Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation

2021 ◽  
Author(s):  
Eren Ozudogru ◽  
Melis Isik ◽  
Cemil Can Eylem ◽  
Emirhan Nemutlu ◽  
Yavuz Emre Arslan ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Extracellular Matrix ◽  
Structure Formation ◽  
Vascular Structure ◽  
Neurogenic Differentiation

scholarly journals Promoting Neuronal Outgrowth Using Ridged Scaffolds Coated with Extracellular Matrix Proteins

Biomedicines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 479
Author(s):  
Ahad M. Siddiqui ◽  
Rosa Brunner ◽  
Gregory M. Harris ◽  
Alan Lee Miller ◽  
Brian E. Waletzki ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Extracellular Matrix ◽  
Schwann Cells ◽  
Cell Attachment ◽  
Dorsal Root Ganglion Neurons ◽  
Neurologic Recovery ◽  
Cord Injury ◽  
Biodegradable Hydrogel ◽  
Ganglion Neurons ◽  
Novel Scaffold

Spinal cord injury (SCI) results in cell death, demyelination, and axonal loss. The spinal cord has a limited ability to regenerate, and current clinical therapies for SCI are not effective in helping promote neurologic recovery. We have developed a novel scaffold biomaterial that is fabricated from the biodegradable hydrogel oligo(poly(ethylene glycol)fumarate) (OPF). We have previously shown that positively charged OPF scaffolds (OPF+) in an open spaced, multichannel design can be loaded with Schwann cells to support axonal generation and functional recovery following SCI. We have now developed a hybrid OPF+ biomaterial that increases the surface area available for cell attachment and that contains an aligned microarchitecture and extracellular matrix (ECM) proteins to better support axonal regeneration. OPF+ was fabricated as 0.08 mm thick sheets containing 100 μm high polymer ridges that self-assemble into a spiral shape when hydrated. Laminin, fibronectin, or collagen I coating promoted neuron attachment and axonal outgrowth on the scaffold surface. In addition, the ridges aligned axons in a longitudinal bipolar orientation. Decreasing the space between the ridges increased the number of cells and neurites aligned in the direction of the ridge. Schwann cells seeded on laminin coated OPF+ sheets aligned along the ridges over a 6-day period and could myelinate dorsal root ganglion neurons over 4 weeks. This novel scaffold design, with closer spaced ridges and Schwann cells, is a novel biomaterial construct to promote regeneration after SCI.

scholarly journals Extracellular matrix-regulated neural differentiation of human multipotent marrow progenitor cells enhances functional recovery after spinal cord injury

2014 ◽  
Vol 14 (10) ◽  
pp. 2488-2499 ◽  
Author(s):  
Win-Ping Deng ◽  
Chi-Chiang Yang ◽  
Liang-Yo Yang ◽  
Chun-Wei D. Chen ◽  
Wei-Hong Chen ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Extracellular Matrix ◽  
Progenitor Cells ◽  
Functional Recovery ◽  
Neural Differentiation ◽  
Cord Injury

The role of extracellular matrix in plasticity in the spinal cord

2014 ◽  
pp. 155-165
Author(s):  
Melissa R. Andrews ◽  
Difei Wang ◽  
Chin Lik Tan ◽  
James W. Fawcett
Keyword(s):  
Spinal Cord ◽  
Extracellular Matrix

scholarly journals “Dress” makes the neuron – different forms of the extracellular matrix in the vertebrate central nervous system

2013 ◽  
Vol 154 (27) ◽  
pp. 1067-1073 ◽  
Author(s):  
Georgina Gáti ◽  
Dávid Lendvai
Keyword(s):  
Spinal Cord ◽  
Central Nervous System ◽  
Extracellular Matrix ◽  
Nervous System ◽  
Human Brain ◽  
Brain Plasticity ◽  
Regional Distribution ◽  
Projection Neurons ◽  
Cortical Region ◽  
Human Central Nervous System

Introduction: Extracellular matrix is a key component of most connective tissues. For decades, the presence of this chemically heterogeneous interface has been largely unaddressed or even denied in the central nervous system. It was not until the end of the last century that scientists turned their attention to this enigmatic substance and unravelled its versatile roles in the developing as well as the adult nervous system. Aim: The aim of the authors was to characterize different parts of the human central nervous system: the hippocampus, the lateral geniculate nucleus and the spinal cord. In addition they looked for connections between brain plasticity and extracellular matrix indifferent animal models. Method: The authors used two perfusion fixed human brain and spinal cord samples, 23 further human brain samples for disease-related investigations, 16 adult rat brains and 18 chicken brains of hatchlings, 13 days or three months of age. They visualized the extracellular matrix via lectin- and immunohistochemistry. Results: It was demonstrated that the human central nervous system shows a bewildering phenotypic versatility in its various parts. The human spinal cord harbours perineuronal nets around long-range projection neurons whilst perisynaptic coats are enriched in the dorsal horn. Periaxonal coats protect functional synapses in neurodegeneration. In the rat thalamus, perineuronal matrix is enriched in less plastic territories and develops in accordance with its linked cortical region. In the chicken, perineuronal matrix is well established already at birth and its further development is not functionally dependent. Conclusions: In human, the perineuronal matrix shows a large diversity depending on regional distribution and function. The authors argue that the development and differentiation of extracellular matrix is strongly linked to those of neurons. This observation was based on findings in the domestic chick which exhibits an immediate maturity after hatching as well as on observations in rat thalamic nuclei which reflect the plasticity of their corresponding cortical fields. Orv. Hetil., 2013, 154, 1067–1073.

scholarly journals The Extracellular Matrix Glycoprotein Tenascin-C Is Beneficial for Spinal Cord Regeneration

2010 ◽  
Vol 18 (10) ◽  
pp. 1769-1777 ◽  
Author(s):  
Jian Chen ◽  
Hyun Joon Lee ◽  
Igor Jakovcevski ◽  
Ronak Shah ◽  
Neha Bhagat ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Extracellular Matrix ◽  
Spinal Cord Regeneration ◽  
Tenascin C

Distribution and synthesis of extracellular matrix proteoglycans, hyaluronan, link proteins and tenascin-R in the rat spinal cord

2008 ◽  
Vol 27 (6) ◽  
pp. 1373-1390 ◽  
Author(s):  
Clare M. Galtrey ◽  
Jessica C. F. Kwok ◽  
Daniela Carulli ◽  
Kate E. Rhodes ◽  
James W. Fawcett
Keyword(s):  
Spinal Cord ◽  
Extracellular Matrix ◽  
Rat Spinal Cord
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