Use of Computer-Assisted Motion Analysis for Quantitative Measurements of Swimming Behavior in Peritrichously Flagellated Bacteria

1996 ◽  
Vol 235 (1) ◽  
pp. 20-25 ◽  
Author(s):  
Charles D. Amsler
1994 ◽  
Vol 40 (9) ◽  
pp. 705-711 ◽  
Author(s):  
Geralyne Lopez-de-Victoria ◽  
Donald R. Fielder ◽  
Richard K. Zimmer-Faust ◽  
Charles R. Lovell

The motility of Azospirillum lipoferum Sp59b and Azospirillum brasilense Sp7 in the presence of the aromatic compounds benzoate, protocatechuate, 4-hydroxybenzoic acid, and catechol was studied using computer-assisted video motion analysis. Increases in swimming speed with concomitant decreases in the rate of change in direction and increases in path linearity are indicative of bacterial chemoattraction. With the exception of 4-hydroxybenzoic acid, the aromatic compounds tested altered the motility patterns of both species, consistent with a chemoattraction mechanism. 4-Hydroxybenzoic acid elicited significant behavioral changes only in A. brasilense. The highest speeds were recorded for most compounds at concentrations between 1 and 10 mM for A. lipoferum and at lower concentrations for A. brasilense. Catechol, supplied at a 10 mM concentration, reduced the swimming speed of A. brasilense but not of A. lipoferum. The distinct differences in swimming behavior observed between these two species of Azospirillum in response to aromatic compounds may be of ecological significance in soils.Key words: Azospirillum spp., motility, video motion analysis, aromatic compounds.


2000 ◽  
Vol 46 (5) ◽  
pp. 279-286 ◽  
Author(s):  
Masako SATO ◽  
Ryo OHTA ◽  
Kazuyoshi WADA ◽  
Hideki MARUMO ◽  
Mariko SHIROTA ◽  
...  

10.14444/2036 ◽  
2015 ◽  
Vol 9 ◽  
pp. 36 ◽  
Author(s):  
Reginald J. Davis ◽  
David C. Lee ◽  
Chip Wade ◽  
Boyle Cheng

2011 ◽  
Vol 27 (3) ◽  
pp. 733-740
Author(s):  
V. Gerzilov ◽  
P. Rashev ◽  
A. Bochukov ◽  
P. Bonchev

A study for the influence of semen extenders IMV-buffer, HIA- 1 and AU on sperm motion characteristics of Muscovy drake spermatozoa was carried out. The semen from each male (n=6) was divided into three equal parts and diluted in ratio 1:3 (semen:extender) with the IMV-buffer, HIA-1 and AU respectively, and then was stored at temperature 0-4C? for 6 hours. Sperm motion parameters - velocity of spermatozoa (rapid, medium, slow and statistic), VCL, VSL, VAP, LIN, STR, WOB were measured using a Sperm Class Analizer (Micropticum, Spain). Computer-assisted sperm motion analysis indicated that Muscovy spermatozoa preserved a rapid and medium sperm velocity after 6 hours in vitro storage in three examination extenders. The total VCL, VSL and VAP of spermatozoa in the semen diluted with AU extender were 110.47?7.44 ?m/s, 29.42?2.02 ?m/s and 57.39?3.73 ?m/s, in the semen diluted with IMV-buffer were 94.93?11.10 ?m/s, 27.57?2.45 ?m/s and 51.35?4.98 ?m/s, and in the semen diluted with HIA-1 were 68.48?12.74 ?m/s, 20.08?4.18 ?m/s and 37.75?7.65 ?m/s, as the differences were significant between AU and HIA-1 - (P<0.05). About LIN, STR, WOB there were no significant differences for the influence of the extenders.


2006 ◽  
Vol 39 (4) ◽  
pp. 259-268 ◽  
Author(s):  
Margaret O. Amsler ◽  
Charles D. Amsler ◽  
Dan Rittschof ◽  
Mikel A. Becerro ◽  
James B. Mcclintock

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