Primary Structure Characterization of a Rhodocyclus tenuis Diheme Cytochrome c Reveals the Existence of Two Different Classes of Low-Potential Diheme Cytochromes c in Purple Phototropic Bacteria

2000 ◽  
Vol 381 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Bart Devreese ◽  
Ann Brigé ◽  
Katrien Backers ◽  
Gonzalez Van Driessche ◽  
Terrance E Meyer ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Primary Structure ◽  
Structure Characterization ◽  
Cytochromes C ◽  
Rhodocyclus Tenuis

scholarly journals Primary Structure Characterization of the Photosystem II D1 and D2 Subunits

1997 ◽  
Vol 272 (52) ◽  
pp. 33158-33166 ◽  
Author(s):  
Jyoti Sharma ◽  
Maria Panico ◽  
Catherine A. Shipton ◽  
Fredrick Nilsson ◽  
Howard R. Morris ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Primary Structure ◽  
Structure Characterization

Murine Ortholog of the Novel Glycosyltransferase, B3GTL: Primary Structure, Characterization of the Gene and Transcripts, and Expression in Tissues

2006 ◽  
Vol 25 (8) ◽  
pp. 465-474 ◽  
Author(s):  
Taisto Y.K. Heinonen ◽  
Markku Pelto-Huikko ◽  
Leena Pasternack ◽  
Markku Mäki ◽  
Heikki Kainulainen
Keyword(s):  
Primary Structure ◽  
Structure Characterization ◽  
The Novel

scholarly journals A DELETION MAP OF cyc1 MUTANTS AND ITS CORRESPONDENCE TO MUTATIONALLY ALTERED ISO-1-CYTOCHROMES c OF YEAST

Genetics ◽  
1975 ◽  
Vol 81 (1) ◽  
pp. 51-73 ◽  
Author(s):  
Fred Sherman ◽  
Mary Jackson ◽  
Susan W Liebman ◽  
Ann Marie Schweingruber ◽  
John W Stewart
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Cytochrome C ◽  
Primary Structure ◽  
Recombination Rates ◽  
Yeast Saccharomyces Cerevisiae ◽  
X Ray ◽  
Cytochromes C ◽  
Adjacent Point

ABSTRACT Mutants arising spontaneously from sporulated cultures of certain strains of yeast, Saccharomyces cerevisiae, contained deletions of the CYC1 gene which controls the primary structure of iso-1-cytochrome c. At least 60 different kinds of deletions were uncovered among the 104 deletions examined and these ranged in length from those encompassing only two adjacent point mutants to those encompassing at least the entire CYC1 gene. X-ray-induced recombination rates of crosses involving these deletions and cyc1 point mutants resulted in the assignment of 211 point mutants to 47 mutational sites and made it possible to unambiguously order 40 of these 47 sites. Except for one mutant, cyc1-15, there was a strict colinear relationship between the deletion map and the positions of 13 sites that were previously determined by amino acid alterations in iso-1-cytochromes c from intragenic revertants.

Characterization of CDR3 Structure of Vβ21 T Cell Clones In Patients with P210BCR-ABL Positive CML and B-ALL

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 4455-4455
Author(s):  
Xianfeng Zha ◽  
Shaohua Chen ◽  
Lijian Yang ◽  
Bo Li ◽  
Yu Chen ◽  
...  
Keyword(s):  
Amino Acid ◽  
Primary Structure ◽  
Three Dimensional ◽  
Structure Characterization ◽  
Sequencing Analysis ◽  
Amino Acid Motif ◽  
Cdr3 Region ◽  
Cell Clones ◽  
Tumor Associated Antigen

Abstract Abstract 4455 The clonally expanded T cells identified in most cancer patients which were considered to respond to tumour association antigen (TAA) have definitely specific anti-tumor cytotoxicity. P210BCR-ABL protein regards as the hallmark of CML and known to be causative of the disease, and also expresses in partial acute lymphoblastic leukaemia (ALL) can induce to yield tumor-specific cytotoxic T lymphocytes (CTL) which can kill bar-abl+ tumor cells in many trials. In our previous study, we found the oligoclonal expansion of TCR Vβ21 subfamily in the peripheral blood (PB) of 8 cases with P210BCR-ABL positive CML and 3 cases with P210BCR-ABL positive B-ALL patients and 1 case with P210BCR-ABL negative B-ALL patients using the genescan technology. To further elucidate the oligoclonal TCR Vβ21 features, we carried out sequencing analysis of CDR3 region of each oligoclonal TCR Vβ21 and determined the primary structure of the CDR3 region. The 12 TCR Vβ21 clones used different Jβ segments, Jβ2.3 segment was used in 4 clones, both Jβ2.1 and Jβ1.1 segments were used in 2 clones respectively, Jβ1.2, Jβ1.3, Jβ2.2 and Jβ2.7 segments were used in 1 clone. Only a conserved amino acid motif (SLxxV) was found within the CDR3 region from 3 patients with CML. Then we constructed the three-dimensional structures of the CDR3 sequences from all 12 clones by homology modeling methods and through pairwise comparing with the three-dimensional structures of all CDR3 sequences, the results showed that the conformation of the CDR3 region either containing the conserved amino acid motif or using the same Jβ segment displayed low similarity. Interesting, the conformation among some clones whose CDR3 region contain different amino acid motif and did not use the same Jβ segment were high resemble. In conclusions, our findings described that the high frequency of TCR Vβ21 subfamily expansion emerged in p210BCR-ABL positive CML and B-ALL patients, and according to perplexing features of CDR3 region, it could not easy to confirmed that the oligoclonal expanded TCR Vβ21 subfamily must recognize to p210BCR-ABL protein, however, primary structure characterization of CDR3 region, in combination with spatial structure features, ultimately might help to identify the tumor-associated antigen immune attack on tumor cell in p210BCR-ABL positive CML or B-ALL patients. Disclosures: Li: The study was supported by grants from National “863” project (No. 2006AA02Z114) and Key project of Natural Science Foundation of Guangdong province, China (No. 05103293, 9251063201000001).: Research Funding.

scholarly journals The primary structure of cytochrome c from the rust fungus Ustilago sphaerogena

1972 ◽  
Vol 129 (3) ◽  
pp. 561-569 ◽  
Author(s):  
K. G. Bitar ◽  
S. N. Vinogradov ◽  
C. Nolan ◽  
L. J. Weiss ◽  
E. Margoliash
Keyword(s):  
Amino Acid ◽  
Cytochrome C ◽  
Primary Structure ◽  
Polypeptide Chain ◽  
Rust Fungus ◽  
Single Polypeptide Chain ◽  
C Terminus ◽  
Cytochromes C ◽  
Single Polypeptide ◽  
Asparagine Residue

1. The complete amino acid sequence of cytochrome c from the basidiomycete Ustilago sphaerogena was determined from the amino acid compositions and sequences of either tryptic or chymotryptic peptides, and in homology with at least thirty other established sequences of cytochrome c. 2. The primary structure of the molecule bears all of the characteristics of a mammalian-type cytochrome c, showing the typical clustered distribution of hydrophobic and basic residues with a single polypeptide chain of 107 residues. 3. Like all other fungal cytochromes c, it possesses a free N-terminus, and one less residue at the C-terminus than vertebrate cytochromes c. The region of residues 70–80 is strictly conserved, as is histidine at position 18. Position 26 is occupied by an asparagine residue, in contrast to histidine which occurs at this location in most of the known sequences of mammalian-type cytochromes c. 4. In contrast to some other fungal and plant cytochromes c of known primary structures, the Ustilago cytochrome c molecule does not contain trimethyl-lysine. 5. The sequence of Ustilago cytochrome c differs from the sequences of human, horse, chicken, tuna, wheat, and baker's yeast proteins at loci 47, 43, 44, 44 and 38 respectively.

scholarly journals Metal-Free and Metal-Substituted Cytochromes c. Use in Characterization of the Cytochrome c Binding Site

1977 ◽  
Vol 81 (2) ◽  
pp. 339-347 ◽  
Author(s):  
Jane M. VANDERKOOI ◽  
Regina LANDESBERG ◽  
Geoffrey W. HAYDEN ◽  
Charles S. OWEN
Keyword(s):  
Cytochrome C ◽  
Binding Site ◽  
Metal Free ◽  
Cytochromes C

Characterization of microwave-sintered ceramic composites

1993 ◽  
Vol 51 ◽  
pp. 1136-1137
Author(s):  
X. Zhang ◽  
Y. Pan ◽  
T.T. Meek
Keyword(s):  
Matrix Material ◽  
Maximum Output Power ◽  
Ceramic Matrix Composite ◽  
Ceramic Composites ◽  
Processing Technique ◽  
Structure Characterization ◽  
Alumina Powder ◽  
Maximum Output ◽  
Sintered Ceramic

Industrial microwave heating technology has emerged as a new ceramic processing technique. The unique advantages of fast sintering, high density, and improved materials properties makes it superior in certain respects to other processing methods. This work presents the structure characterization of a microwave sintered ceramic matrix composite.Commercial α-alumina powder A-16 (Alcoa) is chosen as the matrix material, β-silicon carbide whiskers (Third Millennium Technologies, Inc.) are used as the reinforcing element. The green samples consisted of 90 vol% Al2O3 powder and 10 vol% ultrasonically-dispersed SiC whiskers. The powder mixture is blended together, and then uniaxially pressed into a cylindrical pellet under a pressure of 230 MPa, which yields a 52% green density. The sintering experiments are carried out using an industry microwave system (Gober, Model S6F) which generates microwave radiation at 2.45 GHz with a maximum output power of 6 kW. The composites are sintered at two different temperatures (1550°C and 1650°C) with various isothermal processing time intervals ranging from 10 to 20 min.

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