Purification of δ-aminolevulinate dehydratase from genetically engineered yeast

Yeast ◽  
1990 ◽  
Vol 6 (4) ◽  
pp. 319-330 ◽  
Author(s):  
Leda M. Borralho ◽  
Claudio H. D. Ortiz ◽  
Anita D. Panek ◽  
James R. Mattoon
Keyword(s):  
Genetically Engineered ◽  
Engineered Yeast ◽  
Aminolevulinate Dehydratase

Ethanol production from corn cob pretreated by the ammonia steeping process using genetically engineered yeast

1996 ◽  
Vol 18 (9) ◽  
pp. 1013-1018 ◽  
Author(s):  
N. J. Cao ◽  
M. S. Krishnan ◽  
J. X. Du ◽  
C. S. Gong ◽  
N. W. Y. Ho ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Genetically Engineered ◽  
Corn Cob ◽  
Engineered Yeast ◽  
Ammonia Steeping

Novo human insulin - the same but different

1990 ◽  
Vol 28 (6) ◽  
pp. 24-24
Keyword(s):  
Human Insulin ◽  
Manufacturing Process ◽  
Genetically Engineered ◽  
Yeast Cells ◽  
Enzymatic Modification ◽  
Engineered Yeast ◽  
Direct Biosynthesis

Unannounced to prescribers or patients, Novo Nordisk have changed the manufacturing process of their human insulins from enzymatic modification of pig insulin (emp) to direct biosynthesis of genetically engineered yeast cells (pyr). The preservative for Human Actrapid insulin has also been changed from phenol to meta-cresol. No warning of this change was sent to pharmacists, doctors, diabetes specialists or patients.

Overexpression of an Inulinase Gene in an Oleaginous Yeast, Aureobasidium melanogenum P10, for Efficient Lipid Production from Inulin

2018 ◽  
Vol 28 (4) ◽  
pp. 190-200 ◽  
Author(s):  
Yan-Feng Li ◽  
Hong Jiang ◽  
Zhong Hu ◽  
Guang-Lei Liu ◽  
Zhen-Ming Chi ◽  
...  
Keyword(s):  
Lipid Production ◽  
Dry Weight ◽  
Genetically Engineered ◽  
Biodiesel Production ◽  
Yeast Cells ◽  
Gene Encoding ◽  
Direct Production ◽  
Inulinase Gene ◽  
Inulinase Activity ◽  
Engineered Yeast

In this study, in order to directly and efficiently convert inulin into a single-cell oil (SCO), an <i>INU1</i> gene encoding inulinase from<b><i></i></b> <i>Kluyveromyces marxianus</i> was integrated into the genomic DNA and actively expressed in an SCO producer <i>Aureobasidium</i> <i>melanogenum</i> P10. The transformant API41 obtained produced 28.5 U/mL of inulinase and its wild-type strain P10 yielded only 8.62 U/mL. Most (97.5%) of the inulinase produced by the transformant API41 was secreted into the culture. During a 10-L fermentation, 66.2% (w/w) lipid in the yeast cells of the transformant API41 and 14.38 g/L of cell dry weight were attained from inulin of 80.0 g/L within 120 h, high inulinase activity (23.7 U/mL) was also produced within 72 h, and the added inulin was actively hydrolyzed. This confirmed that the genetically engineered yeast of <i>A. melanogenum</i> P10 is suitable for direct production of lipids from inulin. The lipids produced could be used as feedstocks for biodiesel production.

Detection of hormone active chemicals using genetically engineered yeast cells and microfluidic devices with interdigitated array electrodes

2009 ◽  
Vol 30 (19) ◽  
pp. 3406-3412 ◽  
Author(s):  
Kosuke Ino ◽  
Yusuke Kitagawa ◽  
Tsuyoshi Watanabe ◽  
Hitoshi Shiku ◽  
Masahiro Koide ◽  
...  
Keyword(s):  
Microfluidic Devices ◽  
Genetically Engineered ◽  
Yeast Cells ◽  
Interdigitated Array Electrodes ◽  
Engineered Yeast ◽  
Interdigitated Array

Genetically engineered yeast makes medicinal plant products

Nature ◽  
2020 ◽  
Vol 585 (7826) ◽  
pp. 504-505
Author(s):  
José Montaño López ◽  
José L. Avalos
Keyword(s):  
Medicinal Plant ◽  
Genetically Engineered ◽  
Plant Products ◽  
Engineered Yeast
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