Insensitivity of Cathepsin D Gene to Estradiol in Endometrial Cells Is Determined by the Sequence of Its Estrogen Responsive Element

1994 ◽  
Vol 203 (1) ◽  
pp. 711-718 ◽  
Author(s):  
F. Miralles ◽  
C. Gaudelet ◽  
V. Cavailles ◽  
H. Rochefort ◽  
P. Augereau
Keyword(s):  
Cathepsin D ◽  
Endometrial Cells ◽  
Estrogen Responsive Element ◽  
Responsive Element

scholarly journals Characterization of the proximal estrogen-responsive element of human cathepsin D gene.

1994 ◽  
Vol 8 (6) ◽  
pp. 693-703 ◽  
Author(s):  
P Augereau ◽  
F Miralles ◽  
V Cavaillès ◽  
C Gaudelet ◽  
M Parker ◽  
...  
Keyword(s):  
Cathepsin D ◽  
Estrogen Responsive Element ◽  
Human Cathepsin ◽  
Responsive Element

scholarly journals Real-Time Challenging of ERα Y537S Mutant Transcriptional Activity in Living Cells

Endocrines ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 54-64
Author(s):  
Manuela Cipolletti ◽  
Sara Pescatori ◽  
Filippo Acconcia
Keyword(s):  
Cell Line ◽  
Transcriptional Activity ◽  
Estrogen Receptor Α ◽  
Living Cells ◽  
Patient Treatment ◽  
Estrogen Responsive Element ◽  
Specific Proteins ◽  
Responsive Element ◽  
Mcf 7

Metastatic estrogen receptor α (ERα)-expressing breast cancer (BC) occurs after prolonged patient treatment with endocrine therapy (ET) (e.g., aromatase inhibitors—AI; 4OH-tamoxifen—4OH-Tam). Often these metastatic BCs express a mutated ERα variant (e.g., Y537S), which is transcriptionally hyperactive, sustains uncontrolled proliferation, and renders tumor cells insensitive to ET drugs. Therefore, new molecules blocking hyperactive Y537S ERα mutation transcriptional activity are requested. Here we generated an MCF-7 cell line expressing the Y537S ERα mutation stably expressing an estrogen-responsive element (ERE) promoter, which activity can be monitored in living cells. Characterization of this cell line shows both hyperactive basal transcriptional activity with respect to normal MCF-7 cells, which stably express the same ERE-based promoter and a decreased effect of selective ER downregulators (SERDs) in reducing Y537S ERα mutant transcriptional activity with respect to wild type ERα transcriptional activity. Kinetic profiles of Y537S ERα mutant-based transcription produced by both drugs inducing receptor degradation and siRNA-mediated depletion of specific proteins (e.g., FOXA1 and caveolin1) reveals biphasic dynamics of the inhibition of the receptor-regulated transcriptional effects. Overall, we report a new model where to study the behavior of the Y537S ERα mutant that can be used for the identification of new targets and pathways regulating the Y537S ERα transcriptional activity.

An estrogen-responsive element derived from the 5′ flanking region of the Xenopus vitellogenin A2 gene functions in transfected human cells

Cell ◽  
1986 ◽  
Vol 46 (7) ◽  
pp. 1053-1061 ◽  
Author(s):  
Ludger Klein-Hitpaß ◽  
Marina Schorpp ◽  
Ulrike Wagner ◽  
Gerhart U. Ryffel
Keyword(s):  
Human Cells ◽  
Estrogen Responsive Element ◽  
Gene Functions ◽  
Flanking Region ◽  
Responsive Element
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