Analysis of Monoclonal Antibody Chimeric BR96-Doxorubicin Immunoconjugate by Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis with Ultraviolet and Laser-Induced Fluorescence Detection

1995 ◽  
Vol 229 (2) ◽  
pp. 221-228 ◽  
Author(s):  
J.P. Liu ◽  
S. Abid ◽  
M.S. Lee
Keyword(s):  
Monoclonal Antibody ◽  
Sodium Dodecyl Sulfate ◽  
Fluorescence Detection ◽  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Laser Induced Fluorescence ◽  
Capillary Gel Electrophoresis ◽  
Laser Induced Fluorescence Detection ◽  
Dodecyl Sulfate

Quantification of green fluorescent protein-(GFP-) tagged membrane proteins by capillary gel electrophoresis

The Analyst ◽  
2017 ◽  
Vol 142 (19) ◽  
pp. 3648-3655
Author(s):  
Azeem Danish ◽  
Sang-Yong Lee ◽  
Christa E. Müller
Keyword(s):  
Green Fluorescent Protein ◽  
Membrane Proteins ◽  
Fluorescence Detection ◽  
Gel Electrophoresis ◽  
Fluorescent Protein ◽  
Laser Induced Fluorescence ◽  
Capillary Gel Electrophoresis ◽  
Laser Induced Fluorescence Detection ◽  
Green Fluorescent

A fast and robust procedure for the quantification of GFP-tagged membrane proteins in cell homogenates was developed employing capillary gel electrophoresis coupled to laser-induced fluorescence detection (CGE-LIF).

Quantification of Whey Protein Content in Infant Formulas by Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis (SDS-CGE): Single-Laboratory Validation, First Action 2016.15

2017 ◽  
Vol 100 (2) ◽  
pp. 510-521 ◽  
Author(s):  
Ping Feng ◽  
Christophe Fuerer ◽  
Adrienne McMahon
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Protein Content ◽  
Whey Protein ◽  
Gel Electrophoresis ◽  
Whey Proteins ◽  
Sodium Dodecyl ◽  
Infant Formulas ◽  
Method Performance ◽  
Capillary Gel Electrophoresis ◽  
Dodecyl Sulfate

Abstract Protein separation by sodium dodecyl sulfate-capillary gel electrophoresis, followed by UV absorption at 220 nm, allows for the quantification of major proteins in raw milk. In processed dairy samples such as skim milk powder (SMP) and infant formulas, signals from individual proteins are less resolved, but caseins still migrate as one family between two groups of whey proteins. In the first group, α-lactalbumin and β-lactoglobulin migrate as two distinct peaks. Lactosylated adducts show delayed migration times and interfere with peak separation, but both native and modified forms as well as other low-MW whey proteins still elute before the caseins. The second group contains high-MW whey proteins (including bovine serum albumin, lactoferrin, and immunoglobulins) and elutes after the caseins. Caseins and whey proteins can thus be considered two distinct nonoverlapping families whose ratio can be established based on integrated areas without the need for a calibration curve. Because mass-to-area response factors for whey proteins and caseins are different, an area correction factor was determined from experimental measurement using SMP. Method performance assessed on five infant formulas showed RSDs of 0.2–1.2% (within day) and 0.5–1.1% (multiple days), with average recoveries between 97.4 and 106.4% of added whey protein. Forty-three different infant formulas and milk powders were analyzed. Of the 41 samples with manufacturer claims, the measured whey protein content was in close agreement with declared values, falling within 5% of the declared value in 76% of samples and within 10% in 95% of samples.

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