

2009 ◽  
Vol 73 (8) ◽  
pp. 1825-1830 ◽  
Author(s):  
Naoya FUJITA ◽  
Futoshi SUMISA ◽  
Kazutoshi SHINDO ◽  
Hiroki KABUMOTO ◽  
Akira ARISAWA ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Functional Expression ◽  
Cytochrome P450 Gene ◽  
P450 Gene
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