scholarly journals P16.15: First trimester prenatal diagnosis of fetal RhD status and gender by real-time PCR in maternal serum

2005 ◽  
Vol 26 (4) ◽  
pp. 471-471
Author(s):  
G. Pescia ◽  
P. J. Ditesheim ◽  
N. Roussy ◽  
D. Schmid ◽  
R. Coquoz
Keyword(s):  
Prenatal Diagnosis ◽  
Real Time ◽  
Real Time Pcr ◽  
First Trimester ◽  
Maternal Serum ◽  
And Gender

First-trimester fetal sex determination in maternal serum using real-time PCR

2001 ◽  
Vol 21 (12) ◽  
pp. 1070-1074 ◽  
Author(s):  
Jean-Marc Costa ◽  
Alexandra Benachi ◽  
Evelyne Gautier ◽  
Jean-Marie Jouannic ◽  
Pauline Ernault ◽  
...  
Keyword(s):  
Sex Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
First Trimester ◽  
Maternal Serum ◽  
Fetal Sex

First Trimester Maternal Serum Lysosomal Enzymes: Implications for Carrier Testing and Prenatal Diagnosis

1989 ◽  
Vol 4 (4) ◽  
pp. 161-165
Author(s):  
Robert A. Kanfmann ◽  
Arie Drugan ◽  
Mark I. Evans ◽  
Deborah Mitchell ◽  
Yoav Ben-Yoseph ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Lysosomal Enzymes ◽  
First Trimester ◽  
Maternal Serum ◽  
Carrier Testing ◽  
Serum Lysosomal Enzymes

scholarly journals Simultaneous Detection of Trisomies 13, 18, and 21 with Multiplex Ligation-Dependent Probe Amplification–Based Real-Time PCR

2010 ◽  
Vol 56 (9) ◽  
pp. 1451-1459 ◽  
Author(s):  
Qiwei Guo ◽  
Yulin Zhou ◽  
Xiaobo Wang ◽  
Qingge Li
Keyword(s):  
Prenatal Diagnosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Karyotype Analysis ◽  
Simultaneous Detection ◽  
Maternal Blood ◽  
Trisomy 13 ◽  
Clinical Samples ◽  
Dependent Probe ◽  
Single Reaction

BACKGROUND Trisomies 13, 18, and 21 account for the majority of chromosomal aneuploidies detected in prenatal diagnosis. Diagnosis of these trisomies relies mainly on karyotype analysis. Several molecular methods have been developed for trisomy detection, but performance or throughput limitations of these methods currently constrain their use in routine testing. METHODS We developed multiplex ligation-dependent probe amplification–based real-time PCR (MLPA/rtPCR) to simultaneously detect these 3 trisomy conditions with a single reaction. We applied the method to DNA isolated from 144 blinded clinical samples that included 32 cases of trisomy 21, 11 cases of trisomy 18, 1 case of trisomy 13, and 100 unaffected control samples; results were compared with karyotype analysis. RESULTS As judged by the results of the karyotype analysis, MLPA/rtPCR correctly detected all 44 cases of trisomy in the analysis of the blinded clinical samples. The method was able to detect a change in chromosome dosage as low as 1.2-fold. CONCLUSIONS This novel PCR-based technology simultaneously identified 3 types of trisomy in a single reaction and accurately detected trisomy with mosaicism, while reducing assay times and costs compared with conventional methods. The MLPA/rtPCR approach may have applicability in noninvasive prenatal diagnosis with maternal blood samples.

Sensitivity of DCSR3/GAPDH Ratio Using Quantitative Real-Time PCR in the Rapid Prenatal Diagnosis for Down Syndrome

2009 ◽  
Vol 25 (2) ◽  
pp. 220-223 ◽  
Author(s):  
Sanaa M.H. Helmy ◽  
Somaya Ismail ◽  
Randa Bassiouni ◽  
Khaled R. Gaber
Keyword(s):  
Down Syndrome ◽  
Prenatal Diagnosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr

scholarly journals Assessment the Possible Association Between Neuromyelitis Optica and Cytomegalovirus as a Provocative Factor

2019 ◽  
Vol 5 (1) ◽  
pp. 1-6
Author(s):  
Mahboubeh Jazini ◽  
◽  
Rasoul Roghanian ◽  
Omid Mirmosayyeb ◽  
Vahid Shaygannejad ◽  
...  
Keyword(s):  
Real Time ◽  
Neuromyelitis Optica ◽  
Real Time Pcr ◽  
Transverse Myelitis ◽  
Study Groups ◽  
Viral Agent ◽  
Chi Square ◽  
Inflammatory Reactions ◽  
Significant Difference ◽  
And Gender

Background: Neuromyelitis Optica (NMO) is an autoimmune inflammation of the central nervous system in which autoantibodies are released against Aquaporin-4 (AQP-4), astrocytic water channels. The disease is characterized by transverse myelitis and optic neuritis. Viruses could be inflammatory agents in the brain. Due to such inflammatory reactions, autoantibodies would cross the blood brain barrier. Therefore, recognizing the responsible viral agent may help us prevent or treat NMO. Objectives: To investigate the probable association between Cytomegalovirus infection (CMV) and Neuromyelitis Optica. Materials & Methods: This descriptive study was performed on 25 patients with NMO, 30 patients with Multiple Sclerosis (MS) referring to an academic MS Clinic and 30 healthy individuals in Isfahan City, Iran in 2016. In order to detect DNA of CMV in the sera of the studied groups, real-time PCR technique was used with hydrolyzing probes such as TaqMan. Beacon Designer 7 was used to design a primer and probe. The Chi-square test was used for statistical analysis in SPSS 16. Results: Three study groups had no significant difference in terms of age (P=0.33) and gender (P=0.599). All of the samples were negative for CMV DNA. There was no significant difference between three groups of study (P=0.33). Conclusion: Regarding the negative real-time PCR results of all samples, and especially using higher specificity of primers and probes in detecting this virus, it can be inferred that no significant correlation exists between CMV infection and NMO disease.

Novel real-time PCR assay for rapid prenatal diagnosis of Down syndrome: A prospective study of 563 amniocytes

2009 ◽  
Vol 42 (7-8) ◽  
pp. 672-675 ◽  
Author(s):  
Yu-ning Zhu ◽  
Shi-ming Lu ◽  
Jian-fei You ◽  
Bo Zhu ◽  
Mei-yu Yu
Keyword(s):  
Down Syndrome ◽  
Prenatal Diagnosis ◽  
Prospective Study ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
A Prospective Study

scholarly journals Quantitative analysis of the cutaneousMalasseziamicrobiota in 770 healthy Japanese by age and gender using a real-time PCR assay

2010 ◽  
Vol 48 (2) ◽  
pp. 229-233 ◽  
Author(s):  
Takashi Sugita ◽  
Miho Suzuki ◽  
Seiko Goto ◽  
Akemi Nishikawa ◽  
Masataro Hiruma ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Age And Gender ◽  
And Gender

scholarly journals Evaluation of New Commercial Real-Time PCR Quantification Assay for Prenatal Diagnosis of Cytomegalovirus Congenital Infection

2008 ◽  
Vol 46 (6) ◽  
pp. 2078-2080 ◽  
Author(s):  
A. Ducroux ◽  
S. Cherid ◽  
A. Benachi ◽  
Y. Ville ◽  
M. Leruez-Ville
Keyword(s):  
Prenatal Diagnosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Congenital Infection
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