Bone marrow stromal and Schwann cells from adult rats can interact synergistically to aid in peripheral nerve repair even without intercellular contact in vitro

2011 ◽  
Vol 6 (7) ◽  
pp. 579-588 ◽  
Author(s):  
Li-Na Zhou ◽  
Ji-Wei Zhang ◽  
Jia-Chuan Wang ◽  
Wang-Long Lei ◽  
Xiao-Lin Liu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Nerve Repair ◽  
Intercellular Contact ◽  
Adult Rats ◽  
Peripheral Nerve Repair

scholarly journals Peripheral Nerve Repair with Cultured Schwann Cells: Getting Closer to the Clinics

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Maria Carolina O. Rodrigues ◽  
Antonio Antunes Rodrigues ◽  
Loren E. Glover ◽  
Julio Voltarelli ◽  
Cesario V. Borlongan
Keyword(s):  
Bone Marrow ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Nerve Repair ◽  
Experimental Studies ◽  
Experimental Treatment ◽  
Mesenchymal Cells ◽  
Nerve Conduits ◽  
Cultured Schwann Cells

Peripheral nerve injuries are a frequent and disabling condition, which affects 13 to 23 per 100.000 persons each year. Severe cases, with structural disruption of the nerve, are associated with poor functional recovery. The experimental treatment using nerve grafts to replace damaged or shortened axons is limited by technical difficulties, invasiveness, and mediocre results. Other therapeutic choices include the adjunctive application of cultured Schwann cells and nerve conduits to guide axonal growth. The bone marrow is a rich source of mesenchymal cells, which can be differentiatedin vitrointo Schwann cells and subsequently engrafted into the damaged nerve. Alternatively, undifferentiated bone marrow mesenchymal cells can be associated with nerve conduits and afterward transplanted. Experimental studies provide evidence of functional, histological, and electromyographical improvement following transplantation of bone-marrow-derived cells in animal models of peripheral nerve injury. This paper focuses on this new therapeutic approach highlighting its direct translational and clinical utility in promoting regeneration of not only acute but perhaps also chronic cases of peripheral nerve damage.

Sensory recovery after cell therapy in peripheral nerve repair: effects of naïve and skin precursor-derived Schwann cells

2014 ◽  
Vol 121 (2) ◽  
pp. 423-431 ◽  
Author(s):  
Antos Shakhbazau ◽  
Chandan Mohanty ◽  
Ranjan Kumar ◽  
Rajiv Midha
Keyword(s):  
Cell Therapy ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Therapeutic Potential ◽  
Thermal Sensation ◽  
Thermal Sensitivity ◽  
Nerve Repair ◽  
Peripheral Nerve Repair ◽  
Sensory Recovery ◽  
Microsurgical Techniques

Object Cell therapy is a promising candidate among biological or technological innovations sought to augment microsurgical techniques in peripheral nerve repair. This report describes long-term functional regenerative effects of cell therapy in the rat injury model with a focus on sensory recovery. Methods Schwann cells were derived from isogenic nerve or skin precursor cells and injected into the transected and immediately repaired sciatic nerve distal to the injury site. Sensory recovery was assessed at weeks 4, 7, and 10. Axonal regeneration was assessed at Week 11. Results By Week 10, thermal sensitivity in cell therapy groups returned to a level indistinguishable from the baseline (p > 0.05). Immunohistochemistry at 11 weeks after injury showed improved regeneration of NF+ and IB4+ axons. Conclusions: The results of this study show that cell therapy significantly improves thermal sensation and the number of regenerated sensory neurons at 11 weeks after injury. These findings contribute to the view of skin-derived stem cells as a reliable source of Schwann cells with therapeutic potential for functional recovery in damaged peripheral nerve.

scholarly journals Electrospun Hyaluronan-Gelatin Nanofibrous Matrix for Nerve Tissue Engineering

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Hau-Min Liou ◽  
Lih-Rou Rau ◽  
Chun-Chiang Huang ◽  
Meng-Ru Lu ◽  
Fu-Yin Hsu
Keyword(s):  
Peripheral Nerve ◽  
Schwann Cells ◽  
Cell Attachment ◽  
Nerve Repair ◽  
Critical Role ◽  
Nerve Tissue ◽  
Growth Environment ◽  
Biological Performance ◽  
Electrospun Gelatin

Schwann cells play a critical role in the repair of the peripheral nerve. The goal of this study was to fabricate electrospun gelatin (Gel) and hyaluronan-gelatin (HA-Gel) composite nanofibers to provide a suitable growth environment for Schwann cells. The fiber diameters of Gel, 0.5 HA-Gel, 1 HA-Gel, and 1.5 HA-Gel were 130 ± 30 nm, 294 ± 87 nm, 362 ± 129 nm, and 224 ± 54 nm, respectively. The biological performance of Gel and HA-Gel was evaluated using anin vitroculture of RT4-D6P2T rat Schwann cells. We found that the cell attachment and proliferation rates were not significantly different on these matrices. However, the Schwann cells displayed better organized F-actin on HA-Gel than on Gel. Moreover, the expression levels of several genes, including Nrg1, GFAP, and P0, were significantly higher on HA-Gel than on Gel. In addition, the levels of Nrg1 and P0 protein expression were also higher on the HA-Gel than on Gel. These results indicate that the hyaluronan-gelatin composite nanofibrous matrix could potentially be used in peripheral nerve repair.

Peripheral nerve regeneration by transplantation of bone marrow stromal cell—derived Schwann cells in adult rats

2004 ◽  
Vol 101 (5) ◽  
pp. 806-812 ◽  
Author(s):  
Toshiro Mimura ◽  
Mari Dezawa ◽  
Hiroshi Kanno ◽  
Hajime Sawada ◽  
Isao Yamamoto
Keyword(s):  
Bone Marrow ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Peripheral Nerve Regeneration ◽  
Adult Rats ◽  
Content Type ◽  
Alternative Method ◽  
Fine Print

Object. Bone marrow stromal cells (BMSCs) can be induced to form Schwann cells by sequentially treating the cells with β-mercaptoethanol and retinoic acid, followed by forskolin and neurotrophic factors including heregulin. In this study the authors made artificial grafts filled with BMSC-derived Schwann cells (BMSC-DSCs) and transplanted them into the transected sciatic nerve in adult rats to evaluate the potential of BMSCs as a novel alternative method of peripheral nerve regeneration. Methods. The BMSC-DSCs were suspended in Matrigel and transferred into hollow fibers (12 mm in length), which were transplanted into the transected sciatic nerve in adult Wistar rats. Six months after cell transplantation, electrophysiological evaluation and walking track analysis were performed. Results of these studies showed significant improvement in motor nerve conduction velocity and sciatic nerve functional index in the BMSC-DSC—transplanted group compared with the control group (Matrigel graft only). Immunohistochemical study data demonstrated that transplanted BMSCs labeled with retrovirus green fluorescent protein were positive for P0 and myelin-associated glycoprotein and had reconstructed nodes of Ranvier and remyelinated regenerated nerve axons. The number of regenerated axons in the axial section of the central portion of the graft was significantly greater in the transplanted group. Although BMSCs can differentiate into several types of cells, tumor formation did not occur 6 months after engraftment. Conclusions. Results in this study indicate that BMSC-DSCs have great potential to promote regeneration of peripheral nerves. The artificial graft made with BMSC-DSCs represents an alternative method for the difficult reconstruction of a long distance gap in a peripheral nerve.

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