II. Yeast sequencing reports. The sequence of an 8·8 kb segment on the left arm of chromosome II fromSaccharomyces cerevisiae reveals four new open reading frames including homologs of animal DNA polymerase α-primases and bacterial GTP cyclohydrolase II

Yeast ◽  
1994 ◽  
Vol 10 (S1994A) ◽  
pp. S13-S24 ◽  
Author(s):  
Luc van Dyck ◽  
Bénédicte Purnelle ◽  
André Goffeau ◽  
Jacek Skala
Keyword(s):  
Dna Polymerase ◽  
Open Reading Frames ◽  
Gtp Cyclohydrolase ◽  
Dna Polymerase Α ◽  
Gtp Cyclohydrolase Ii ◽  
Chromosome Ii ◽  
Reading Frames

scholarly journals A Novel DNA Polymerase Family Found inArchaea

1998 ◽  
Vol 180 (8) ◽  
pp. 2232-2236 ◽  
Author(s):  
Yoshizumi Ishino ◽  
Kayoko Komori ◽  
Isaac K. O. Cann ◽  
Yosuke Koga
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Pyrococcus Furiosus ◽  
Dna Polymerases ◽  
Open Reading Frames ◽  
Gene Products ◽  
Polymerase Gene ◽  
Hyperthermophilic Archaeon ◽  
Dna Polymerase Ii ◽  
Reading Frames

ABSTRACT One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase-like open reading frames (ORFs) were found besides one ORF having the typical α-like DNA polymerase (family B). Recently, we identified the genes of DNA polymerase II (the second DNA polymerase) from the hyperthermophilic archaeonPyrococcus furiosus, which has also at least one α-like DNA polymerase (T. Uemori, Y. Sato, I. Kato, H. Doi, and Y. Ishino, Genes Cells 2:499–512, 1997). The genes in M. jannaschiiencoding the proteins that are homologous to the DNA polymerase II ofP. furiosus have been located and cloned. The gene products of M. jannaschii expressed in Escherichia colihad both DNA polymerizing and 3′→5′ exonuclease activities. We propose here a novel DNA polymerase family which is entirely different from other hitherto-described DNA polymerases.

Sequencing and functional analysis of a 32 560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including theKIP1 andSEC17 genes

Yeast ◽  
1993 ◽  
Vol 9 (12) ◽  
pp. 1355-1371 ◽  
Author(s):  
Bart Scherens ◽  
Mohamed El Bakkoury ◽  
Fabienne Vierendeels ◽  
Evelyne Dubois ◽  
Francine Messenguy
Keyword(s):  
Functional Analysis ◽  
Open Reading Frames ◽  
Yeast Chromosome ◽  
Chromosome Ii ◽  
Reading Frames

Sequence analysis of a 5·6 kb fragment of chromosome II fromSaccharomyces cerevisiae reveals two new open reading frames next toCDC28

Yeast ◽  
1995 ◽  
Vol 11 (5) ◽  
pp. 455-458 ◽  
Author(s):  
Sabine Baur ◽  
Jûrgen Becker ◽  
Ziyu Li ◽  
Eckhard Niegemann ◽  
Eugen Wehner ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Open Reading Frames ◽  
Chromosome Ii ◽  
Reading Frames

scholarly journals Human GTP cyclohydrolase I: only one out of three cDNA isoforms gives rise to the active enzyme

1994 ◽  
Vol 302 (1) ◽  
pp. 215-221 ◽  
Author(s):  
M Gütlich ◽  
E Jaeger ◽  
K P Rücknagel ◽  
T Werner ◽  
W Rödl ◽  
...  
Keyword(s):  
Maximum Activity ◽  
Open Reading Frames ◽  
Intermediate Step ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase ◽  
Reading Frame ◽  
Cdna Sequences ◽  
Rate Limiting Step ◽  
Enzyme Isoforms ◽  
Reading Frames

GTP cyclohydrolase I catalyses the first and rate-limiting step of tetrahydrobiopterin biosynthesis. Its expression is regulated by interferon-gamma or kit ligand in a tissue-specific manner. Three different cDNA forms have been reported for human GTP cyclohydrolase I [Togari, Ichinose, Matsumoto, Fujita and Nagatsu (1992) Biochem. Biophys. Res. Commun. 187, 359-365]. We have isolated, from a human liver cDNA library, two clones which contained inserts identical with two of the cDNAs reported by Togari et al. (1992). The three open reading frames corresponding to all reported cDNA sequences were expressed in Escherichia coli. Only the recombinant protein corresponding to the longest reading frame catalysed the conversion of GTP into dihydroneopterin triphosphate. The proteins corresponding to the shorter reading frames failed to catalyse not only the generation of dihydroneopterin triphosphate but also the release of formate from GTP, an intermediate step of the reaction. Recombinant human GTP cyclohydrolase I showed sigmoidal substrate kinetics and maximum activity at 60 degrees C. These findings are well in line with the published properties of the enzyme isolated from rat liver. The data indicate that cytokine-mediated induction of GTP cyclohydrolase I is not due to the expression of enzyme isoforms.

scholarly journals Erratum to: Sequence of a 4.8 kb fragment ofSaccharomyces cerevisiae chromosome II including three essential open reading frames

Yeast ◽  
1994 ◽  
Vol 10 (1) ◽  
pp. 131-131 ◽  
Author(s):  
Axel Baur ◽  
Ine Schaaff-Gerstenschläger ◽  
Eckhard Boles ◽  
Thomas Miosga ◽  
Matthias Rose ◽  
...  
Keyword(s):  
Open Reading Frames ◽  
Chromosome Ii ◽  
Reading Frames

Sequence of a 4·8 kb fragment ofSaccharomyces cerevisiae chromosome II including three essential open reading frames

Yeast ◽  
1993 ◽  
Vol 9 (3) ◽  
pp. 289-293 ◽  
Author(s):  
Axel Baur ◽  
Ine Schaaff-Gerstenschläger ◽  
Eckhard Boles ◽  
Thomas Miosga ◽  
Matthias Rose ◽  
...  
Keyword(s):  
Open Reading Frames ◽  
Chromosome Ii ◽  
Reading Frames
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