Metallothionein-IIAPromoter Induction Alters Rat Intestinal Fatty Acid Binding Protein Expression, Fatty Acid Uptake, and Lipid Metabolism in Transfected L-Cells

1997 ◽  
Vol 340 (1) ◽  
pp. 135-143 ◽  
Author(s):  
Daniel R. Prows ◽  
Friedhelm Schroeder
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Protein Expression ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Uptake ◽  
Acid Uptake ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
L Cells

scholarly journals Transfection of L6 myoblasts with adipocyte fatty acid-binding protein cDNA does not affect fatty acid uptake but disturbs lipid metabolism and fusion

1998 ◽  
Vol 329 (2) ◽  
pp. 265-273 ◽  
Author(s):  
F. M. Clemens PRINSEN ◽  
H. Jacques VEERKAMP
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Metabolism ◽  
Fatty Acid Binding Protein ◽  
Acid Metabolism ◽  
Binding Protein ◽  
Fatty Acid Uptake ◽  
Acid Uptake ◽  
Fatty Acid Binding ◽  
Transfected Cells ◽  
Acid Binding Protein

We studied the involvement of fatty acid-binding protein (FABP) in growth, differentiation and fatty acid metabolism of muscle cells by lipofection of rat L6 myoblasts with rat heart (H) FABP cDNA or with rat adipocyte (A) FABP cDNA in a eukaryotic expression vector which contained a puromycin acetyltransferase cassette. Stable transfectants showed integration into the genome for all constructs and type-specific overexpression at the mRNA and protein level for the clones with H-FABP and A-FABP cDNA constructs. The rate of proliferation of myoblasts transfected with rat A-FABP cDNA was 2-fold higher compared with all other transfected cells. In addition, these myoblasts showed disturbed fusion and differentiation, as assessed by morphological examination and creatine kinase activity. Uptake rates of palmitate were equal for all clone types, in spite of different FABP content and composition. Palmitate oxidation over a 3 h period was similar in all clones from growth medium. After being cultured in differentiation medium, mock- and H-FABP-cDNA-transfected cells showed a lower fatty acid-oxidation rate, in contrast with A-FABP-cDNA-transfected clones. The ratio of [14C]palmitic acid incorporation into phosphatidylcholine and phosphatidylethanolamine of A-FABP-cDNA-transfected clones changed in the opposite direction in differentiation medium from that of mock- and H-FABP-cDNA-transfected clones. In conclusion, transfection of L6 myoblasts with A-FABP cDNA does not affect H-FABP content and fatty acid uptake, but changes fatty acid metabolism. The latter changes may be related to the observed fusion defect.

scholarly journals Heart Fatty Acid Uptake Is Decreased in Heart Fatty Acid-binding Protein Gene-ablated Mice

2004 ◽  
Vol 279 (33) ◽  
pp. 34481-34488 ◽  
Author(s):  
Eric J. Murphy ◽  
Gwendolyn Barcelo-Coblijn ◽  
Bert Binas ◽  
Jan F. C. Glatz
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Protein Gene ◽  
Binding Protein ◽  
Fatty Acid Uptake ◽  
Acid Uptake ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Binding Protein Gene

Dietary fiber differentially alters cellular fatty acid‐binding protein expression in exfoliated colonocytes during tumor development

1998 ◽  
Vol 32 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Robert S. Chapkin ◽  
Amy E. Clark ◽  
Laurie A. Davidson ◽  
Friedhelm Schroeder ◽  
Debra L. Zoran ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Protein Expression ◽  
Dietary Fiber ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Tumor Development ◽  
Cellular Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein
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