Effect of centrifugation on water recycling and algal growth to enable algae biodiesel production

2019 ◽  
Author(s):  
Thomas Igou ◽  
Steven W. Van Ginkel ◽  
Patricia Penalver-Argueso ◽  
Hao Fu ◽  
Shusuke Doi ◽  
...  
2014 ◽  
Vol 86 (12) ◽  
pp. 2325-2329 ◽  
Author(s):  
Thomas Igou ◽  
Steven W. Van Ginkel ◽  
Patricia Penalver-Argueso ◽  
Hao Fu ◽  
Shusuke Doi ◽  
...  

2017 ◽  
Vol 19 ◽  
pp. 331-337 ◽  
Author(s):  
Kosar Mozaffarikhah ◽  
Ali Kargari ◽  
Meisam Tabatabaei ◽  
Hossein Ghanavati ◽  
Mohammad Mahdi A. Shirazi

In current scenario algal fuel is considered as the most viable solution for the depleting non-renewable fuel sources. This study demonstrates the controlled, continuous production of the microalgae species Chlorella Pyrenoidosa in a novel designed hybrid photobioreactor aimed to the later production of biodiesel. The reactor is operated at different light intensity and its effect on the biomass production and pH was assessed. The final biomass is harvested by centrifugation preceded by sedimentation from which the algal lipids are extracted using solvent extraction method and biodiesel by acid catalysed transesterification reaction is produced. The species population density is found to be exponential at 7130 LUX at a photoperiod of 8/16(L/D). The biomass growth in the developed design is established as a factor of 2.88 with respect to optical density. At the light intensity of 7130LUX, the developed Hybrid Photobioreactor yields a dry algal mass of 2.09g/L with a lipid productivity of 1.01gL-1day-1 and 70.62% of fatty acid methyl esters (FAME). The analysis of FAME is done using gas chromatography and a maximum of 73.4% biodiesel composition is observed.


2017 ◽  
Vol 26 ◽  
pp. 312-322 ◽  
Author(s):  
Ali Parsaeimehr ◽  
Elena I. Mancera-Andrade ◽  
Felipe Robledo-Padilla ◽  
Hafiz M.N. Iqbal ◽  
Roberto Parra-Saldivar

2018 ◽  
Vol 85 (4) ◽  
Author(s):  
Ming-Hua Liang ◽  
Lu-Lu Xue ◽  
Jian-Guo Jiang

ABSTRACT Microalgae are promising alternatives for sustainable biodiesel production. Previously, it was found that 100 ppm triethylamine greatly enhanced lipid production and lipid content per cell of Dunaliella tertiolecta by 20% and 80%, respectively. However, triethylamine notably reduced biomass production and pigment contents. In this study, a two-stage cultivation with glycerol and triethylamine was attempted to improve cell biomass and lipid accumulation. At the first stage with 1.0 g/liter glycerol addition, D. tertiolecta cells reached the late log phase in a shorter time due to rapid cell growth, leading to the highest cell biomass (1.296 g/liter) for 16 days. However, the increased glycerol concentrations with glycerol addition decreased the lipid content. At the second-stage cultivation with 100 ppm triethylamine, the highest lipid concentration and lipid weight content were 383.60 mg/liter and 37.7% of dry cell weight (DCW), respectively, in the presence of 1.0 g/liter glycerol, which were 27.36% and 72.51% higher than those of the control group, respectively. Besides, the addition of glycerol alleviated the inhibitory effect of triethylamine on cell morphology, algal growth, and pigment accumulation in D. tertiolecta. The results indicated that two-stage cultivation is a viable way to improve lipid yield in microalgae. IMPORTANCE Microalgae are promising alternatives for sustainable biodiesel production. Two-stage cultivation with glycerol and triethylamine enhanced the lipid productivity of Dunaliella tertiolecta, indicating that two-stage cultivation is an efficient strategy for biodiesel production from microalgae. It was found that glycerol significantly enhanced cell biomass of D. tertiolecta, and the presence of glycerol alleviated the inhibitory effect of triethylamine on algal growth. Glycerol, the major byproduct from biodiesel production, was used for the biomass accumulation of D. tertiolecta at the first stage of cultivation. Triethylamine, as a lipid inducer, was used for lipid accumulation at the second stage of cultivation. Two-stage cultivation with glycerol and triethylamine enhanced lipid productivity and alleviated the inhibitory effect of triethylamine on the algal growth of D. tertiolecta, which is an efficient strategy for lipid production from D. tertiolecta.


2021 ◽  
Vol 228 ◽  
pp. 113655 ◽  
Author(s):  
Ashwin Jacob ◽  
B. Ashok ◽  
Avinash Alagumalai ◽  
Ong Hwai Chyuan ◽  
Phung Thi Kim Le

Energies ◽  
2019 ◽  
Vol 12 (14) ◽  
pp. 2660 ◽  
Author(s):  
Muhammad Maqsood Alam ◽  
Abdul Samad Mumtaz ◽  
Megan Russell ◽  
Melanie Grogger ◽  
Don Veverka ◽  
...  

Production of microalgae as feedstock for biofuels must deal with a number of challenges including constraints imposed by local conditions. One solution is to use indigenous strains adapted to local climatic conditions. The present report describes the isolation, identification, and characterization of 32 microalgal strains from different ecological habitats: desert freshwater channels, northern region, and saline regions of Pakistan. The effects of temperature on algal growth rates, biomass productivity, and lipid content were determined through growth at 12, 20, and 35 °C for 15 days under 2% CO2 Responses to temperature varied among species with 20 °C being the optimum temperature in general, although, exceptionally, the best overall growth rate was found for strain S29 (0.311 d−1) at 12 °C. In some cases high biomass productivity was observed at 35 °C, and, depending upon the strain, the maximum lipid content was obtained at different temperatures, including 12 °C. Fatty acid methyl ester (FAME) analysis showed that the major fatty acids present were palmitic, stearic, oleic, linoleic, and linolenic. Oleic acid (C18:1) was the predominant fatty acid, with the specific FAME profile varying with strain. Thus, there is a rich diversity of microalgal strains native to Pakistan, some of which, characterized here, could be suitable for biodiesel production or other biotechnological applications.


2019 ◽  
Vol 7 ◽  
pp. 100227 ◽  
Author(s):  
Guo Yong Yew ◽  
Sze Ying Lee ◽  
Pau Loke Show ◽  
Yang Tao ◽  
Chung Lim Law ◽  
...  

2020 ◽  
Vol 10 (11) ◽  
pp. 3883
Author(s):  
Torben Schädler ◽  
Anna-Cathrine Neumann-Cip ◽  
Karin Wieland ◽  
David Glöckler ◽  
Christoph Haisch ◽  
...  

(1) Background: Recycling of water and non-converted nutrients is considered to be a necessity for an economically viable production of microalgal biomass as a renewable feedstock. However, medium recycling might also have a negative impact on algal growth and productivity due to the accumulation of growth-inhibiting substances. (2) Methods: Consecutive batch processes with repeated water recycling after harvesting of algal biomass were performed with the saline microalga Microchloropsis salina in open thin-layer cascade photobioreactors operated at a physically simulated Mediterranean summer climate. The impact of water recycling on culture performance was studied and the composition of the recycled water was analyzed. (3) Results: Water recycling had no adverse effect on microalgal growth and biomass productivity (14.9−21.3 g m−2 d−1) if all necessary nutrients were regularly replenished and KNO3 was replaced by urea as the nitrogen source to prevent the accumulation of K+ ions. Dissolved organic carbon accumulated in recycled water, probably promoting mixotrophic growth. (4) Conclusion: This study shows that repeated recycling of water is feasible even in high-density cultivation processes with M. salina of more than 30 g L−1 cell dry weight, increasing culture performance while reducing nutrient consumption and circumventing wastewater production.


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