Free Radicals Induce Reversible Membrane-Cytoplasm Translocation of Glyceraldehyde-3-Phosphate Dehydrogenase in Human Erythrocytes

1995 ◽  
Vol 321 (2) ◽  
pp. 345-352 ◽  
Author(s):  
C. Mallozzi ◽  
A.M.M. Distasi ◽  
M. Minetti
Keyword(s):  
Free Radicals ◽  
Human Erythrocytes

Zawartość związków fenolowych w ekstrakcie z propolisu oraz ocena jego aktywności przeciwutleniającej i cytoochronnej względem erytrocytów ludzkich w warunkach stresu oksydacyjnego in vitro

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Agnieszka Waśkiewicz ◽  
Marta Babicka ◽  
Elżbieta Hołderna-Kędzia ◽  
...  
Keyword(s):  
Free Radicals ◽  
Phenolic Acids ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Human Erythrocytes ◽  
Antioxidant Potential ◽  
Natural Material ◽  
Propolis Extract

Introduction. Propolis (bee glue) is a natural product collected by honeybees from buds of various trees, shrubs and other plant species. Extracts of propolis possess numerous biological activities, including antioxidant, antibacterial, antifungal and anticancer. For this reason, propolis is currently used in many applications, such as preparations for cold syndrome, dermatological preparations or as a constituent of nutritional supplements and health food. The chemical composition of this natural material is very complex and depending on many factors, including method of extraction and selection of the solvent for the extraction process. Aim. The aim of the study was to determine concentration of selected phenolic compounds (flavonoids and phenolic acids) in extract of Polish propolis and estimate its antioxidant activity and effect on human red blood cells. Material and methods. In the propolis extract was determined concentration of 14 flavonoids and 9 phenolic acids using ultra-performance liquid chromatography equipped with a photodiode detector and a triple quadrupole mass spectrometer. The antioxidant potential of propolis extract was evaluated applying DPPH˙ free radical scavenging activity assay and Fe3+ reducing power assay. Moreover, the cytotoxicity and cytoprotective potential of propolis extract was estimated using human erythrocytes in vitro. Results. The propolis extract contained high concentration of pinocembrin, galangin, chrysin, apigenin, kaempferol, coumaric acid and cinnamic acid. It exhibited also high antioxidant potential. The antiradical activity of examined propolis extract was equal to 75% approx. activity of both standard antioxidants used in the study, namely Trolox and BHT. The reducing power of extract was equal to 65% approx. of Trolox and 80% of BHT, respectively. The propolis extract had no hemolytic activity, moreover, effectively protected human erythrocytes against free radicals-induced damage in vitro. Conclusions. The results of this study indicate that the propolis extract of national origin is a rich source of flavonoids and phenolic acids. Therefore, the propolis extract possesses a high antioxidant potential and can protect erythrocytes against free radicals-induced oxidative hemolysis.

Effects of oxygen free radicals on human erythrocytes: protective effects of deferoxamine and allopurinol

1990 ◽  
Vol 9 ◽  
pp. 33
Author(s):  
Moheb A. Rashid ◽  
Olof Jonsson ◽  
Donald G. Roberts ◽  
Göran William-Olsson
Keyword(s):  
Free Radicals ◽  
Oxygen Free Radicals ◽  
Human Erythrocytes ◽  
Protective Effects

Hexavalent chromium induces reactive oxygen species and impairs the antioxidant power of human erythrocytes and lymphocytes: Decreased metal reducing and free radical quenching ability of the cells

2017 ◽  
Vol 33 (8) ◽  
pp. 623-635 ◽  
Author(s):  
Nazim Husain ◽  
Riaz Mahmood
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Free Radicals ◽  
Free Radical ◽  
Hexavalent Chromium ◽  
Reactive Oxygen ◽  
Human Erythrocytes ◽  
Cellular Damage ◽  
Oxygen Species ◽  
Antioxidant Power

The toxicity of hexavalent chromium [Cr(VI)] in biological systems is thought to be closely associated with the generation of free radicals and reactive oxygen species. These species are produced when Cr(VI) is reduced to its trivalent form in the cell. This process results in oxidative stress due to an imbalance between the detoxifying ability of the cell and the production of free radicals. We have studied the effect of potassium dichromate (K2Cr2O7), a [Cr(VI)] compound, on the antioxidant power of human erythrocytes and lymphocytes under in vitro conditions. Incubation of erythrocytes and lymphocytes with different concentrations of K2Cr2O7 resulted in a marked dose-dependent decrease in reduced glutathione and an increase in oxidized glutathione and reactive oxygen species levels. The antioxidant power of the cells was decreased, as determined by metal reducing and free radical quenching assays. These results show that [Cr(VI)] upregulates the generation of reactive oxygen species and, as a consequence, the cellular antioxidant defences are compromised. The resulting oxidative stress may contribute to Cr(VI)-induced cellular damage.

scholarly journals Complexity of free radical Metabolism in human Erythrocytes

2010 ◽  
Vol 29 (3) ◽  
pp. 189-195 ◽  
Author(s):  
Aleksandra Nikolić-Kokić ◽  
Duško Blagojević ◽  
Mihajlo Spasić
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Superoxide Anion ◽  
Anion Radical ◽  
De Novo ◽  
Human Erythrocytes ◽  
Low Molecular Weight ◽  
Valuable Data ◽  
Vitamins E And C ◽  
Free Radical Metabolism

Complexity of free radical Metabolism in human ErythrocytesThe auto-oxidation of oxyhaemoglobin to methaemoglobin generating superoxide anion radical (O2.-) represents the main source of free radicals in the erythro-cytes. Hydrogen peroxide is produced by O2.-dismutation or originates from the circulation. Human erythrocytes are also exposed to the prooxidative actions of nitric oxide (NO) from circulation. Free radicals that may induce reactions with direct dangerous consequences to erythrocytes are also preceded by the reaction of O2.-and NO producing peroxynitrite. In physiological settings, erythrocytes show a self-sustaining activity of antioxidative defence (AD) enzymes, such as: superoxide dismutase (SOD, EC 1.11.16), catalase (CAT, EC 1.11.1.6), glutathione peroxidase (GSHPx, EC 1.11.1.9) and glutathione reductase (GR, EC 1.6.4.2), as well as low molecular weight antioxidants: glutathione and vitamins E and C. Their coordinate actions protect the erythrocyte's bio-macromolecules from free radical-mediated damage. Since there is node novosynthesis of AD enzymes in mature erythrocytes, their defence capacity is limited. Free radicals influence antioxidative enzymes capacities and relative share of particular components in the whole antioxidative system. Therefore, by measuring changes in the activity of individual AD components, as well as their interrelations by statistical canonical discriminant methods, valuable data about the complexity, overall relations and coordinated actions in the AD system in erythrocytes and its relevance for systemic effects can be acquired.

Two Mechanisms for Toxic Effects of Hydroxylamines in Human Erythrocytes: Involvement of Free Radicals and Risk of Potentiation

1998 ◽  
Vol 24 (3) ◽  
pp. 280-295 ◽  
Author(s):  
Chris T.A. Evelo ◽  
Anita A.M.G Spooren ◽  
Rob A.G. Bisschops ◽  
Leo G.M. Baars ◽  
John M. Neis
Keyword(s):  
Free Radicals ◽  
Human Erythrocytes ◽  
Toxic Effects

Elemental analysis of human erythrocytes

1989 ◽  
Vol 47 ◽  
pp. 242-243
Author(s):  
S. A. Livesey ◽  
A. A. del Campo ◽  
E. S. Griffey ◽  
D. Ohlmer ◽  
T. Schifani ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Elemental Analysis ◽  
Human Erythrocyte ◽  
Spectroscopic Analysis ◽  
Model System ◽  
Human Erythrocytes ◽  
List Type ◽  
Chemical Fixation ◽  
Ethanol Dehydration ◽  
Lowicryl K4m

The aim of this study is to compare methods of sample preparation for elemental analysis. The model system which is used is the human erythrocyte. Energy dispersive spectroscopic analysis has been previously reported for cryofixed and cryosectioned erythrocytes. Such work represents the reference point for this study. The use of plastic embedded samples for elemental analysis has also been documented. The work which is presented here is based on human erythrocytes which have been either chemically fixed and embedded or cryofixed and subsequently processed by a variety of techniques which culminated in plastic embedded samples.Heparinized and washed erythrocytes were prepared by the following methods for this study :(1). Chemical fixation in 4% paraformaldehyde/0.25% glutaraldehyde/0.2 M sucrose in 0.1 M Na cacodylate, pH 7.3 for 30 min, followed by ethanol dehydration, infiltration and embedding in Lowicryl K4M at -20° C.

Sign in / Sign up

Export Citation Format

Share Document