scholarly journals Making ends meet: New functions of mRNA secondary structure

2020 ◽  
Author(s):  
Dmitri N. Ermolenko ◽  
David H. Mathews
Keyword(s):  
Secondary Structure ◽  
Mrna Secondary Structure

scholarly journals Hypertrophic cardiomyopathy MYH7 mutation R723G alters mRNA secondary structure

2020 ◽  
Vol 52 (1) ◽  
pp. 15-19
Author(s):  
J. Rose ◽  
T. Kraft ◽  
B. Brenner ◽  
J. Montag
Keyword(s):  
Hypertrophic Cardiomyopathy ◽  
Secondary Structure ◽  
Experimental Evidence ◽  
Point Mutation ◽  
Shape Analysis ◽  
Allelic Imbalance ◽  
Mrna Secondary Structure ◽  
Wild Type ◽  
Mrna Structure

Point mutation R723G in the MYH7 gene causes hypertrophic cardiomyopathy (HCM). Heterozygous patients with this mutation exhibit a comparable allelic imbalance of the MYH7 gene. On average 67% of the total MYH7 mRNA are derived from the MYH7R723G-allele and 33% from the MYH7WT allele. Mechanisms underlying mRNA allelic imbalance are largely unknown. We suggest that a different mRNA lifetime of the alleles may cause the allelic drift in R723G patients. A potent regulator of mRNA lifetime is its secondary structure. To test for alterations in the MYH7R723G mRNA structure we used selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) analysis. We show significantly different SHAPE reactivity of wild-type and MYH7R723G RNA, which is in accordance with bioinformatically predicted structures. Thus, we provide the first experimental evidence for mRNA secondary structure alterations by the HCM point mutation. We assume that this may result in a prolonged lifetime of MYH7R723G mRNA in vivo and subsequently in the determined allelic imbalance.

The influence of mRNA secondary structure on expression of endoglucanase inBacillus subtilis

1994 ◽  
Vol 16 (10) ◽  
pp. 1001-1006 ◽  
Author(s):  
Baek-Rak Lee ◽  
Jeong Hyun Kim ◽  
M. Y. Pack
Keyword(s):  
Secondary Structure ◽  
Mrna Secondary Structure

Familial Alzheimer's mutation: mRNA secondary structure revisited

1992 ◽  
Vol 13 (3) ◽  
pp. 449-451 ◽  
Author(s):  
Jürgen Brosius ◽  
Danielle Konings
Keyword(s):  
Secondary Structure ◽  
Mrna Secondary Structure

Human Catechol-O-Methyltransferase Haplotypes Modulate Protein Expression by Altering mRNA Secondary Structure

Science ◽  
2006 ◽  
Vol 314 (5807) ◽  
pp. 1930-1933 ◽  
Author(s):  
A. G. Nackley ◽  
S. A. Shabalina ◽  
I. E. Tchivileva ◽  
K. Satterfield ◽  
O. Korchynskyi ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Protein Expression ◽  
Mrna Secondary Structure
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