Conditioned media cause increases in select osteogenic and adipogenic differentiation markers in mesenchymal stem cell cultures

2008 ◽  
Vol 2 (2-3) ◽  
pp. 147-154 ◽  
Author(s):  
Scott Maxson ◽  
Karen J. L. Burg
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Cultures ◽  
Adipogenic Differentiation ◽  
Conditioned Media ◽  
Differentiation Markers

Conditioned media derived from mesenchymal stem cell cultures: The next generation for regenerative medicine

2019 ◽  
Vol 13 (4) ◽  
pp. 569-586 ◽  
Author(s):  
Tarini Nawamalie Abeysinghe Gunawardena ◽  
Mohammad Tariqur Rahman ◽  
Basri Johan Jeet Abdullah ◽  
Noor Hayaty Abu Kasim
Keyword(s):  
Stem Cell ◽  
Regenerative Medicine ◽  
Mesenchymal Stem Cell ◽  
Cell Cultures ◽  
Conditioned Media ◽  
Next Generation

scholarly journals Canine Bone Marrow Mesenchymal Stem Cell Conditioned Media Affect Bacterial Growth, Biofilm-Associated Staphylococcus aureus and AHL-Dependent Quorum Sensing

2020 ◽  
Vol 8 (10) ◽  
pp. 1478 ◽  
Author(s):  
Dobroslava Bujňáková ◽  
Anna Čuvalová ◽  
Milan Čížek ◽  
Filip Humenik ◽  
Michel Salzet ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bone Marrow ◽  
Stem Cell ◽  
Quorum Sensing ◽  
Mesenchymal Stem Cell ◽  
Bacterial Growth ◽  
Conditioned Media ◽  
Amyloid Β Peptide

The present study investigated the in vitro antibacterial, antibiofilm and anti-Quorum Sensing (anti-QS) activities of canine bone marrow mesenchymal stem cell-conditioned media (cBM MSC CM) containing all secreted factors <30 K, using a disc diffusion test (DDT), spectrophotometric Crystal Violet Assay (SCVA) and Bioluminescence Assay (BA) with QS-reporter Escherichia coli JM109 pSB1142. The results show a sample-specific bacterial growth inhibition (zones varied between 7–30 mm), statistically significant modulation of biofilm-associated Staphylococcus aureus and Escherichia coli bioluminescence (0.391 ± 0.062 in the positive control to the lowest 0.150 ± 0.096 in the experimental group, cf. 11,714 ± 1362 to 7753 ± 700, given as average values of absorbance A550 ± SD versus average values of relative light units to growth RLU/A550 ± SD). The proteomic analysis performed in our previous experiment revealed the presence of several substances with documented antibacterial, antibiofilm and immunomodulatory properties (namely, apolipoprotein B and D; amyloid-β peptide; cathepsin B; protein S100-A4, galectin 3, CLEC3A, granulin, transferrin). This study highlights that cBM MSC CM may represent an important new approach to managing biofilm-associated and QS signal molecule-dependent bacterial infections. To the best of our knowledge, there is no previous documentation of canine BM MSC CM associated with in vitro antibiofilm and anti-QS activity.

Adipogenic Differentiation of Individual Mesenchymal Stem Cell on Different Geometric Micropatterns

Langmuir ◽  
2011 ◽  
Vol 27 (10) ◽  
pp. 6155-6162 ◽  
Author(s):  
Wei Song ◽  
Hongxu Lu ◽  
Naoki Kawazoe ◽  
Guoping Chen
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Adipogenic Differentiation

Mesenchymal stem cell‐conditioned media: A novel alternative of stem cell therapy for quality wound healing

2020 ◽  
Vol 235 (7-8) ◽  
pp. 5555-5569 ◽  
Author(s):  
Anand Joseph ◽  
Indu Baiju ◽  
Irfan A. Bhat ◽  
Sriti Pandey ◽  
Mukesh Bharti ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Conditioned Media

Rat mesenchymal stem cell cultures as a model to elucidate the cellular and molecular pathogenesis of bone metaplasia induced by Solanum glaucophyllum intoxication

Toxicon ◽  
2019 ◽  
Vol 169 ◽  
pp. 25-33
Author(s):  
Fabrício Gomes Melo ◽  
Natália Melo Ocarino ◽  
Amanda Maria Sena Reis ◽  
Lorenna Alves Gomes ◽  
Kênia Mara Magalhães Cardoso ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Cultures ◽  
Molecular Pathogenesis ◽  
Bone Metaplasia

Biocompatible Silica Nanoparticles−Insulin Conjugates for Mesenchymal Stem Cell Adipogenic Differentiation

2010 ◽  
Vol 21 (9) ◽  
pp. 1673-1684 ◽  
Author(s):  
Dan Liu ◽  
Xiaoxiao He ◽  
Kemin Wang ◽  
Chunmei He ◽  
Hui Shi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Silica Nanoparticles ◽  
Adipogenic Differentiation

Neural Markers Espression in Rat Bone Marrow Mesenchymal Stem Cell Cultures Treated with Neurosteroids

2010 ◽  
Vol 35 (12) ◽  
pp. 2154-2160 ◽  
Author(s):  
Daniela Bronzi ◽  
Vincenzo Bramanti ◽  
Daniele Tomassoni ◽  
Floriana Laureanti ◽  
Sonia Grasso ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Cultures ◽  
Neural Markers ◽  
Rat Bone
Sign in / Sign up

Export Citation Format

Share Document