Detection of Genetic Point Mutations by Peptide Nucleic Acid-Mediated Polymerase Chain Reaction Clamping Using Paraffin-Embedded Specimens

2000 ◽  
Vol 285 (1) ◽  
pp. 169-172 ◽  
Author(s):  
Yvonne Myal ◽  
Anne Blanchard ◽  
Peter Watson ◽  
Michael Corrin ◽  
Robert Shiu ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Point Mutations ◽  
Chain Reaction ◽  
Polymerase Chain

Investigation on Natural Diets of Larval Marine Animals Using Peptide Nucleic Acid-Directed Polymerase Chain Reaction Clamping

2010 ◽  
Vol 13 (2) ◽  
pp. 305-313 ◽  
Author(s):  
Seinen Chow ◽  
Sayaka Suzuki ◽  
Tadashi Matsunaga ◽  
Shane Lavery ◽  
Andrew Jeffs ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Chain Reaction ◽  
Marine Animals ◽  
Polymerase Chain

A peptide nucleic acid (PNA)-mediated polymerase chain reaction clamping allows the selective inhibition of the ERVWE1 gene amplification

2014 ◽  
Vol 28 (5-6) ◽  
pp. 237-241 ◽  
Author(s):  
Grzegorz Machnik ◽  
Krzysztof Łabuzek ◽  
Estera Skudrzyk ◽  
Piotr Rekowski ◽  
Jarosław Ruczyński ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Gene Amplification ◽  
Peptide Nucleic Acid ◽  
Selective Inhibition ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Simple and rapid detection of common fetal aneuploidies using peptide nucleic acid probe-based real-time polymerase chain reaction

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Subeen Hong ◽  
Seung Mi Lee ◽  
Sohee Oh ◽  
So Yeon Kim ◽  
Young Mi Jung ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Peptide Nucleic Acid ◽  
Melting Curve ◽  
Peak Height ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Polymerase Chain

AbstractTo examine the detection performance of a peptide nucleic acid (PNA) probe-based real-time time polymerase chain reaction (PCR) assay to detect common aneuploidies. Using amniotic fluid samples, PNA probe based real-time PCR (Patio DEP Detection Kit; SeaSun Biomaterials, Korea) assay was performed. PNA probe was designed to hybridize to similar sequences located on different segments of target chromosomes (21, 18, and 13) and a reference chromosome. Amplification of target sequences and melting curve analysis was performed. When analyzing the melting curve, the ratio of the peak height of the target and reference chromosome was calculated and determined as aneuploidy if the ratio of peak height was abnormal. All the results from the PNA probe-based real-time PCR and melting curve analyses were compared to those from conventional karyotyping. Forty-two cases with common aneuploidies (24 of trisomy 21, 12 of trisomy 18, and 6 of trisomy 13) and 131 cases with normal karyotype were analyzed. When comparing the karyotyping results, the sensitivity and specificity of the PNA probe-based real-time PCR assay were both 100%. The level of agreement was almost perfect (k = 1.00). PNA real-time PCR assay is a rapid and easy method for detecting common aneuploidies.

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