Multiplex PCR-based detection of potential fumonisin-producingFusarium in traditional African vegetables

2006 ◽  
Vol 21 (4) ◽  
pp. 360-366 ◽  
Author(s):  
Cornelius Carlos Bezuidenhout ◽  
Maryn Prinsloo ◽  
Anna Margaretha Van der Walt
Keyword(s):  
Multiplex Pcr ◽  
African Vegetables

A multiplex PCR-SSCP test to genotype bovine β-casein alleles A1 , A2 , A3 , B, and C

1999 ◽  
Vol 30 (4) ◽  
pp. 322-323 ◽  
Author(s):  
A. Barroso ◽  
S. Dunner ◽  
J. Ca·ón
Keyword(s):  
Multiplex Pcr

Multiplex PCR zum Nachweis disseminierter Tumorzellen (DTC) im Blut von Patientinnen mit Mammakarzinom

2005 ◽  
Vol 127 (03) ◽  
Author(s):  
J Rom ◽  
A Schneeeweiss ◽  
V Zieglschmid ◽  
C Hollmann ◽  
O Böcher ◽  
...  
Keyword(s):  
Multiplex Pcr

Usefulness of a Multiplex PCR for Detection of Diarrheagenic Escherichia coli in a Diagnostic Microbiology Laboratory Setting

2016 ◽  
Vol 1 (2) ◽  
pp. 38-42 ◽  
Author(s):  
Khairun Nessa ◽  
Dilruba Ahmed ◽  
Johirul Islam ◽  
FM Lutful Kabir ◽  
M Anowar Hossain
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Clinical Laboratory ◽  
Proteinase K ◽  
Microbiology Laboratory ◽  
Pcr Assay ◽  
E Coli ◽  
Diarrheagenic Escherichia Coli ◽  
Multiplex Pcr Assay ◽  
Diagnostic Microbiology

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42

EVALUATION OF THE ISOLATION AND DETECTION METHODS FOR SALMONELLA SPP. FROM EGG SHELL CONTAMINATION USING MULTIPLEX PCR.

2015 ◽  
Vol 14 (1) ◽  
pp. 282-288
Author(s):  
Israa Adnan Ibraheam Al-Baghdady ◽  
Ashwak Bassim Jassim ◽  
Zainab Khudher Ahmed
Keyword(s):  
Multiplex Pcr ◽  
Detection Methods ◽  
Salmonella Spp ◽  
Egg Shell
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