A Simple Assay Method for Bacterial Binding to Glycosphingolipids on a Polyvinylidene Difluoride Membrane after Thin-Layer Chromatography Blotting andin SituMass Spectrometric Analysis of the Ligands

1996 ◽  
Vol 236 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Tomomi Isobe ◽  
Masaharu Naiki ◽  
Shizuo Handa ◽  
Takao Taki
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Assay Method ◽  
Spectrometric Analysis ◽  
Polyvinylidene Difluoride ◽  
Bacterial Binding ◽  
Layer Chromatography

scholarly journals Antimicrobial Evaluation of Latex and TLC Fractions from the Leaves of Aloe adigratana Reynolds

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Demoze Asmerom ◽  
Gebremedhin Solomon Hailu ◽  
Ebrahim M. Yimer ◽  
Helen Bitew ◽  
Getu Kahsay
Keyword(s):  
Staphylococcus Aureus ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antimicrobial Agents ◽  
Antimicrobial Effect ◽  
Candida Krusei ◽  
Assay Method ◽  
Bacterial Strains ◽  
Microbial Strains ◽  
Layer Chromatography

Background. The highest prevalence and emergence of microbial infections coupled with the threat of antimicrobial resistance constitute a global concern, which entails searching for novel antimicrobial agents. Medicinal plants are among the major sources of medicines for novel drug discovery. Aloe adigratana is one of the endemic Aloe species in Ethiopia where the leaf latex of the plant is traditionally used for the treatment of various pathogenic conditions such as wound, dandruff, malaria, and diabetes. In spite of such claims, there was no scientific study done so far. The aim of the current study was, therefore, to evaluate the antimicrobial effect of leaf latex of A. adigratana and its thin layer chromatography (TLC) fractions. Methods. Thin layer chromatography (TLC) separation was employed for isolation of bioactive compounds. Agar well diffusion and microdilution assay method were used to evaluate the antimicrobial actions of the leaf latex and TLC fractions against six bacterial strains and four Candida species of reference and clinical isolate microbial strains. Results. Three major fractions, AA01, AA02, and AA03, were identified by TLC. Among the tested microbial strains, the reference strain of Staphylococcus aureus ATCC 29213 (MIC = 0.06 mg/mL) and clinical Candida krusei 242/18 (MIC = 0.14 mg/mL) exhibited higher susceptibility towards AA02, while reference strains of Klebsiella pneumoniae ATCC 700603 (MIC = 0.19 mg/mL) revealed the highest susceptibility towards AA01. The leaf latex displayed the highest activity against Staphylococcus aureus ATCC 29213 and clinical Candida krusei 242/18 with a MIC value of 0.19 mg/mL. Conclusion. The leaf latex and TLC fractions were found to be active against the tested bacterial and Candida species. Therefore, this finding supports the traditional claim of Aloe adigratana and the need for characterization of the TLC fractions to provide as lead compounds for further comprehensive antibacterial and antifungal activities.

scholarly journals Cytotoxic Activity of Flavonoid Compounds Isolated from Tetrameles Nudliflora Leaves

2020 ◽  
Vol 23 (2) ◽  
pp. 81
Author(s):  
Megawati Megawati ◽  
Akhmad Darmawan ◽  
Sofa Fajriah
Keyword(s):  
Thin Layer ◽  
Cytotoxic Activity ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Assay Method ◽  
Cytotoxic Activities ◽  
Spectroscopic Techniques ◽  
Flavonoid Compounds ◽  
Layer Chromatography ◽  
Mcf 7

Tetrameles nudliflora is one of the endemic plants in Wallace region, especially in Mekongga Forest, Southeast Sulawesi, Indonesia. As part of our project in the field of natural drug discovery based on local bioresources, this study aimed to isolate secondary metabolite compounds from the ethyl acetate fractions of the leaves of T. nudiflora and evaluate their cytotoxic activities against breast carcinoma cancer lines, MCF-7. The methods used were various chromatography techniques such as vacuum column chromatography, gravitation column chromatography, thin layer chromatography, preparative thin layer chromatography, and re-crystallization using two different organic solvents. In the present study, two flavonoid compounds, kaempferol (1) and quercetin (2) were identified. The structures of 1-2 were determined using serial spectroscopic techniques, including mass spectrometry, one- and two-dimensional nuclear magnetic resonance. Moreover, using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay method, both compounds showed in vitro cytotoxic activity IC50 of 34.162 and 133.148 μg mL-1, respectively against MCF-7 cell lines.

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