Advanced maturation by electrical stimulation: Differences in response between C2C12 and primary muscle progenitor cells

2010 ◽  
Vol 5 (7) ◽  
pp. 529-539 ◽  
Author(s):  
Marloes L. P. Langelaan ◽  
Kristel J. M. Boonen ◽  
Kang Yuen Rosaria-Chak ◽  
Daisy W. J. van der Schaft ◽  
Mark J. Post ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Progenitor Cells ◽  
Muscle Progenitor Cells

scholarly journals Smooth Muscle Progenitor Cells: Friend or Foe in Vascular Disease?

2009 ◽  
Vol 4 (2) ◽  
pp. 131-140 ◽  
Author(s):  
Olivia Oostrom ◽  
Joost Fledderus ◽  
Dominique de Kleijn ◽  
Gerard Pasterkamp ◽  
Marianne Verhaar
Keyword(s):  
Smooth Muscle ◽  
Vascular Disease ◽  
Progenitor Cells ◽  
Muscle Progenitor Cells

scholarly journals Syndecan-4-Expressing Muscle Progenitor Cells in the SP Engraft as Satellite Cells during Muscle Regeneration

2009 ◽  
Vol 4 (3) ◽  
pp. 217-225 ◽  
Author(s):  
Kathleen Kelly Tanaka ◽  
John K. Hall ◽  
Andrew A. Troy ◽  
D.D.W. Cornelison ◽  
Susan M. Majka ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Satellite Cells ◽  
Muscle Regeneration ◽  
Muscle Progenitor Cells

scholarly journals Reduction of Connexin 43 Attenuates Angiogenic Effects of Human Smooth Muscle Progenitor Cells via Inactivation of Akt and NF-κB Pathway

2020 ◽  
Author(s):  
Ting-Yi Tien ◽  
Yih-Jer Wu ◽  
Cheng-Huang Su ◽  
Hsueh-Hsiao Wang ◽  
Chin-Ling Hsieh ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase ◽  
Progenitor Cells ◽  
Connexin 43 ◽  
Paracrine Effects ◽  
Angiogenic Potential ◽  
Junction Protein ◽  
Src Activation ◽  
Muscle Progenitor Cells

Objective: Circulating progenitor cells possess vasculogenesis property and participate in repair of vascular injury. Cx (connexin) 43—a transmembrane protein constituting gap junctions—is involved in vascular pathology. However, the role of Cx43 in smooth muscle progenitor cells (SPCs) remained unclear. Approach and Results: Human SPCs cultured from CD34 + peripheral blood mononuclear cells expressed smooth muscle cell markers, such as smooth muscle MHC (myosin heavy chain), nonmuscle MHC, calponin, and CD140B, and Cx43 was the most abundant Cx isoform. To evaluate the role of Cx43 in SPCs, short interference RNA was used to knock down Cx43 expression. Cellular activities of SPCs were reduced by Cx43 downregulation. In addition, Cx43 downregulation attenuated angiogenic potential of SPCs in hind limb ischemia mice. Protein array and ELISA of the supernatant from SPCs showed that IL (interleukin)-6, IL-8, and HGF (hepatocyte growth factor) were reduced by Cx43 downregulation. Simultaneously, Cx43 downregulation reduced the phosphorylation of NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) and Akt (protein kinase B) pathway and reactivation of NF-κB and Akt using betulinic acid, and SC79 could restore the secretion of growth factors and cytokines. Moreover, FAK (focal adhesion kinase)-Src (proto-oncogene tyrosine-protein kinase Src) activation was increased by Cx43 downregulation, and inactivation of Akt–NF-κB could be restored by Src inhibitor (PP2), indicating that Akt–NF-κB inactivated by Cx43 downregulation arose from FAK-Src activation. Finally, the depressed cellular activities and secretion of SPCs after Cx43 downregulation were restored by FAK inhibitor PF-562271 or PP2. Conclusions: SPCs possess angiogenic potential to repair ischemic tissue mainly through paracrine effects. Gap junction protein Cx43 plays an important role in regulating cellular function and paracrine effects of SPCs through FAK-Src axis.

Large and Small Assembly: Combining Functional Macromolecules with Small Peptides to Control the Morphology of Skeletal Muscle Progenitor Cells

2018 ◽  
Vol 19 (3) ◽  
pp. 825-837 ◽  
Author(s):  
Rui Li ◽  
Natasha L. McRae ◽  
Daniel R. McCulloch ◽  
Mitchell Boyd-Moss ◽  
Colin J. Barrow ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Progenitor Cells ◽  
Small Peptides ◽  
Muscle Progenitor Cells

Skeletal Muscle Progenitor Cells

2012 ◽  
pp. 789-789
Author(s):  
Gordon S. Lynch ◽  
David G. Harrison ◽  
Hanjoong Jo ◽  
Charles Searles ◽  
Philippe Connes ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Progenitor Cells ◽  
Muscle Progenitor Cells
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