scholarly journals Kinetics of SRY gene appearance in maternal serum: detection by real time PCR in early pregnancy after assisted reproductive technique

2003 ◽  
Vol 18 (8) ◽  
pp. 1733-1736 ◽  
Author(s):  
J. Guibert
Keyword(s):  
Real Time ◽  
Early Pregnancy ◽  
Real Time Pcr ◽  
Maternal Serum ◽  
Sry Gene ◽  
Assisted Reproductive Technique ◽  
Reproductive Technique ◽  
Kinetics Of ◽  
Serum Detection

scholarly journals Replication kinetics of Marek's disease vaccine virus in feathers and lymphoid tissues using PCR and virus isolation

2005 ◽  
Vol 86 (11) ◽  
pp. 2989-2998 ◽  
Author(s):  
Susan J. Baigent ◽  
Lorraine P. Smith ◽  
Richard J. W. Currie ◽  
Venugopal K. Nair
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Virus Isolation ◽  
Marek's Disease ◽  
Pcr Analysis ◽  
Lymphoid Tissues ◽  
Avirulent Strain ◽  
Marek’S Disease ◽  
Virus Load ◽  
Kinetics Of

CVI988 (Rispens), an avirulent strain of Marek's disease virus, is the most widely used vaccine against Marek's disease. The kinetics of replication of CVI988 was examined in tissues of chickens vaccinated at either 1 day or 14 days of age and sampled regularly up to 28 days post-vaccination. Age at vaccination had no significant effect on the kinetics of CVI988 virus replication. During the cytolytic phase of infection (1–7 days), virus levels peaked in the spleen, bursa and thymus with very close correlation among these organs. Virus load in peripheral blood lagged behind and did not reach high levels. Significant numbers of virus genomes were detected in the feather tips only after 7 days, but subsequently rose to levels almost 103-fold greater than in the other tissues. This is the first accurate quantitative data for kinetics of CVI988 replication in a variety of tissues. There was good correlation between data from virus isolation and PCR, with real-time PCR being the preferred method for rapid, accurate and sensitive quantification of virus. Feathers were ideal for non-invasive sampling to detect and measure CVI988 in live chickens and, from 10 days onwards, virus load in feather tips was predictive of virus load in lymphoid tissues where immune responses will occur. The potential for real-time PCR analysis of feather samples for further investigation of the mechanism of vaccinal protection, and to assist optimization of vaccination regimes, is discussed.

scholarly journals Modeling of Phenoxy Acid Herbicide Mineralization and Growth of Microbial Degraders in 15 Soils Monitored by Quantitative Real-Time PCR of the FunctionaltfdAGene

2012 ◽  
Vol 78 (15) ◽  
pp. 5305-5312 ◽  
Author(s):  
Jacob Bælum ◽  
Emmanuel Prestat ◽  
Maude M. David ◽  
Bjarne W. Strobel ◽  
Carsten S. Jacobsen
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Kinetic Models ◽  
Exponential Model ◽  
Dichlorophenoxyacetic Acid ◽  
Order Kinetic ◽  
Clear Trend ◽  
Gene Copies ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Kinetics Of

ABSTRACTMineralization potentials, rates, and kinetics of the three phenoxy acid (PA) herbicides, 2,4-dichlorophenoxyacetic acid (2,4-D), 4-chloro-2-methylphenoxyacetic acid (MCPA), and 2-(4-chloro-2-methylphenoxy)propanoic acid (MCPP), were investigated and compared in 15 soils collected from five continents. The mineralization patterns were fitted by zero/linear or exponential growth forms of the three-half-order models and by logarithmic (log), first-order, or zero-order kinetic models. Prior and subsequent to the mineralization event,tfdAgenes were quantified using real-time PCR to estimate the genetic potential for degrading PA in the soils. In 25 of the 45 mineralization scenarios, ∼60% mineralization was observed within 118 days. Elevated concentrations oftfdAin the range 1 × 105to 5 × 107gene copies g−1of soil were observed in soils where mineralization could be described by using growth-linked kinetic models. A clear trend was observed that the mineralization rates of the three PAs occurred in the order 2,4-D > MCPA > MCPP, and a correlation was observed between rapid mineralization and soils exposed to PA previously. Finally, for 2,4-D mineralization, all seven mineralization patterns which were best fitted by the exponential model yielded a highertfdAgene potential after mineralization had occurred than the three mineralization patterns best fitted by the Lin model.

First-trimester fetal sex determination in maternal serum using real-time PCR

2001 ◽  
Vol 21 (12) ◽  
pp. 1070-1074 ◽  
Author(s):  
Jean-Marc Costa ◽  
Alexandra Benachi ◽  
Evelyne Gautier ◽  
Jean-Marie Jouannic ◽  
Pauline Ernault ◽  
...  
Keyword(s):  
Sex Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
First Trimester ◽  
Maternal Serum ◽  
Fetal Sex

scholarly journals Development of a Taqman real-time PCR assay for rapid detection and quantification ofVibrio tapetisin extrapallial fluids of clams

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e1484 ◽  
Author(s):  
Adeline Bidault ◽  
Gaëlle G. Richard ◽  
Cédric Le Bris ◽  
Christine Paillard
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sea Water ◽  
Control Measures ◽  
Dilution Factor ◽  
Pcr Assay ◽  
Important Species ◽  
Quantitative Identification ◽  
Template Dna ◽  
Kinetics Of

The Gram-negative bacteriumVibrio tapetisis known as the causative agent of Brown Ring Disease (BRD) in the Manila clamVenerupis(=Ruditapes)philippinarum. This bivalve is the second most important species produced in aquaculture and has a high commercial value. In spite of the development of several molecular methods, no survey has been yet achieved to rapidly quantify the bacterium in the clam. In this study, we developed a Taqman real-time PCR assay targeting virB4 gene for accurate and quantitative identification ofV. tapetisstrains pathogenic to clams. Sensitivity and reproducibility of the method were assessed using either filtered sea water or extrapallial fluids of clam injected with the CECT4600TV. tapetisstrain. Quantification curves ofV. tapetisstrain seeded in filtered seawater (FSW) or extrapallial fluids (EF) samples were equivalent showing reliable qPCR efficacies. With this protocol, we were able to specifically detectV. tapetisstrains down to 1.125 101bacteria per mL of EF or FSW, taking into account the dilution factor used for appropriate template DNA preparation. This qPCR assay allowed us to monitorV. tapetisload both experimentally or naturally infected Manila clams. This technique will be particularly useful for monitoring the kinetics of massive infections byV. tapetisand for designing appropriate control measures for aquaculture purposes.

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