Genetically Engineered Bacterial Cells Co-expressing Human Cytochrome P450 with NADPH-cytochrome P450 Reductase: Prediction of Metabolism and Toxicity of Drugs in Humans

Ken-ichi Fujita; Tetsuya Kamataki

doi:10.2133/dmpk.17.1

Genetically Engineered Bacterial Cells Co-expressing Human Cytochrome P450 with NADPH-cytochrome P450 Reductase: Prediction of Metabolism and Toxicity of Drugs in Humans

Drug Metabolism and Pharmacokinetics ◽

10.2133/dmpk.17.1 ◽

2002 ◽

Vol 17 (1) ◽

pp. 1-22 ◽

Cited By ~ 14

Author(s):

Ken-ichi Fujita ◽

Tetsuya Kamataki

Keyword(s):

Cytochrome P450 ◽

Genetically Engineered ◽

Bacterial Cells ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase ◽

Toxicity Of Drugs

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Role of human cytochrome P450 (CYP) in the metabolic activation of N-alkylnitrosamines: application of genetically engineered Salmonella typhimurium YG7108 expressing each form of CYP together with human NADPH-cytochrome P450 reductase

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis ◽

10.1016/s0027-5107(01)00223-8 ◽

2001 ◽

Vol 483 (1-2) ◽

pp. 35-41 ◽

Cited By ~ 40

Author(s):

Ken-ichi Fujita ◽

Tetsuya Kamataki

Keyword(s):

Cytochrome P450 ◽

Salmonella Typhimurium ◽

Metabolic Activation ◽

Genetically Engineered ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

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Establishment of ten strains of genetically engineered Salmonella typhimurium TA1538 each co-expressing a form of human cytochrome P450 with NADPH-cytochrome P450 reductase sensitive to various promutagens

Mutation Research/Genetic Toxicology and Environmental Mutagenesis ◽

10.1016/j.mrgentox.2004.06.003 ◽

2004 ◽

Vol 562 (1-2) ◽

pp. 151-162 ◽

Cited By ~ 18

Author(s):

Yoshiyuki Yamazaki ◽

Ken-ichi Fujita ◽

Kazuo Nakayama ◽

Akihiro Suzuki ◽

Katsunori Nakamura ◽

...

Keyword(s):

Cytochrome P450 ◽

Salmonella Typhimurium ◽

Genetically Engineered ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

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Construction ofSalmonella typhimurium YG7108 strains, each coexpressing a form of human cytochrome P450 with NADPH-cytochrome P450 reductase

Environmental and Molecular Mutagenesis ◽

10.1002/em.10034 ◽

2001 ◽

Vol 38 (4) ◽

pp. 329-338 ◽

Cited By ~ 14

Author(s):

Ken-ichi Fujita ◽

Kazuo Nakayama ◽

Yoshiyuki Yamazaki ◽

Kazuhiro Tsuruma ◽

Masami Yamada ◽

...

Keyword(s):

Cytochrome P450 ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

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Drug-Drug Interactions Evaluated by a Highly Active Reconstituted Native Human Cytochrome P4503A4 and Human NADPH-Cytochrome P450 Reductase System

Arzneimittelforschung ◽

10.1055/s-0031-1296940 ◽

2011 ◽

Vol 54 (01) ◽

pp. 78-83

Author(s):

Thomas Haehner ◽

Mohamed Refaie ◽

Dieter Müller-Enoch

Keyword(s):

Cytochrome P450 ◽

Drug Interactions ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Highly Active ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

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Use of genetically engineeredSalmonella typhimurium OY1002/1A2 strain coexpressing human cytochrome P450 1A2 and NADPH-cytochrome P450 reductase and bacterialO-acetyltransferase in SOS/umu assay

Environmental and Molecular Mutagenesis ◽

10.1002/1098-2280(2000)36:2<121::aid-em6>3.0.co;2-p ◽

2000 ◽

Vol 36 (2) ◽

pp. 121-126 ◽

Cited By ~ 16

Author(s):

Pramod Aryal ◽

Takao Terashita ◽

F. Peter Guengerich ◽

Tsutomu Shimada ◽

Yoshimitsu Oda

Keyword(s):

Cytochrome P450 ◽

Cytochrome P450 Reductase ◽

Cytochrome P450 1A2 ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

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Mutagenic activation of betel quid-specific N-nitrosamines catalyzed by human cytochrome P450 coexpressed with NADPH-cytochrome P450 reductase in Salmonella typhimurium YG7108

Mutation Research/Genetic Toxicology and Environmental Mutagenesis ◽

10.1016/j.mrgentox.2004.12.002 ◽

2005 ◽

Vol 581 (1-2) ◽

pp. 165-171 ◽

Cited By ~ 20

Author(s):

Masafumi Miyazaki ◽

Eika Sugawara ◽

Teruki Yoshimura ◽

Hiroshi Yamazaki ◽

Tetsuya Kamataki

Keyword(s):

Cytochrome P450 ◽

Salmonella Typhimurium ◽

Betel Quid ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

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Biphasic effects of the flavonoids quercetin and naringenin on the metabolic activation of 2-amino-3,5-dimethylimidazo[4,5-f]quinoline by Salmonella typhimurium TA1538 co-expressing human cytochrome P450 1A2, NADPH-cytochrome P450 reductase, and cytochrome b5

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis ◽

10.1016/j.mrfmmm.2003.08.002 ◽

2004 ◽

Vol 545 (1-2) ◽

pp. 37-47 ◽

Cited By ~ 21

Author(s):

I Kang

Keyword(s):

Cytochrome P450 ◽

Salmonella Typhimurium ◽

Cytochrome B5 ◽

Metabolic Activation ◽

Cytochrome P450 Reductase ◽

Cytochrome P450 1A2 ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

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Metabolic activation of N -alkylnitrosamines in genetically engineered Salmonella typhimurium expressing CYP2E1 or CYP2A6 together with human NADPH-cytochrome P450 reductase

Carcinogenesis ◽

10.1093/carcin/21.6.1227 ◽

2000 ◽

Vol 21 (6) ◽

pp. 1227-1232 ◽

Cited By ~ 39

Author(s):

Hirotaka Kushida ◽

Ken-ichi Fujita ◽

Akihiro Suzuki ◽

Masami Yamada ◽

Toru Endo ◽

...

Keyword(s):

Cytochrome P450 ◽

Salmonella Typhimurium ◽

Metabolic Activation ◽

Genetically Engineered ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Nadph Cytochrome P450 Reductase

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Recombinant Human Cytochrome P450 1A2 and an N-Terminal-Truncated Form: Construction, Purification, Aggregation Properties, and Interactions with Flavodoxin, Ferredoxin, and NADPH-Cytochrome P450 Reductase

Archives of Biochemistry and Biophysics ◽

10.1006/abbi.1996.0086 ◽

1996 ◽

Vol 327 (1) ◽

pp. 11-19 ◽

Cited By ~ 46

Author(s):

Mi-Sook Dong ◽

Hiroshi Yamazaki ◽

Zuyu Guo ◽

F.Peter Guengerich

Keyword(s):

Cytochrome P450 ◽

Cytochrome P450 Reductase ◽

Truncated Form ◽

Cytochrome P450 1A2 ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

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Escherichia coli MTC, a NADPH cytochrome P450 reductase competent mutagenicity tester strain for the expression of human cytochrome P450: Comparison of three types of expression systems

Mutation Research/Genetic Toxicology and Environmental Mutagenesis ◽

10.1016/s1383-5718(98)00193-4 ◽

1999 ◽

Vol 439 (2) ◽

pp. 287-300 ◽

Cited By ~ 19

Author(s):

Michel Kranendonk ◽

Charles W Fisher ◽

Rita Roda ◽

Filipa Carreira ◽

Patricia Theisen ◽

...

Keyword(s):

Escherichia Coli ◽

Cytochrome P450 ◽

Cytochrome P450 Reductase ◽

Expression Systems ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Human Cytochrome ◽

Nadph Cytochrome P450 Reductase

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