Peptide growth factors elicit estrogen receptor-dependent transcriptional activation of an estrogen-responsive element

1993 ◽  
Vol 7 (8) ◽  
pp. 992-998 ◽  
Author(s):  
D. M. Ignar-Trowbridge
Keyword(s):  
Estrogen Receptor ◽  
Growth Factors ◽  
Transcriptional Activation ◽  
Peptide Growth Factors ◽  
Estrogen Responsive Element ◽  
Responsive Element

scholarly journals A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor

1988 ◽  
Vol 16 (2) ◽  
pp. 647-663 ◽  
Author(s):  
Ludger Klein-Hitpass ◽  
Gerhart U. Ryffel ◽  
Ellen Heitlinger ◽  
Andrew C.B. Cato
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Responsive Element ◽  
Responsive Element

scholarly journals In VitroBinding of the Purified Hormone-Binding Subunit of the Estrogen Receptor to Oligonucleotides Containing Natural or Modified Sequences of an Estrogen-Responsive Element

1991 ◽  
Vol 5 (4) ◽  
pp. 555-563 ◽  
Author(s):  
Nicola Medici ◽  
Vincenzo Nigro ◽  
Ciro Abbondanza ◽  
Bruno Moncharmont ◽  
Anna Maria Molinari ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Hormone Binding ◽  
Estrogen Responsive Element ◽  
Responsive Element ◽  
Binding Subunit

Characterization of an estrogen-responsive element implicated in regulation of the rainbow trout estrogen receptor gene

1995 ◽  
Vol 15 (1) ◽  
pp. 37-47 ◽  
Author(s):  
Y Le Dréan ◽  
G Lazennec ◽  
L Kern ◽  
D Saligaut ◽  
F Pakdel ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Rainbow Trout ◽  
Receptor Gene ◽  
Expression System ◽  
Consensus Sequence ◽  
Gene Promoter ◽  
Dnase I Footprinting ◽  
Estrogen Responsive Element ◽  
Responsive Element ◽  
Simplex Virus

ABSTRACT We previously reported that the expression of the rainbow trout estrogen receptor (rtER) gene is markedly increased by estradiol (E2). In this paper, we have used transient transfection assays with reporter plasmids expressing chloramphenicol acetyl transferase (CAT), linked to 5′ flanking regions of the rtER gene promoter, to identify cis-elements responsible for E2 inducibility. Deletion analysis localized an estrogen-responsive element (ERE), at position +242, with one mutation on the first base compared with the consensus sequence. This element confers estrogen responsiveness to CAT reporter linked to both the herpes simplex virus thymidine kinase promoter and the homologous rtER promoter. Moreover, using a 0·2 kb fragment of the rtER promoter encompassing the ERE and the rtER DNA binding domain obtained from a bacterial expression system, DNase I footprinting experiments demonstrated a specific protection covering 20 bp (+240/+260) containing the ERE sequence. Based on these studies, we believe that this ERE sequence, identified in the rtER gene promoter, may be a major cis-acting element involved in the regulation of the gene by estrogen.

scholarly journals Neuropeptide B mediates female sexual receptivity in medaka fish, acting in a female-specific but reversible manner

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Towako Hiraki-Kajiyama ◽  
Junpei Yamashita ◽  
Keiko Yokoyama ◽  
Yukiko Kikuchi ◽  
Mikoto Nakajo ◽  
...  
Keyword(s):  
Mating Behavior ◽  
Transcriptional Activation ◽  
Sex Steroid ◽  
Critical Element ◽  
Specific Expression ◽  
Behavioral Studies ◽  
Estrogen Responsive Element ◽  
Mating Behaviors ◽  
Responsive Element ◽  
Female Specific

Male and female animals display innate sex-specific mating behaviors. In teleost fish, altering the adult sex steroid milieu can effectively reverse sex-typical mating behaviors, suggesting remarkable sexual lability of their brains as adults. In the teleost medaka, neuropeptide B (NPB) is expressed female-specifically in the brain nuclei implicated in mating behavior. Here, we demonstrate that NPB is a direct mediator of estrogen action on female mating behavior, acting in a female-specific but reversible manner. Analysis of regulatory mechanisms revealed that the female-specific expression of NPB is dependent on direct transcriptional activation by estrogen via an estrogen-responsive element and is reversed in response to changes in the adult sex steroid milieu. Behavioral studies of NPB knockouts revealed that female-specific NBP mediates female receptivity to male courtship. The female-specific NPB signaling identified herein is presumably a critical element of the neural circuitry underlying sexual dimorphism and lability of mating behaviors in teleosts.

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