Orthogonal-field-alternation gel electrophoresis banding patterns of DNA from yeasts

Yeast ◽  
1986 ◽  
Vol 2 (3) ◽  
pp. 193-204 ◽  
Author(s):  
Peter De Jonge ◽  
Francien C. M. De Jongh ◽  
Robert Meijers ◽  
H. Yde Steensma ◽  
W. Alexander Scheffers
Keyword(s):  
Gel Electrophoresis ◽  
Banding Patterns ◽  
Orthogonal Field

Assignment of cloned genes to the seven electrophoretically separated Candida albicans chromosomes

1988 ◽  
Vol 8 (11) ◽  
pp. 4721-4726
Author(s):  
B B Magee ◽  
Y Koltin ◽  
J A Gorman ◽  
P T Magee
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Albicans ◽  
Unknown Function ◽  
Gel Electrophoresis ◽  
Homogeneous Field ◽  
Electrophoretic Karyotype ◽  
Chromosome Size ◽  
Homologous Chromosomes ◽  
Orthogonal Field ◽  
Chromosomal Bands

By using orthogonal-field alternating gel electrophoresis (OFAGE), field-inversion gel electrophoresis (FIGE), and contour-clamped homogeneous field gel electrophoresis (CHEF), we have clearly resolved 11 chromosomal bands from various Candida albicans strains. OFAGE resolves the smaller chromosomes better, while FIGE, which under our conditions causes the chromosomes to run in the reverse order of OFAGE, is more effective in separating the larger chromosomes. CHEF separates all chromosomes under some conditions, but these conditions do not often resolve homologs. The strains examined are highly polymorphic for chromosome size. Fourteen cloned Candida genes, isolated on the basis of conferral of new properties to or complementation of auxotrophic deficiencies in Saccharomyces cerevisiae, and three sequences of unknown function have been hybridized to Southern transfers of CHEF, FIGE, and OFAGE gels. Four sets of resolvable bands have been shown to be homologous chromosomes. On the basis of these data, we suggest that C. albicans has seven chromosomes. Genes have been assigned to the seven chromosomes. Two chromosomes identified genetically have been located on the electrophoretic karyotype.

Genetic diversity in red pine: evidence for low genic heterozygosity

1977 ◽  
Vol 7 (2) ◽  
pp. 343-347 ◽  
Author(s):  
D. P. Fowler ◽  
R. W. Morris
Keyword(s):  
Genetic Diversity ◽  
Gel Electrophoresis ◽  
Allozyme Variation ◽  
Red Pine ◽  
Starch Gel Electrophoresis ◽  
Banding Patterns ◽  
Coniferous Species ◽  
Low Degree ◽  
Starch Gel ◽  
Genetically Determined

Starch gel electrophoresis was used to survey for genetically determined enzyme mobility differences among 297 megagametophytes of red pine (Pinusresinosa Ait.) from five widely separated geographical sources. Consistent and reproducible enzyme banding patterns were observed with five of the seven isozyme systems assayed. No variation in band mobility was observed in any of these systems. This result stands in contrast with those reported from surveys of allozyme variation in other coniferous species but is consistent with the low degree of genetic variation observed in red pine for higher levels of genetic organization. It is concluded that red pine is genetically depauperate.Possible explanations for restricted genetic diversity are discussed. The most plausible explanation suggests that red pine was at sometime, possibly during the Pleistocene, reduced to a small refugial population and has yet to reestablish equilibrium heterozygosity.

Electrophoretic evidence supporting self-incompatibility in Lotus corniculatus

1980 ◽  
Vol 58 (6) ◽  
pp. 712-716 ◽  
Author(s):  
Shirley Dobrofsky ◽  
W. F. Grant
Keyword(s):  
Gel Electrophoresis ◽  
Seed Set ◽  
Polyacrylamide Gel Electrophoresis ◽  
Lotus Corniculatus ◽  
Polyacrylamide Gel ◽  
Self Incompatibility ◽  
Banding Patterns ◽  
Protein Subunits ◽  
Self Pollination

Self-incompatibility, a prefertilization event, and self-sterility, a postfertilization event, have both been suggested as causes for differences in seed set between cross- and self-pollinated florets in Lotus corniculatus L. Ovary protein subunits of selfed, crossed, and unpollinated florets of L. corniculatus cv. Mirabel were studied using polyacrylamide gel electrophoresis. Banding patterns differed for all three conditions. Ovary protein differences were found prior to the time fertilization is known to occur, thereby providing evidence that self-incompatibility is at least partially responsible for the reduced seed set after self-pollination.

Genetic diversity among Trypanosoma brucei rhodesiense isolates from Tanzania

Parasitology ◽  
1997 ◽  
Vol 115 (6) ◽  
pp. 571-579 ◽  
Author(s):  
E. K. KOMBA ◽  
S. N. KIBONA ◽  
A. K. AMBWENE ◽  
J. R. STEVENS ◽  
W. C. GIBSON
Keyword(s):  
Trypanosoma Brucei ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Surface Glycoprotein ◽  
East African ◽  
Trypanosoma Brucei Rhodesiense ◽  
Banding Patterns ◽  
Predominant Genotype ◽  
Length Polymorphisms ◽  
Random Amplification

We compared 19 stocks of Trypanosoma brucei rhodesiense collected in 1991 and 1994 from Tanzania with representative stocks from other foci of Rhodesian sleeping sickness in Zambia, Kenya and Uganda. Stocks were characterized by isoenzyme electrophoresis, restriction fragment length polymorphisms in variant surface glycoprotein genes and random amplification of polymorphic DNA; the banding patterns obtained were coded for numerical analysis. In addition, the Tanzanian stocks were compared by pulsed field gel electrophoresis. Overall the Tanzanian stocks formed a homogeneous group and the predominant genotype isolated in 1991 was still present in the 1994 sample, although at a reduced level. The Tanzanian stocks were distinct from representative stocks from other East African foci. This observation does not support the proposal that there are northern and southern strains of T. b. rhodesiense, but is consistent with the view that T. b. rhodesiense stocks form a mosaic of different genotypes varying from focus to focus in East Africa.

Donor-to-Recipient Transmission of Bacteria as an Unusual Cause of Mediastinitis in a Heart Transplant Recipient

1999 ◽  
Vol 20 (02) ◽  
pp. 132-133 ◽  
Author(s):  
Jeffrey S. Burket ◽  
Carol E. Chenoweth ◽  
Thomas L. Meyer ◽  
Neil L. Barg
Keyword(s):  
Transplant Recipient ◽  
Heart Transplant ◽  
Gel Electrophoresis ◽  
Pathogenic Bacteria ◽  
Klebsiella Oxytoca ◽  
Heart Transplant Recipient ◽  
Pulsed Field Gel Electrophoresis ◽  
Banding Patterns ◽  
Pulsed Field

AbstractWe present a 54-year-old male heart transplant recipient who developed mediastinitis caused byKlebsiella oxytocaandVeillonellaspecies. Culture of the donor's bronchus also grewK oxytocaand aVeillonellaspecies. Pulsed-field gel electrophoresis revealed that theK oxytocaisolates had identical banding patterns. This case illustrates transmission of pathogenic bacteria via a contaminated organ.

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