Cloning of glycerol-3-phosphate dehydrogenase genes (ZrGPD1 andZrGPD2) and glycerol dehydrogenase genes (ZrGCY1 andZrGCY2) from the salt-tolerant yeastZygosaccharomyces rouxii

Yeast ◽  
2001 ◽  
Vol 18 (8) ◽  
pp. 737-744 ◽  
Author(s):  
Tomoko Iwaki ◽  
Sachko Kurono ◽  
Yuki Yokose ◽  
Kenji Kubota ◽  
Youichi Tamai ◽  
...  
Keyword(s):  
Glycerol Dehydrogenase ◽  
Salt Tolerant ◽  
Glycerol 3 Phosphate Dehydrogenase

Osmoregulation in Dunaliella. Intracellular Distribution of Enzymes of Glycerol Metabolism

1982 ◽  
Vol 37 (11-12) ◽  
pp. 1115-1123 ◽  
Author(s):  
A. D. Brown ◽  
R. McC. Lilley ◽  
Thérèse Marengo
Keyword(s):  
Mechanical Damage ◽  
Membrane System ◽  
Chloroplast Envelope ◽  
Glycerol Dehydrogenase ◽  
Glycerol Metabolism ◽  
Ribulose Bisphosphate Carboxylase ◽  
Ribulose Bisphosphate ◽  
Glycerol Phosphate ◽  
Bisphosphate Carboxylase ◽  
Glycerol 3 Phosphate Dehydrogenase

Abstract Dunaliella tertiolecta was disrupted mechanically and resolved by centrifugation into chloro­plast- and cytosol-enriched fractions. Intact chloroplasts could not be isolated because peripheral extensions of the single large chloroplast reached almost to the flagellar pole of the cell. The chloroplast envelope was closely appressed to the plasmalemma and, because of this and its dimensions, was vulnerable to mechanical damage to the cell. Distribution of enzymes of the glycerol cycle between the two fractions was compared with that of two marker enzymes, phosphoenolpyruvate carboxylase (cytosol) and ribulose bisphosphate carboxylase (chloroplast). The two reversible steps of the cycle were found to be spatially separated; glycerol-3-phosphate dehydrogenase (NAD-specific) was located in the chloroplast whereas glycerol dehydrogenase (NADP-specific) was located in the cytosol. The distribution of the two irreversible enzymes, glycerol phosphate phosphatase and dihydroxyacetone kinase is uncertain. These enzymes might occur about equally in both major compartments (cytoplasm and chloroplast) or be mitochondrial or they might be loosely associated with a membrane system. Implications of these results for regulation of the glycerol cycle are discussed.

scholarly journals Control of glycerol biosynthesis under high salt stress in Arabidopsis

2014 ◽  
Vol 41 (1) ◽  
pp. 87 ◽  
Author(s):  
Ahmed Bahieldin ◽  
Jamal S. M. Sabir ◽  
Ahmed Ramadan ◽  
Ahmed M. Alzohairy ◽  
Rania A. Younis ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Glycerol Dehydrogenase ◽  
Growth Stages ◽  
Loss Of Function ◽  
Physiological Importance ◽  
Key Enzyme ◽  
Dna Insertion ◽  
Glycerol 3 Phosphate Dehydrogenase ◽  
Gpd1 Gene

Loss-of-function and gain-of-function approaches were utilised to detect the physiological importance of glycerol biosynthesis during salt stress and the role of glycerol in conferring salt tolerance in Arabidopsis. The salt stress experiment involved wild type (WT) and transgenic Arabidopsis overexpressing the yeast GPD1 gene (analogue of Arabidopsis GLY1 gene). The experiment also involved the Arabidopsis T-DNA insertion mutants gly1 (for suppression of glycerol 3-phosphate dehydrogenase or G3PDH), gli1 (for suppression of glycerol kinase or GK), and act1 (for suppression of G3P acyltransferase or GPAT). We evaluated salt tolerance levels, in conjunction with glycerol and glycerol 3-phosphate (G3P) levels and activities of six enzymes (G3PDH, ADH (alcohol dehydrogenase), ALDH (aldehyde dehydrogenase), GK, G3PP (G3P phosphatase) and GLYDH (glycerol dehydrogenase)) involved in the glycerol pathway. The GPD1 gene was used to overexpress G3PDH, a cytosolic NAD+-dependent key enzyme of cellular glycerol biosynthesis essential for growth of cells under abiotic stresses. T2 GPD1-transgenic plants and those of the two mutants gli1 and act1 showed enhanced salt tolerance during different growth stages as compared with the WT and gly1 mutant plants. These results indicate that the participation of glycerol, rather than G3P, in salt tolerance in Arabidopsis. The results also indicate that the gradual increase in glycerol levels in T2 GPD1-transgenic, and gli1 and act1 mutant plants as NaCl level increases whereas they dropped at 200 mM NaCl. However, the activities of the G3PDH, GK, G3PP and GLYDH at 150 and 200 mM NaCl were not significantly different. We hypothesise that mechanism(s) of glycerol retention/efflux in the cell are affected at 200 mM NaCl in Arabidopsis.

Application of SSR Technique for the Identification of Markers Linked to Salinity Tolerance in Rice

2013 ◽  
Vol 19 (2) ◽  
pp. 57-65
Author(s):  
MH Kabir ◽  
MM Islam ◽  
SN Begum ◽  
AC Manidas
Keyword(s):  
Salt Tolerance ◽  
Ssr Markers ◽  
Salinity Tolerance ◽  
Seedling Stage ◽  
Phenotypic Screening ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Landrace ◽  
Salt Susceptible

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008

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