Effects of Light and Water Disturbance on the Growth of Microcystis aeruginosa and the Release of Algal Toxins

2021 ◽  
Author(s):  
Shaofeng Xu ◽  
Lingyue Zhang ◽  
Kaizong Lin ◽  
Miao Bai ◽  
Yue Wang ◽  
...  
Keyword(s):  
Microcystis Aeruginosa ◽  
Algal Toxins

scholarly journals Valuable ingredients and feed toxicity evaluation of Microcystis aeruginosa acidolysis product in mice

2015 ◽  
Vol 240 (10) ◽  
pp. 1333-1339 ◽  
Author(s):  
Shiqun Han ◽  
Qing Zhou ◽  
Yudi Xu ◽  
Floris Vanogtrop ◽  
Qijin Guo ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Amino Acid ◽  
Hydrochloric Acid ◽  
Microcystis Aeruginosa ◽  
Micronucleus Test ◽  
Lethal Dose ◽  
Total Amino Acid ◽  
Algal Toxins ◽  
Eutrophic Lakes ◽  
Amino Acid Requirements

This research studied the extraction from Microcystis aeruginosa using hydrochloric acid method as a potentially valuable protein resource from eutrophic lakes. Amino acid composition, residual algal toxins, and heavy metals of the acidolysis product were studied. After 18 h of hydrochloric acid treatment, the product of M. aeruginosa contained 17 amino acids, 51.34% of total amino acid requirements, and 30.25% of the livestock and poultry essential amino acid (Eaa). The residual microcystin-LR (MC-LR) was 0.94 µg kg−1, which was less than WHO drinking water limit of microcystins. The removal ratio of microcystins was higher than 99.99% during the process of hydrolysis. The concentration of heavy metals of the product was in compliance with feed standards. Furthermore, using Horn’s method, Mouse Micronucleus Test and Sperm Shape Abnormality Test were conducted to study the forage safety of the product. Half lethal dose (LD50) of acidolysis product in mice was >9.09 g kg−1 body weight, actually belonging to non-toxic grade. Every dose treatment did not significantly increase activities of alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and γ-glutamyltransferase (γ-GT). The results of both micronucleus test and sperm shape abnormality test were negative, which suggested the product with no mutagenicity and sperm malformation effects. This study indicated that the acidolysis product of M. aeruginosa was safe to be used as a feed ingredient.

scholarly journals Allelopathic effect of Chitosan Fiber on the growth of Microcystis aeruginosa

2020 ◽  
Vol 206 ◽  
pp. 02012
Author(s):  
Jingguo Cao ◽  
Hongyan Zhao ◽  
Zezhang Dong ◽  
Zongzheng Yang
Keyword(s):  
Microcystis Aeruginosa ◽  
Cyanobacterial Bloom ◽  
Allelopathic Effect ◽  
Environment Protection ◽  
Inhibition Rate ◽  
Algal Toxins ◽  
Safety Protection ◽  
Novel Method ◽  
Chitosan Fiber ◽  
First Time

Cyanobacterial bloom elimination has become an urgent concern in aquatic environment protection. Allelopathic technology, however, is one of the potential effective approaches because of its exclusive and ecological safety protection. The potential application of chitosan fiber in the inhibition of Microcystis aeruginosa was explored for the first time. Results showed that the cell growth of M. aeruginosa was inhibited effectively by chitosan fibers. After 12 days of treatment, the inhibition rate (IR) of algal cells was above 78.9%; those of chlorophyll a and carotenoids in algal cells were 86.9% and 93.1%, respectively; those of phycocyanin, allophycocyanin and phycoerythrin in phycobiliproteins were all approximately 100%; those of soluble proteins and algal toxins were 88% and 100%, respectively; and those of superoxide dismutase and catalase activities were 68% and 74%, respectively. In addition, chitosan fiber effectively destroyed the structure of algal cells involved in the photosynthetic process, thereby destroying the metabolic activity of algal cells. This study provides a novel method utilizing a new allelopathic material.

Influence of increased nutrient supply on Microcystis aeruginosa at cellular and proteomic levels

2020 ◽  
Vol 85 ◽  
pp. 47-58
Author(s):  
Y Jiang ◽  
Y Liu
Keyword(s):  
Microcystis Aeruginosa ◽  
Regulatory Protein ◽  
Acid Synthesis ◽  
Nutrient Supply ◽  
Nitrogen Supply ◽  
Nitrogen And Phosphorus ◽  
Comprehensive Description ◽  
Amino Acid Synthesis ◽  
High Nutrient ◽  
Proteomic Responses

Various studies have observed that increased nutrient supply promotes the growth of bloom-forming cyanobacteria, but only a limited number of studies have investigated the influence of increased nutrient supply on bloom-forming cyanobacteria at the proteomic level. We investigated the cellular and proteomic responses of Microcystis aeruginosa to elevated nitrogen and phosphorus supply. Increased supply of both nutrients significantly promoted the growth of M. aeruginosa and the synthesis of chlorophyll a, protein, and microcystins. The release of microcystins and the synthesis of polysaccharides negatively correlated with the growth of M. aeruginosa under high nutrient levels. Overexpressed proteins related to photosynthesis, and amino acid synthesis, were responsible for the stimulatory effects of increased nutrient supply in M. aeruginosa. Increased nitrogen supply directly promoted cyanobacterial growth by inducing the overexpression of the cell division regulatory protein FtsZ. NtcA, that regulates gene transcription related to both nitrogen assimilation and microcystin synthesis, was overexpressed under the high nitrogen condition, which consequently induced overexpression of 2 microcystin synthetases (McyC and McyF) and promoted microcystin synthesis. Elevated nitrogen supply induced the overexpression of proteins involved in gas vesicle organization (GvpC and GvpW), which may increase the buoyancy of M. aeruginosa. Increased phosphorus level indirectly affected growth and the synthesis of cellular substances in M. aeruginosa through the mediation of differentially expressed proteins related to carbon and phosphorus metabolism. This study provides a comprehensive description of changes in the proteome of M. aeruginosa in response to an increased supply of 2 key nutrients.

Inhibitory effects of Cyperus alternifolius on growth of Microcystis aeruginosa and identification of algicidal substances

2019 ◽  
Vol 46 (1) ◽  
pp. 73-84
Author(s):  
L. Zhou ◽  
S. Nakai ◽  
G. F. Chen ◽  
Q. Pan ◽  
N. X. Cui ◽  
...  
Keyword(s):  
Microcystis Aeruginosa ◽  
Inhibitory Effects ◽  
Cyperus Alternifolius

Asymmetric warming enhances Microcystis aeruginosa resistance to macrophytic anti-cyanobacterial coumarin

2018 ◽  
Vol 43 (2) ◽  
pp. 265-274 ◽  
Author(s):  
W.X. Hong ◽  
S.P. Zuo ◽  
L.T. Ye ◽  
B.Q. Qin
Keyword(s):  
Microcystis Aeruginosa

scholarly journals Chemical Characterization of Microcystis aeruginosa for Feed and Energy Uses

Energies ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 3013
Author(s):  
Larissa Souza Passos ◽  
Éryka Costa Almeida ◽  
Claudio Martin Pereira de Pereira ◽  
Alessandro Alberto Casazza ◽  
Attilio Converti ◽  
...  
Keyword(s):  
Microcystis Aeruginosa ◽  
Animal Feed ◽  
Renewable Energy Sources ◽  
Chemical Characterization ◽  
Cyanobacterial Blooms ◽  
Energy Sources ◽  
Carbohydrate Source ◽  
Heating Value ◽  
Adverse Health Effects ◽  
Degradation Reactions

Cyanobacterial blooms and strains absorb carbon dioxide, drawing attention to its use as feed for animals and renewable energy sources. However, cyanobacteria can produce toxins and have a low heating value. Herein, we studied a cyanobacterial strain harvested during a bloom event and analyzed it to use as animal feed and a source of energy supply. The thermal properties and the contents of total nitrogen, protein, carbohydrate, fatty acids, lipid, and the presence of cyanotoxins were investigated in the Microcystis aeruginosa LTPNA 01 strain and in a bloom material. Microcystins (hepatotoxins) were not detected in this strain nor in the bloom material by liquid chromatography coupled to mass spectrometry. Thermogravimetric analysis showed that degradation reactions (devolatilization) initiated at around 180 °C, dropping from approximately 90% to 20% of the samples’ mass. Our work showed that despite presenting a low heating value, both biomass and non-toxic M. aeruginosa LTPNA 01 could be used as energy sources either by burning or producing biofuels. Both can be considered a protein and carbohydrate source similar to some microalgae species as well as biomass fuel. It could also be used as additive for animal feed; however, its safety and potential adverse health effects should be further investigated.

scholarly journals Effects of Prometryn Exposure Scenarios on Microcystis aeruginosa Growth and Water Qualities in Incubator Experiments

Water ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1346
Author(s):  
Zhenjiang Yang ◽  
Suiliang Huang ◽  
Xiaowen Huang ◽  
Xiaofu Liang ◽  
Waseem Akram ◽  
...  
Keyword(s):  
Microcystis Aeruginosa ◽  
Growth Rates ◽  
Biological Effects ◽  
High Dose ◽  
Single Exposure ◽  
Time Of Application ◽  
Maximum Inhibition ◽  
Nutrient Consumption ◽  
Exposure Pattern ◽  
Significant Factors

Although multiple herbicide exposures are more prospective to occur in water, many previous studies were carried out as single herbicide exposure. To investigate the toxic effect of prometryn on cyanobacteria and water qualities, single and double prometryn exposures (at different growth phases) on Microcystis aeruginosa growth and concentrations of nutrients were compared after a 44-day experiment. Results indicated that under single exposure, maximum inhibition rates were 4.7–12.0% higher than those under double exposures. Correspondingly, the maximum Microcystis aeruginosa densities and growth rates under single exposure were 10.3–21.1% and 19.5–37.7% lower than those under double exposures (p < 0.05), respectively. These findings revealed that repeated prometryn exposures resulted in a reduction in biological effects, because the time of application and the concentration injected during the first application were both significant factors in the biological effects of prometryn. Prometryn exposure scenarios did not have a significant effect on nutrient or nutrient consumption concentrations (p > 0.05). In general, the pattern of nutrient limitation showed a shift from phosphorus to nitrogen limitation. The quantified relationships between Microcystis aeruginosa growth rates and consumed nutrients were studied. Based on the above findings, we believe that a high-dose and single prometryn exposure is a more effective exposure pattern for limiting cyanobacteria growth.

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