Coexpression of Genetically Engineered Fused Enzyme between Yeast NADPH–P450 Reductase and Human Cytochrome P450 3A4 and Human Cytochrome b5 in Yeast

Koji Hayashi; Toshiyuki Sakaki; Shiro Kominami; Kuniyo Inouye; Yoshiyasu Yabusaki

doi:10.1006/abbi.2000.1953

Coexpression of Genetically Engineered Fused Enzyme between Yeast NADPH–P450 Reductase and Human Cytochrome P450 3A4 and Human Cytochrome b5 in Yeast

Archives of Biochemistry and Biophysics ◽

10.1006/abbi.2000.1953 ◽

2000 ◽

Vol 381 (1) ◽

pp. 164-170 ◽

Cited By ~ 22

Author(s):

Koji Hayashi ◽

Toshiyuki Sakaki ◽

Shiro Kominami ◽

Kuniyo Inouye ◽

Yoshiyasu Yabusaki

Keyword(s):

Cytochrome P450 ◽

Cytochrome B5 ◽

Genetically Engineered ◽

Cytochrome P450 3A4 ◽

P450 Reductase ◽

Human Cytochrome

Download Full-text

Co-expression of human cytochrome b5 increases expression of cytochrome P450 3A4 in Escherichia coli by stabilizing mRNA

Protein Expression and Purification ◽

10.1016/j.pep.2013.02.010 ◽

2013 ◽

Vol 89 (1) ◽

pp. 44-50 ◽

Cited By ~ 5

Author(s):

Mi-Sook Dong ◽

Sang-Bum Lee ◽

Hyun-Jung Kim

Keyword(s):

Escherichia Coli ◽

Cytochrome P450 ◽

Cytochrome B5 ◽

Cytochrome P450 3A4 ◽

Human Cytochrome

Download Full-text

Role of human cytochrome P450 (CYP) in the metabolic activation of N-alkylnitrosamines: application of genetically engineered Salmonella typhimurium YG7108 expressing each form of CYP together with human NADPH-cytochrome P450 reductase

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis ◽

10.1016/s0027-5107(01)00223-8 ◽

2001 ◽

Vol 483 (1-2) ◽

pp. 35-41 ◽

Cited By ~ 40

Author(s):

Ken-ichi Fujita ◽

Tetsuya Kamataki

Keyword(s):

Cytochrome P450 ◽

Salmonella Typhimurium ◽

Metabolic Activation ◽

Genetically Engineered ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

Download Full-text

Human cytochrome P450 3A4: enzymatic properties of a purified recombinant fusion protein containing NADPH-P450 reductase.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.90.24.11748 ◽

1993 ◽

Vol 90 (24) ◽

pp. 11748-11752 ◽

Cited By ~ 84

Author(s):

M. S. Shet ◽

C. W. Fisher ◽

P. L. Holmans ◽

R. W. Estabrook

Keyword(s):

Cytochrome P450 ◽

Fusion Protein ◽

Enzymatic Properties ◽

Recombinant Fusion Protein ◽

Cytochrome P450 3A4 ◽

P450 Reductase ◽

Human Cytochrome

Download Full-text

Biphasic effects of the flavonoids quercetin and naringenin on the metabolic activation of 2-amino-3,5-dimethylimidazo[4,5-f]quinoline by Salmonella typhimurium TA1538 co-expressing human cytochrome P450 1A2, NADPH-cytochrome P450 reductase, and cytochrome b5

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis ◽

10.1016/j.mrfmmm.2003.08.002 ◽

2004 ◽

Vol 545 (1-2) ◽

pp. 37-47 ◽

Cited By ~ 21

Author(s):

I Kang

Keyword(s):

Cytochrome P450 ◽

Salmonella Typhimurium ◽

Cytochrome B5 ◽

Metabolic Activation ◽

Cytochrome P450 Reductase ◽

Cytochrome P450 1A2 ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

Download Full-text

Reaction of Human Cytochrome P450 3A4 with Peroxynitrite: Nitrotyrosine Formation on the Proximal Side Impairs Its Interaction with NADPH-Cytochrome P450 Reductase

Chemical Research in Toxicology ◽

10.1021/tx3002753 ◽

2012 ◽

Vol 25 (12) ◽

pp. 2642-2653 ◽

Cited By ~ 19

Author(s):

Hsia-lien Lin ◽

Cesar Kenaan ◽

Haoming Zhang ◽

Paul F. Hollenberg

Keyword(s):

Cytochrome P450 ◽

Cytochrome P450 3A4 ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

Proximal Side ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

Download Full-text

Genetically Engineered Bacterial Cells Co-expressing Human Cytochrome P450 with NADPH-cytochrome P450 Reductase: Prediction of Metabolism and Toxicity of Drugs in Humans

Drug Metabolism and Pharmacokinetics ◽

10.2133/dmpk.17.1 ◽

2002 ◽

Vol 17 (1) ◽

pp. 1-22 ◽

Cited By ~ 14

Author(s):

Ken-ichi Fujita ◽

Tetsuya Kamataki

Keyword(s):

Cytochrome P450 ◽

Genetically Engineered ◽

Bacterial Cells ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase ◽

Toxicity Of Drugs

Download Full-text

Establishment of ten strains of genetically engineered Salmonella typhimurium TA1538 each co-expressing a form of human cytochrome P450 with NADPH-cytochrome P450 reductase sensitive to various promutagens

Mutation Research/Genetic Toxicology and Environmental Mutagenesis ◽

10.1016/j.mrgentox.2004.06.003 ◽

2004 ◽

Vol 562 (1-2) ◽

pp. 151-162 ◽

Cited By ~ 18

Author(s):

Yoshiyuki Yamazaki ◽

Ken-ichi Fujita ◽

Kazuo Nakayama ◽

Akihiro Suzuki ◽

Katsunori Nakamura ◽

...

Keyword(s):

Cytochrome P450 ◽

Salmonella Typhimurium ◽

Genetically Engineered ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

Download Full-text

Optimization of yeast-expressed human liver cytochrome P450 3A4 catalytic activities by coexpressing NADPH-cytochrome P450 reductase and cytochrome b5

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1992.tb17027.x ◽

1992 ◽

Vol 207 (1) ◽

pp. 109-116 ◽

Cited By ~ 62

Author(s):

Marie-Anne PEYRONNEAU ◽

Jean-Paul RENAUD ◽

Gilles TRUAN ◽

Philippe URBAN ◽

Denis POMPON ◽

...

Keyword(s):

Cytochrome P450 ◽

Human Liver ◽

Cytochrome B5 ◽

Cytochrome P450 3A4 ◽

Cytochrome P450 Reductase ◽

Catalytic Activities ◽

Liver Cytochrome ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Nadph Cytochrome P450 Reductase

Download Full-text

CO Binding Kinetics of Human Cytochrome P450 3A4

Journal of Biological Chemistry ◽

10.1074/jbc.270.10.5014 ◽

1995 ◽

Vol 270 (10) ◽

pp. 5014-5018 ◽

Cited By ~ 56

Author(s):

Aditya P. Koley ◽

Jeroen T. M. Buters ◽

Richard C. Robinson ◽

Allen Markowitz ◽

Fred K. Friedman

Keyword(s):

Cytochrome P450 ◽

Binding Kinetics ◽

Cytochrome P450 3A4 ◽

Human Cytochrome ◽

Kinetics Of

Download Full-text

NADH:cytochrome b5 reductase and cytochrome b5 act as sole electron donors to human cytochrome P450 1A1-catalyzed oxidation and DNA adduct formation by benzo[a]pyrene

Toxicology Letters ◽

10.1016/j.toxlet.2016.06.1417 ◽

2016 ◽

Vol 258 ◽

pp. S96

Author(s):

M. Stiborova ◽

I. Radek ◽

M. Moserova ◽

E. Frei ◽

K. Kopka ◽

...

Keyword(s):

Cytochrome P450 ◽

Cytochrome B5 ◽

Adduct Formation ◽

Dna Adduct ◽

Electron Donors ◽

Cytochrome P450 1A1 ◽

Human Cytochrome ◽

Catalyzed Oxidation

Download Full-text