Basic functional analysis of six unknown open reading frames fromSaccharomyces cerevisiae: four from chromosome VII and two from chromosome XV

Yeast ◽  
2002 ◽  
Vol 19 (2) ◽  
pp. 123-129 ◽  
Author(s):  
Bruce M. Pearson ◽  
Michael Schweizer
Keyword(s):  
Functional Analysis ◽  
Open Reading Frames ◽  
Reading Frames

Sequencing and functional analysis of a 32 560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including theKIP1 andSEC17 genes

Yeast ◽  
1993 ◽  
Vol 9 (12) ◽  
pp. 1355-1371 ◽  
Author(s):  
Bart Scherens ◽  
Mohamed El Bakkoury ◽  
Fabienne Vierendeels ◽  
Evelyne Dubois ◽  
Francine Messenguy
Keyword(s):  
Functional Analysis ◽  
Open Reading Frames ◽  
Yeast Chromosome ◽  
Chromosome Ii ◽  
Reading Frames

Prediction and functional analysis of the replication origin of the linear plasmid pSCL2 inStreptomyces clavuligerus

2006 ◽  
Vol 52 (4) ◽  
pp. 293-300 ◽  
Author(s):  
Wei Wu ◽  
Shannon K. D Leblanc ◽  
James Piktel ◽  
Susan E Jensen ◽  
Kenneth L Roy
Keyword(s):  
Amino Acids ◽  
Functional Analysis ◽  
Replication Origin ◽  
Streptomyces Clavuligerus ◽  
Replication Initiation ◽  
Open Reading Frames ◽  
Linear Plasmid ◽  
Replication Proteins ◽  
Bacterial Plasmid ◽  
Reading Frames

pSCL2 (120 kb), one of the linear plasmids found in Streptomyces clavuligerus NRRL3585, was isolated and partially sequenced. Computational analysis of the central region of pSCL2 revealed the presence of two open reading frames that appear to encode proteins highly homologous to RepL1 and RepL2, replication proteins from pSLA2-L, the large linear plasmid in Streptomyces rochei. The S. clavuligerus open reading frames were designated repC1 and repC2, encoding the proteins RepC1 (150 amino acids) and RepC2 (102 amino acids), respectively. The RepC and RepL proteins have identical translation features and very similar predicted secondary and tertiary structures. Functional analysis confirmed that RepC1 is essential for replication initiation of pSCL2, whereas RepC2 is dispensable but may play a role in copy number control. The RepC and RepL proteins do not show similarity to any other bacterial plasmid replication proteins. Three regions of DNA sequence, Box 1 (1050–850 bp), Box 2 (723–606 bp), and Box 3 (224–168 bp), located upstream of repC1, were also shown to be essential or very important for replication of pSCL2.Key words: pSCL2, Streptomyces clavuligerus, replication origin.

Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 32-42 ◽  
Author(s):  
B. F. Francis Ouellette ◽  
Michael W. Clark ◽  
Teresa Keng ◽  
Reg K. Storms ◽  
Wuwei Zhong ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Functional Analysis ◽  
Genome Analysis ◽  
Open Reading Frames ◽  
Average Spacing ◽  
Coding Regions ◽  
Significant Homology ◽  
Yeast Chromosome ◽  
Chromosome I ◽  
Reading Frames

The DNA sequencing and preliminary functional analysis of a 32 kb section of yeast chromosome I has been completed. This region lies on the left arm of the chromosome between the LTE1 and SPO7 genes and contains 14 open reading frames (ORFs) positioned closely together, with an average spacing of approximately 350 nucleotides between coding regions. Three of these ORFs correspond to previously identified genes, a further three show significant homology with other proteins, while the remaining eight ORFs share no significant homology to genes in the databases.Key words: chromosome I, genome analysis, Saccharomyces cerevisiae, yeast.

Functional analysis of eight open reading frames on chromosomes XII and XIV ofSaccharomyces cerevisiae

Yeast ◽  
2000 ◽  
Vol 16 (16) ◽  
pp. 1457-1468 ◽  
Author(s):  
Shakeel Ahmed Khan ◽  
Nianshu Zhang ◽  
Thamir Ismail ◽  
Abdel-Nasser El-Moghazy ◽  
Amna Butt ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Open Reading Frames ◽  
Reading Frames

Functional analysis of three adjacent open reading frames from the right arm of yeast chromosome XVI

Yeast ◽  
1998 ◽  
Vol 14 (11) ◽  
pp. 1027-1039 ◽  
Author(s):  
Beata Waśkiewicz-Staniorowska ◽  
Jacek Skała ◽  
Michał Jasiński ◽  
Marcelle Grenson ◽  
André Goffeau ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Open Reading Frames ◽  
Yeast Chromosome ◽  
The Right ◽  
Reading Frames
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