Synergism of the ATF/CRE Site and GC Box in the Housekeeping Na,K-ATPase α1 Subunit Gene Is Essential for Constitutive Expression

1997 ◽  
Vol 241 (1) ◽  
pp. 169-174 ◽  
Author(s):  
Makoto Kobayashi ◽  
Kiyoshi Kawakami
Keyword(s):  
Constitutive Expression ◽  
Subunit Gene ◽  
Gc Box ◽  
Α1 Subunit

Genetic and Developmental Characterization of Dmca1D, a Calcium Channel α1 Subunit Gene in Drosophila melanogaster

Genetics ◽  
1998 ◽  
Vol 148 (3) ◽  
pp. 1159-1169
Author(s):  
Daniel F Eberl ◽  
Dejian Ren ◽  
Guoping Feng ◽  
Lori J Lorenz ◽  
David Van Vactor ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Calcium Channel ◽  
Functional Significance ◽  
Transmembrane Domain ◽  
Vital Role ◽  
Subunit Gene ◽  
Channel Diversity ◽  
Domain I ◽  
Α1 Subunit

Abstract To begin unraveling the functional significance of calcium channel diversity, we identified mutations in Dmca1D, a Drosophila calcium channel α1 subunit cDNA that we recently cloned. These mutations constitute the l(2)35Fa lethal locus, which we rename Dmca1D. A severe allele, Dmca1DX10, truncates the channel after the IV-S4 transmembrane domain. These mutants die as late embryos because they lack vigorous hatching movements. In the weaker allele, Dmca1DAR66, a cysteine in transmembrane domain I-S1 is changed to tyrosine. Dmca1DAR66 embryos hatch but pharate adults have difficulty eclosing. Those that do eclose have difficulty in fluid-filling of the wings. These studies show that this member of the calcium channel α1 subunit gene family plays a nonredundant, vital role in larvae and adults.

scholarly journals ADrosophilaCalcium Channel α1 Subunit Gene Maps to a Genetic Locus Associated with Behavioral and Visual Defects

1996 ◽  
Vol 16 (24) ◽  
pp. 7868-7879 ◽  
Author(s):  
Lee A. Smith ◽  
XinJing Wang ◽  
Alexandre A. Peixoto ◽  
Eric K. Neumann ◽  
Linda M. Hall ◽  
...  
Keyword(s):  
Genetic Locus ◽  
Subunit Gene ◽  
Visual Defects ◽  
Gene Maps ◽  
Α1 Subunit

scholarly journals Cloning, mapping, and developmental expression of a sixth zebrafish Na,K-ATPase α1 subunit gene (atp1a1a.5)

2002 ◽  
Vol 119 ◽  
pp. S211-S214 ◽  
Author(s):  
Brian Blasiole ◽  
Victor Canfield ◽  
Agnes Degrave ◽  
Christine Thisse ◽  
Bernard Thisse ◽  
...  
Keyword(s):  
Developmental Expression ◽  
Subunit Gene ◽  
Α1 Subunit

Loss-of-function mutations in a calcium-channel α1-subunit gene in Xp11.23 cause incomplete X-linked congenital stationary night blindness

10.1038/947 ◽  
1998 ◽  
Vol 19 (3) ◽  
pp. 264-267 ◽  
Author(s):  
N. Torben Bech-Hansen ◽  
Margaret J. Naylor ◽  
Tracy A. Maybaum ◽  
William G. Pearce ◽  
Ben Koop ◽  
...  
Keyword(s):  
Calcium Channel ◽  
Night Blindness ◽  
Congenital Stationary Night Blindness ◽  
Loss Of Function ◽  
Subunit Gene ◽  
Α1 Subunit

scholarly journals Different factors bind to the regulatory region of the Na+,K+-ATPase α1-subunit gene during the cell cycle

FEBS Letters ◽  
1993 ◽  
Vol 335 (2) ◽  
pp. 251-254 ◽  
Author(s):  
Kiyoshi Kawakami ◽  
Ken Yanagisawa ◽  
Yuko Watanabe ◽  
Shin-ichi Tominaga ◽  
Kei Nagano
Keyword(s):  
Cell Cycle ◽  
Regulatory Region ◽  
Subunit Gene ◽  
Α1 Subunit

scholarly journals Overlapping antioxidant response element and PMA responseelement sequences mediate basal and β-naphthoflavone-induced expression of the human γ-glutamylcysteine synthetase catalytic subunit gene

1998 ◽  
Vol 332 (2) ◽  
pp. 373-381 ◽  
Author(s):  
Angela C. WILD ◽  
Jerry J. GIPP ◽  
R. Timothy MULCAHY
Keyword(s):  
Hepg2 Cells ◽  
Constitutive Expression ◽  
Antioxidant Response ◽  
Regulatory Subunit ◽  
Antioxidant Response Element ◽  
Induced Expression ◽  
Response Element ◽  
Relative Significance ◽  
Glutamylcysteine Synthetase ◽  
Gc Box

γ-Glutamylcysteine synthetase (GCS), the rate-limiting enzyme in the de novo synthesis of GSH, is a heterodimer, consisting of a catalytic (GCSh) and a regulatory subunit (GCSl). We previously demonstrated that the constitutive and β-naphthoflavone (β-NF)-induced expression of the GCSh gene is mediated by a distal antioxidant response element (ARE), ARE4, located 3.1 kb upstream of the transcriptional start site [Mulcahy, Wartman, Bailey and Gipp (1997) J. Biol. Chem. 272, 7445–7454]. ARE4 consists of a consensus ARE sequence (5´-GTGACTCAGCG-3´) containing an embedded PMA-responsive element (TRE, underlined). The relative significance of the two overlapping response elements to constitutive and β-NF-induced expression of the GCSh gene was determined by mutational analyses. The internal activator protein-1 (AP-1)-binding sequence mediated constitutive expression of promoter/reporter transgenes, but was not required for β-NF responsiveness. In gel-shift experiments, the TRE was necessary for binding of proteins from nuclear extracts prepared from untreated HepG2 cells. In contrast, induction by β-NF was dependent on an intact ARE sequence, particularly the terminal GC box of ARE4. The GC box of ARE4 was shown to be essential for both basal and β-NF-induced expression of reporter constructs. This element also influenced binding of nuclear proteins to ARE4, specifically in extracts isolated from β-NF-treated HepG2 cells. Because previous studies indicated that ARE4 may co-operate with a separate putative ARE, the role of the neighbouring sequence (ARE3), located 34 bases downstream of ARE4, was also evaluated. Mutation of this element within a GCSh promoter/reporter did not modify the basal or β-NF-induced expression of the transgene, demonstrating that ARE3 does not influence the constitutive or β-NF-induced expression of the GCSh gene.

Isolation and sequence analysis of the chicken GABAA receptor α1-subunit gene promoter

Gene ◽  
1995 ◽  
Vol 153 (2) ◽  
pp. 243-247 ◽  
Author(s):  
Alan N. Bateson ◽  
Andreas Ultsch ◽  
Mark G. Darlison
Keyword(s):  
Sequence Analysis ◽  
Gabaa Receptor ◽  
Gene Promoter ◽  
Subunit Gene ◽  
Α1 Subunit
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