Detection of hormone active chemicals using genetically engineered yeast cells and microfluidic devices with interdigitated array electrodes

2009 ◽  
Vol 30 (19) ◽  
pp. 3406-3412 ◽  
Author(s):  
Kosuke Ino ◽  
Yusuke Kitagawa ◽  
Tsuyoshi Watanabe ◽  
Hitoshi Shiku ◽  
Masahiro Koide ◽  
...  
Keyword(s):  
Microfluidic Devices ◽  
Genetically Engineered ◽  
Yeast Cells ◽  
Interdigitated Array Electrodes ◽  
Engineered Yeast ◽  
Interdigitated Array

Novo human insulin - the same but different

1990 ◽  
Vol 28 (6) ◽  
pp. 24-24
Keyword(s):  
Human Insulin ◽  
Manufacturing Process ◽  
Genetically Engineered ◽  
Yeast Cells ◽  
Enzymatic Modification ◽  
Engineered Yeast ◽  
Direct Biosynthesis

Unannounced to prescribers or patients, Novo Nordisk have changed the manufacturing process of their human insulins from enzymatic modification of pig insulin (emp) to direct biosynthesis of genetically engineered yeast cells (pyr). The preservative for Human Actrapid insulin has also been changed from phenol to meta-cresol. No warning of this change was sent to pharmacists, doctors, diabetes specialists or patients.

Overexpression of an Inulinase Gene in an Oleaginous Yeast, Aureobasidium melanogenum P10, for Efficient Lipid Production from Inulin

2018 ◽  
Vol 28 (4) ◽  
pp. 190-200 ◽  
Author(s):  
Yan-Feng Li ◽  
Hong Jiang ◽  
Zhong Hu ◽  
Guang-Lei Liu ◽  
Zhen-Ming Chi ◽  
...  
Keyword(s):  
Lipid Production ◽  
Dry Weight ◽  
Genetically Engineered ◽  
Biodiesel Production ◽  
Yeast Cells ◽  
Gene Encoding ◽  
Direct Production ◽  
Inulinase Gene ◽  
Inulinase Activity ◽  
Engineered Yeast

In this study, in order to directly and efficiently convert inulin into a single-cell oil (SCO), an <i>INU1</i> gene encoding inulinase from<b><i></i></b> <i>Kluyveromyces marxianus</i> was integrated into the genomic DNA and actively expressed in an SCO producer <i>Aureobasidium</i> <i>melanogenum</i> P10. The transformant API41 obtained produced 28.5 U/mL of inulinase and its wild-type strain P10 yielded only 8.62 U/mL. Most (97.5%) of the inulinase produced by the transformant API41 was secreted into the culture. During a 10-L fermentation, 66.2% (w/w) lipid in the yeast cells of the transformant API41 and 14.38 g/L of cell dry weight were attained from inulin of 80.0 g/L within 120 h, high inulinase activity (23.7 U/mL) was also produced within 72 h, and the added inulin was actively hydrolyzed. This confirmed that the genetically engineered yeast of <i>A. melanogenum</i> P10 is suitable for direct production of lipids from inulin. The lipids produced could be used as feedstocks for biodiesel production.

Genetically engineered yeast cells and their applications

1995 ◽  
Vol 82-83 ◽  
pp. 815-822 ◽  
Author(s):  
Denis Pompon ◽  
Alain Perret ◽  
Aouatef Bellamine ◽  
Romuald Laine ◽  
Jean-Charles Gautier ◽  
...  
Keyword(s):  
Genetically Engineered ◽  
Yeast Cells ◽  
Engineered Yeast

Enhanced arsenic accumulation by engineered yeast cells expressingArabidopsis thaliana phytochelatin synthase

2007 ◽  
Vol 99 (2) ◽  
pp. 333-340 ◽  
Author(s):  
Shailendra Singh ◽  
Wonkyu Lee ◽  
Nancy A. DaSilva ◽  
Ashok Mulchandani ◽  
Wilfred Chen
Keyword(s):  
Yeast Cells ◽  
Phytochelatin Synthase ◽  
Arsenic Accumulation ◽  
Engineered Yeast

Purification of δ-aminolevulinate dehydratase from genetically engineered yeast

Yeast ◽  
1990 ◽  
Vol 6 (4) ◽  
pp. 319-330 ◽  
Author(s):  
Leda M. Borralho ◽  
Claudio H. D. Ortiz ◽  
Anita D. Panek ◽  
James R. Mattoon
Keyword(s):  
Genetically Engineered ◽  
Engineered Yeast ◽  
Aminolevulinate Dehydratase

Microbial fluorescence sensing for human neurotensin receptor type 1 using Gα-engineered yeast cells

2014 ◽  
Vol 446 ◽  
pp. 37-43 ◽  
Author(s):  
Jun Ishii ◽  
Asami Oda ◽  
Shota Togawa ◽  
Akira Fukao ◽  
Toshinobu Fujiwara ◽  
...  
Keyword(s):  
Yeast Cells ◽  
Receptor Type ◽  
Fluorescence Sensing ◽  
Neurotensin Receptor ◽  
Engineered Yeast
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