Expression Cloning of a Novel Farnesylated Protein, RDJ2, Encoding a DnaJ Protein Homologue

1997 ◽  
Vol 346 (1) ◽  
pp. 113-124 ◽  
Author(s):  
Douglas A. Andres ◽  
Haipeng Shao ◽  
Dean C. Crick ◽  
Brian S. Finlin
Keyword(s):  
Expression Cloning ◽  
Dnaj Protein ◽  
Farnesylated Protein

scholarly journals Expression Cloning of Mouse cDNA of CMP-NeuAc:Lactosylceramide α2,3-Sialyltransferase, an Enzyme That Initiates the Synthesis of Gangliosides

1999 ◽  
Vol 274 (14) ◽  
pp. 9271-9276 ◽  
Author(s):  
Satoshi Fukumoto ◽  
Hiroshi Miyazaki ◽  
George Goto ◽  
Takeshi Urano ◽  
Keiko Furukawa ◽  
...  
Keyword(s):  
Expression Cloning ◽  
Mouse Cdna

scholarly journals A role for CD9 molecules in T cell activation.

1996 ◽  
Vol 184 (2) ◽  
pp. 753-758 ◽  
Author(s):  
X G Tai ◽  
Y Yashiro ◽  
R Abe ◽  
K Toyooka ◽  
C R Wood ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Immune Responses ◽  
T Cell Activation ◽  
Cell Activation ◽  
Expression Cloning ◽  
Wild Type ◽  
Almost All ◽  
Antigen Presenting ◽  
Deficient Mice

Costimulation mediated by the CD28 molecule plays an important role in optimal activation of T cells. However, CD28-deficient mice can mount effective T cell-dependent immune responses, suggesting the existence of other costimulatory systems. In a search for other costimulatory molecules on T cells, we have developed a monoclonal antibody (mAb) that can costimulate T cells in the absence of antigen-presenting cells (APC). The molecule recognized by this mAb, 9D3, was found to be expressed on almost all mature T cells and to be a protein of approximately 24 kD molecular mass. By expression cloning, this molecule was identified as CD9, 9D3 (anti-CD9) synergized with suboptimal doses of anti-CD3 mAb in inducing proliferation by virgin T cells. Costimulation was induced by independent ligation of CD3 and CD9, suggesting that colocalization of these two molecules is not required for T cell activation. The costimulation by anti-CD9 was as potent as that by anti-CD28. Moreover, anti-CD9 costimulated in a CD28-independent way because anti-CD9 equally costimulated T cells from the CD28-deficient as well as wild-type mice. Thus, these results indicate that CD9 serves as a molecule on T cells that can deliver a potent CD28-independent costimulatory signal.

scholarly journals Identification of a CD4+ T cell-stimulating antigen of pathogenic bacteria by expression cloning.

1995 ◽  
Vol 182 (6) ◽  
pp. 1751-1757 ◽  
Author(s):  
S Sanderson ◽  
D J Campbell ◽  
N Shastri
Keyword(s):  
T Cells ◽  
Listeria Monocytogenes ◽  
T Cell ◽  
Mhc Class Ii ◽  
T Cell Activation ◽  
Pathogenic Bacteria ◽  
Genomic Library ◽  
Class Ii ◽  
Cd4 T Cell ◽  
Expression Cloning

Identifying the immunogenic proteins that elicit pathogen-specific T cell responses is key to rational vaccine design. While several approaches have succeeded in identifying major histocompatibility complex (MHC) class I bound peptides that stimulate CD8+ T cells, these approaches have been difficult to extend to peptides presented by MHC class II molecules that stimulate CD4+ T cells. We describe here a novel strategy for identifying CD4+ T cell-stimulating antigen genes. Using Listeria monocytogenes-specific, lacZ-inducible T cells as single-cell probes, we screened a Listeria monocytogenes genomic library as recombinant Escherichia coli that were fed to macrophages. The antigen gene was isolated from the E. coli clone that, when ingested by the macrophages, allowed generation of the appropriate peptide/MHC class II complex and T cell activation. We show that the antigenic peptide is derived from a previously unknown listeria gene product with characteristics of a membrane-bound protein.

scholarly journals Expression cloning of a cDNA encoding a fish prolactin receptor.

1995 ◽  
Vol 92 (13) ◽  
pp. 6037-6041 ◽  
Author(s):  
O. Sandra ◽  
F. Sohm ◽  
A. de Luze ◽  
P. Prunet ◽  
M. Edery ◽  
...  
Keyword(s):  
Prolactin Receptor ◽  
Expression Cloning

Expression cloning of a diphtheria toxin receptor: Identity with a heparin-binding EGF-like growth factor precursor

Cell ◽  
1992 ◽  
Vol 69 (6) ◽  
pp. 1051-1061 ◽  
Author(s):  
Joseph G. Naglich ◽  
James E. Metherall ◽  
David W. Russell ◽  
Leon Eidels
Keyword(s):  
Growth Factor ◽  
Diphtheria Toxin ◽  
Expression Cloning ◽  
Heparin Binding ◽  
Diphtheria Toxin Receptor ◽  
Toxin Receptor
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